##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781130_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35764
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.8916508220557	32.0	21.0	34.0	12.0	34.0
2	27.861984118107593	32.0	23.0	34.0	12.0	34.0
3	28.38521977407449	33.0	23.0	34.0	11.0	34.0
4	28.57817917458897	33.0	23.0	34.0	11.0	34.0
5	28.244603511911418	33.0	23.0	34.0	11.0	34.0
6	30.422519852365507	34.0	26.0	37.0	11.0	38.0
7	29.893104798121016	34.0	24.0	37.0	12.0	38.0
8	29.931215747679232	34.0	25.0	37.0	11.0	38.0
9	30.673526451179956	35.0	26.0	37.0	12.0	38.0
10-11	30.493582932557878	34.5	26.5	37.0	11.0	38.0
12-13	30.53966278939716	35.0	26.0	37.0	11.0	38.0
14-15	30.597807851470755	35.0	26.0	37.0	11.0	38.0
16-17	30.51693043283749	35.0	26.0	37.0	11.0	38.0
18-19	30.587811766021698	35.0	26.0	37.0	11.0	38.0
20-21	30.52266245386422	35.0	26.0	37.0	11.0	38.0
22-23	30.63552734593446	35.0	26.5	37.0	11.0	38.0
24-25	30.592649032546696	35.0	26.0	37.0	11.0	38.0
26-27	30.476316966782242	35.0	26.0	37.0	11.0	38.0
28-29	30.586986914215412	35.0	26.0	37.0	11.0	38.0
30-31	30.679482160832123	35.0	26.5	37.0	11.0	38.0
32-33	30.611201207918576	35.0	26.0	37.0	11.0	38.0
34-35	30.569133765798007	35.0	26.0	37.0	11.0	38.0
36-37	30.449012973940278	35.0	26.0	37.0	11.0	38.0
38-39	30.407938150095067	34.5	26.0	37.0	11.0	38.0
40-41	30.342816799015772	34.0	26.0	37.0	11.0	38.0
42-43	30.50574600156582	34.5	26.0	37.0	11.0	38.0
44-45	30.408469410580473	34.0	26.0	37.0	11.0	38.0
46-47	30.334470417179286	34.0	25.5	37.0	11.0	38.0
48-49	30.16129347947657	34.0	25.0	37.0	11.0	38.0
50-51	30.007311821943855	34.0	24.5	37.0	11.0	38.0
52-53	30.10057599821049	34.0	25.0	37.0	11.0	38.0
54-55	29.99117827983447	34.0	25.0	37.0	11.0	38.0
56-57	29.903268650039145	34.0	24.5	37.0	11.0	38.0
58-59	29.89729895984789	34.0	24.0	37.0	11.0	38.0
60-61	29.883653953696452	34.0	24.5	37.0	11.0	38.0
62-63	29.874818252991837	34.0	24.0	37.0	11.0	38.0
64-65	29.87230175595571	34.0	24.5	37.0	11.0	38.0
66-67	29.70460518957611	34.0	24.0	37.0	11.0	38.0
68-69	29.335253327368303	34.0	23.5	37.0	11.0	38.0
70-71	29.496812437087577	34.0	24.0	37.0	11.0	38.0
72-73	29.470682809529137	34.0	24.0	37.0	11.0	38.0
74-75	29.46886533944749	34.0	24.0	37.0	11.0	38.0
76	27.87188233978302	32.0	21.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	13.0
15	35.0
16	128.0
17	245.0
18	491.0
19	668.0
20	817.0
21	969.0
22	1108.0
23	1282.0
24	1374.0
25	1434.0
26	1660.0
27	1772.0
28	1853.0
29	2008.0
30	2144.0
31	2255.0
32	2350.0
33	2774.0
34	2793.0
35	3017.0
36	2884.0
37	1683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60507773179734	19.581143048875965	11.223576781120679	28.59020243820602
2	22.51146404205346	20.892517615479253	30.676098870372442	25.919919472094843
3	18.188681355553072	21.359467621071467	29.7729560451851	30.678894978190357
4	12.88446482496365	17.34984901017783	35.44346269992171	34.322223464936805
5	12.4762330835477	32.893412369980986	36.304663907840286	18.325690638631027
6	27.821272788278716	37.579689072810645	18.708757409685717	15.890280729224918
7	27.605972486299073	28.221116206240914	22.069679006822504	22.10323230063751
8	24.203109271893524	34.713678559445256	19.57275472542221	21.510457443239012
9	23.38384968124371	17.0562576892965	20.80863438094173	38.75125824851806
10-11	23.154568840174477	25.170562576892962	27.456380718040485	24.218487864892072
12-13	23.66765462476233	23.034336204004028	27.200536852701042	26.097472318532606
14-15	21.96342690974164	24.491108377139025	24.358293255787945	29.187171457331395
16-17	22.405211944972596	27.24667263169668	25.304775752153002	25.043339671177723
18-19	21.023934682921375	27.028576221899115	26.98104238899452	24.966446706184993
20-21	21.352477351526673	27.172575774521867	26.539257353763563	24.9356895201879
22-23	21.825019572754726	26.26663684151661	27.22989598478917	24.678447600939492
24-25	21.265798009171235	27.33195392014316	26.898557208365954	24.50369086231965
26-27	20.72335309249525	27.531875629124258	27.35572083659546	24.389050441785034
28-29	21.388826753159602	27.078906162621628	28.351135219774076	23.181131864444694
30-31	21.27838049435186	27.169779666703946	27.32775975841629	24.224080080527905
