##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781131_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265970
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.29798849494304	34.0	34.0	34.0	33.0	34.0
2	33.28728428018198	34.0	34.0	34.0	33.0	34.0
3	33.33114636989134	34.0	34.0	34.0	33.0	34.0
4	33.392942813099225	34.0	34.0	34.0	33.0	34.0
5	33.384791517840355	34.0	34.0	34.0	33.0	34.0
6	36.82220927172237	38.0	37.0	38.0	34.0	38.0
7	36.71637402714592	38.0	37.0	38.0	34.0	38.0
8	36.511474978381024	38.0	37.0	38.0	34.0	38.0
9	36.47322630371846	38.0	37.0	38.0	34.0	38.0
10-11	36.36962251381735	38.0	37.0	38.0	34.0	38.0
12-13	36.312819114937774	38.0	37.0	38.0	34.0	38.0
14-15	36.32576418393052	38.0	37.0	38.0	34.0	38.0
16-17	36.26663909463473	38.0	37.0	38.0	34.0	38.0
18-19	36.262407414370045	38.0	37.0	38.0	34.0	38.0
20-21	36.21367259465353	38.0	37.0	38.0	33.5	38.0
22-23	36.263725232169044	38.0	37.0	38.0	34.0	38.0
24-25	36.26108771665977	38.0	37.0	38.0	34.0	38.0
26-27	36.02848441553559	38.0	37.0	38.0	32.5	38.0
28-29	35.99460653457157	38.0	37.0	38.0	32.0	38.0
30-31	36.035255855923594	38.0	37.0	38.0	32.0	38.0
32-33	35.990728277625294	38.0	37.0	38.0	32.0	38.0
34-35	36.00982629619882	38.0	37.0	38.0	32.0	38.0
36-37	35.966257472647285	38.0	37.0	38.0	32.0	38.0
38-39	35.901731398278	38.0	37.0	38.0	32.0	38.0
40-41	35.93952889423619	38.0	37.0	38.0	32.0	38.0
42-43	35.93262210023687	38.0	37.0	38.0	32.0	38.0
44-45	35.925292326202204	38.0	37.0	38.0	32.0	38.0
46-47	35.94736624431327	38.0	37.0	38.0	32.0	38.0
48-49	35.92909726660902	38.0	37.0	38.0	32.0	38.0
50-51	35.83008797984735	38.0	36.0	38.0	31.0	38.0
52-53	35.86823513930142	38.0	36.0	38.0	32.0	38.0
54-55	35.87453848178366	38.0	37.0	38.0	32.0	38.0
56-57	35.83390419972177	38.0	36.0	38.0	31.0	38.0
58-59	35.83919239011919	38.0	36.0	38.0	31.5	38.0
60-61	35.826290559085606	38.0	36.0	38.0	31.0	38.0
62-63	35.81393766214235	38.0	36.0	38.0	31.0	38.0
64-65	35.76164981012896	38.0	36.0	38.0	31.0	38.0
66-67	35.74107982103244	38.0	36.0	38.0	31.0	38.0
68-69	35.657476407113585	38.0	36.0	38.0	31.0	38.0
70-71	35.555523179305936	38.0	36.0	38.0	31.0	38.0
72-73	35.46985938263714	38.0	36.0	38.0	31.0	38.0
74-75	35.46856976350716	38.0	36.0	38.0	31.0	38.0
76	34.54771590780915	37.0	34.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	4.0
21	11.0
22	23.0
23	71.0
24	163.0
25	334.0
26	727.0
27	1297.0
28	2174.0
29	3458.0
30	5345.0
31	8205.0
32	11507.0
33	16077.0
34	22190.0
35	30839.0
36	46594.0
37	116947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.186787983607175	17.98774297853141	9.308192653306763	26.51727638455465
2	24.00308305447983	20.435387449712373	30.836184532090083	24.725344963717713
3	19.71312554047449	22.380719630033465	29.8481031695304	28.058051659961652
4	13.403391359927811	17.941121179080348	37.85727713651916	30.798210324472684
5	12.610068804752416	34.79978944993796	37.442192728503215	15.147949016806406
6	28.802120539910515	39.943226679700714	17.930593675978493	13.32405910441027
7	28.17535812309659	30.111666729330377	22.11452419445802	19.598450953115012
8	25.088167838478025	35.861939316464266	18.940482009249163	20.10941083580855
9	24.34259502951461	16.06572169793586	20.538030604955445	39.05365266759409
10-11	24.373237583186075	25.39290145505132	28.14339963153739	22.090461330225214
12-13	24.485844268150544	23.485355491220812	27.046847388803247	24.981952851825394
14-15	22.744858442681508	24.043689137872693	23.728240027070722	29.48321239237508
16-17	23.780313569199535	27.62228822799564	24.91615595743881	23.68124224536602
18-19	23.329510847088017	26.88423506410497	26.08922058878821	23.6970335000188
20-21	22.287100048877694	26.875775463398128	25.98939730044742	24.84772718727676
22-23	22.608188893484225	27.286536075497235	26.47629431890815	23.62898071211039
24-25	23.044140316577057	27.563822987554985	26.25352483362785	23.1385118622401
26-27	22.30853103733504	27.752002105500623	26.62461931796819	23.31484753919615
28-29	22.738090762116027	26.449787570026693	27.857277136519155	22.95484453133812
30-31	22.621912245742003	26.39602210775651	27.66345828476896	23.31860736173253
32-33	22.104184682482988	27.76948528029477	27.283152235214498	22.843177802007745
34-35	23.10974921983682	26.175508515998047	28.1255404744896	22.589201789675528
36-37	23.052223935030266	27.320750460578257	26.327217355340828	23.299808249050642
38-39	22.071098244162876	27.24480204534346	27.35101703199609	23.333082678497576
40-41	22.31623867353461	25.806857916306353	28.10279354814453	23.774109862014512
42-43	23.16520660224837	25.96683836522916	27.569838703613193	23.298116328909277
44-45	21.733090198142648	26.979170583148477	27.684513290972667	23.60322592773621
46-47	22.654434710681656	25.617174869346165	27.314922735646878	24.413467684325298
48-49	22.201564086175132	25.66135278414859	27.82231078693086	24.31477234274542
50-51	21.54566304470429	25.476181524232054	28.246230777907282	24.73192465315637
52-53	21.199571380230854	25.312817235026507	29.61349024326052	23.87412114148212
54-55	20.40418092266045	25.931308042260405	29.07790352295372	24.58660751212543
56-57	20.964770462834153	25.81005376546227	28.244162875512274	24.9810128961913
58-59	20.41376847012821	25.752340489528898	28.993495507012067	24.840395533330828
60-61	20.882618340414332	26.134902432605184	28.07891867503854	24.903560551941947
62-63	19.290333496258977	26.85885626198443	28.257134263262774	25.593675978493813
64-65	19.789449937962928	27.349701094108358	27.212279580403802	25.648569387524912
66-67	19.98909651464451	27.190472609692822	26.701883671090727	26.118547204571946
68-69	19.816896642478472	27.453096213858707	26.446591720870778	26.283415422792046
70-71	20.25999172839042	26.858668270857617	26.406549610858367	26.4747903898936
72-73	19.97762905590856	25.91175696507125	26.472346505244953	27.638267473775237
74-75	19.740384253863215	25.967966311990075	27.01169304808813	27.27995638605858
76	20.129713877504983	25.850283866601497	26.96770312441253	27.052299131480993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	1.5
14	5.0
15	7.5
16	20.5
17	36.0
18	38.0
19	67.5
20	96.0
21	97.0
22	140.5
23	265.5
24	462.0
25	577.0
26	731.5
27	970.0
28	1362.0
29	1670.0
30	1890.5
31	2353.5
32	3436.0
33	4276.0
34	4688.0
35	5844.0
36	7582.0
37	8576.0
38	9849.0
39	12673.5
40	15302.5
41	16906.5
42	17433.0
43	17890.0
44	18335.0
45	18270.5
46	18218.0
47	18122.0
48	16814.0
49	15061.0
50	14520.0
51	13235.5
52	10627.5
53	8615.0
54	7926.0
55	7309.0
56	6013.0
57	4608.0
58	3882.0
59	3293.0
60	2499.5
61	2093.5
62	1892.0
63	1760.0
64	1563.0
65	1453.0
66	1417.5
67	1427.0
68	1418.5
69	1404.5
70	1344.5
71	1290.0
72	1258.0
73	1256.0
74	1194.0
75	1102.0
76	998.5
77	840.0
78	740.0
79	695.0
80	612.0
81	465.0
82	318.5
83	236.0
84	188.0
85	112.5
86	62.0
87	39.0
88	33.5
89	22.5
90	12.5
91	6.0
92	4.0
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	265970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.32646539083355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.017751053181144	18.488175358123097
2	17.73425029105006	11.111027559499192
3	7.4544821709334	7.0056773320299275
4	3.57421476493957	4.478700605331428
5	2.0055449537320422	3.141331729142385
6	1.3790371943974362	2.5920216565778094
7	1.0753849662141888	2.3581606948152047
8	0.8233416147577383	2.063390607963304
9	0.711722416255596	2.0066172876640223
>10	5.793396464191841	35.722825882618345
>50	0.3816656464911965	7.923074030905742
>100	0.048008257420276285	2.004737376395834
>500	0.0	0.0
>1k	0.0012002064355069073	1.1042598789337144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT	2937	1.1042598789337144	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG	350	0.1315937887731699	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC	287	0.10790690679399932	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015039290145505131	0.0
2	0.0	0.0	0.0	0.0015039290145505131	0.0
3	0.0	0.0	0.0	0.0018799112681881416	0.0
4	0.0	0.0	0.0	0.00225589352182577	0.0
5	0.0	0.0	0.0	0.002631875775463398	0.0
6	0.0	0.0	0.0	0.002631875775463398	0.0
7	0.0	0.0	0.0	0.002631875775463398	0.0
8	0.0	0.0	0.0	0.002631875775463398	0.0
9	0.0	0.0	0.0	0.003383840282738655	0.0
10	0.0	0.0	0.0	0.003383840282738655	0.0
11	0.0	0.0	0.0	0.003759822536376283	0.0
12	0.0	0.0	0.0	0.003759822536376283	0.0
13	0.0	0.0	0.0	0.003759822536376283	0.0
14	0.0	0.0	0.0	0.003759822536376283	0.0
15	0.0	0.0	0.0	0.003759822536376283	0.0
16	0.0	0.0	0.0	0.004135804790013911	0.0
17	0.0	0.0	0.0	0.00451178704365154	0.0
18	0.0	0.0	0.0	0.00451178704365154	0.0
19	0.0	0.0	0.0	0.004887769297289168	0.0
20	0.0	0.0	0.0	0.004887769297289168	0.0
21	0.0	0.0	0.0	0.004887769297289168	0.0
22	0.0	0.0	0.0	0.005639733804564425	0.0
23	0.0	0.0	0.0	0.00676768056547731	0.0
24	0.0	0.0	0.0	0.00676768056547731	0.0
25	0.0	0.0	0.0	0.00676768056547731	0.0
26	0.0	0.0	0.0	0.00864759183366545	0.0
27	0.0	0.0	0.0	0.013159378877316991	0.0
28	0.0	0.0	0.0	0.02218295296462007	0.0
29	0.0	0.0	0.0	0.03759822536376283	0.0
30	0.0	0.0	0.0	0.04774974621197879	0.0
31	0.0	0.0	0.0	0.06542091213294733	0.0
32	0.0	0.0	0.0	0.08346806030755348	0.0
33	0.0	0.0	0.0	0.10452306651126067	0.0
34	0.0	0.0	0.0	0.15377674173778996	0.0
35	0.0	0.0	0.0	0.22746926345076512	0.0
36	0.0	0.0	0.0	0.3440237620784299	0.0
37	0.0	0.0	0.0	0.5327668534045193	0.0
38	0.0	0.0	0.0	0.7760273715080648	0.0
39	0.0	0.0	0.0	1.0662856713163138	0.0
40	0.0	0.0	0.0	1.3648155807045907	0.0
41	0.0	0.0	0.0	1.683272549535662	0.0
42	0.0	0.0	0.0	2.0611347144414784	0.0
43	0.0	0.0	0.0	2.510433507538444	0.0
44	0.0	0.0	0.0	3.1056134150468098	0.0
45	0.0	0.0	0.0	3.8372748806256345	0.0
46	0.0	0.0	0.0	4.655036282287476	0.0
47	0.0	0.0	0.0	5.486333045080272	0.0
48	0.0	0.0	0.0	6.239425499116441	0.0
49	0.0	0.0	0.0	6.950407940745197	0.0
50	0.0	0.0	0.0	7.630183855322029	0.0
51	0.0	0.0	0.0	8.284016994397865	0.0
52	0.0	0.0	0.0	8.913035304733617	0.0
53	0.0	0.0	0.0	9.520998608865662	0.0
54	0.0	0.0	0.0	10.2218295296462	0.0
55	0.0	0.0	0.0	10.987329398052411	0.0
56	0.0	0.0	0.0	11.775388201676881	0.0
57	0.0	0.0	0.0	12.616836485317894	0.0
58	0.0	0.0	0.0	13.4082791292251	0.0
59	0.0	0.0	0.0	14.164379441290372	0.0
60	0.0	0.0	0.0	14.902056622927399	0.0
61	0.0	0.0	0.0	15.594615934127908	0.0
62	0.0	0.0	0.0	16.25032898447193	0.0
63	0.0	0.0	0.0	16.887242922134075	0.0
64	0.0	0.0	0.0	17.56777080121818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	25	2.3876855E-4	55.999996	36
AATATCG	25	2.3876855E-4	55.999996	36
ATATCGT	25	2.3876855E-4	55.999996	37
AGCCACG	20	0.0069461926	52.5	44
CTCTCGG	20	0.0069461926	52.5	32
TCGTATG	425	0.0	51.058823	40
CTCGTAT	395	0.0	50.506332	39
TATGCCG	430	0.0	50.46512	43
ATCGTAT	35	2.0831096E-5	50.0	39
CGTATGC	435	0.0	49.88506	41
CCGTCTT	435	0.0	49.080463	47
CGTCTTC	435	0.0	48.275864	48
ATCTCGT	370	0.0	48.243244	37
GCCGTCT	445	0.0	47.977528	46
AATCTCG	380	0.0	47.894733	36
TCTCGTA	375	0.0	47.599995	38
TCTTCTG	415	0.0	47.228916	50
ATGCCGT	470	0.0	46.914894	44
TAGGTTC	30	5.87121E-4	46.666668	45
AACTCGT	30	5.87121E-4	46.666668	37
>>END_MODULE