32-33	20.842187674756737	27.40045856168214	27.369701375685047	24.387652387876077
34-35	21.805446818029303	27.05374119226037	27.2257018230623	23.915110166648027
36-37	21.82222346493681	27.043954814897663	27.20193490661	23.93188681355553
38-39	21.44614696342691	27.70942847556202	27.712224583379935	23.13219997763114
40-41	21.44335085560899	27.168381612794988	27.49832233530925	23.889945196286767
42-43	21.107817917458895	27.091488647802258	27.847835812548933	23.95285762218991
44-45	20.769488871490886	27.185158259702497	27.565428922939265	24.479923945867352
46-47	21.257409685717484	26.307180404876412	27.558438653394475	24.876971256011633
48-49	20.72335309249525	26.588189240577115	28.306397494687396	24.38206017224024
50-51	21.36785594452522	26.10026842635052	27.863214405547478	24.668661223576784
52-53	21.239234984901017	26.03455989262946	27.89397159154457	24.832233530924952
54-55	20.48708198188122	26.634325019572753	28.021194497259817	24.85739850128621
56-57	20.351470752712224	26.52807292249189	27.810088357007047	25.31036796778884
58-59	20.657644558774184	26.705625768929647	27.856224136002684	24.78050553629348
60-61	20.264791410356782	26.388267531596014	28.527290012302874	24.819651045744322
62-63	19.49725981433844	26.26663684151661	28.4350184543116	25.801084889833355
64-65	20.426965663795997	26.68185885247735	28.215523990605078	24.675651493121574
66-67	19.96001565820378	26.55183983894419	28.144223241248184	25.343921261603846
68-69	19.4748909517951	26.488927413041047	27.963874286992507	26.072307348171346
70-71	20.559780785147076	26.262442679789732	28.579018006934348	24.598758528128844
72-73	20.45632479588413	25.731182194385415	27.756962308466615	26.05553070126384
74-75	20.331897997986804	25.926909741639637	27.786321440554744	25.954870819818815
76	20.02852029974276	25.740968571748123	28.04496141371211	26.185549714797002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.5
6	1.5
7	1.0
8	1.0
9	1.0
10	0.5
11	1.0
12	2.0
13	2.0
14	2.5
15	6.0
16	10.0
17	11.0
18	16.0
19	24.0
20	25.5
21	24.0
22	40.0
23	72.5
24	106.0
25	123.0
26	159.0
27	209.5
28	263.0
29	302.0
30	348.5
31	457.5
32	641.5
33	763.0
34	814.0
35	960.5
36	1138.0
37	1220.0
38	1383.0
39	1675.5
40	1941.0
41	2168.0
42	2259.0
43	2303.0
44	2308.0
45	2242.5
46	2216.0
47	2160.0
48	2004.5
49	1791.5
50	1678.0
51	1592.5
52	1368.0
53	1128.5
54	1028.0
55	934.5
56	763.5
57	604.0
58	522.0
59	457.0
60	408.5
61	369.0
62	313.0
63	285.5
64	258.0
65	252.0
66	241.0
67	236.0
68	224.5
69	213.5
70	204.0
71	194.0
72	192.0
73	182.0
74	161.5
75	149.0
76	135.0
77	110.5
78	91.0
79	82.0
80	77.0
81	65.5
82	51.5
83	44.0
84	35.5
85	22.0
86	15.0
87	13.0
88	11.5
89	9.0
90	8.0
91	4.0
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	35764.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.66972374454758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.06852465670026	73.63270327703836
2	6.561591016708382	10.848898333519742
3	2.188324426706352	5.427245274577787
4	1.014679023202327	3.355329381500951
5	0.4938104579584658	2.041158707079745
6	0.22661164851518634	1.1240353428028185
7	0.14881959006967463	0.8612012079185773
8	0.09808563890955828	0.6486970137568504
9	0.04058716092809308	0.30197964433508556
>10	0.15896638030169788	1.7587518174700816
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.002796107817917459	0.0
29	0.0	0.0	0.0	0.005592215635834918	0.0
30	0.0	0.0	0.0	0.008388323453752377	0.0
31	0.0	0.0	0.0	0.008388323453752377	0.0
32	0.0	0.0	0.0	0.008388323453752377	0.0
33	0.0	0.0	0.0	0.011184431271669836	0.0
34	0.0	0.0	0.0	0.013980539089587294	0.0
35	0.0	0.0	0.0	0.02516497036125713	0.0
36	0.0	0.0	0.0	0.039145509450844426	0.0
37	0.0	0.0	0.0	0.05592215635834918	0.0
38	0.0	0.0	0.0	0.08667934235544122	0.0
39	0.0	0.0	0.0	0.15378592998546023	0.0
40	0.0	0.0	0.0	0.2097080863438094	0.0
41	0.0	0.0	0.0	0.304775752153003	0.0
42	0.0	0.0	0.0	0.4026395257801141	0.0
43	0.0	0.0	0.0	0.5480371323118219	0.0
44	0.0	0.0	0.0	0.713007493568952	0.0
45	0.0	0.0	0.0	0.8556089922827425	0.0
46	0.0	0.0	0.0	1.054132647354882	0.0
47	0.0	0.0	0.0	1.261044625880774	0.0
48	0.0	0.0	0.0	1.5182865451291803	0.0
49	0.0	0.0	0.0	1.7699362487417514	0.0
50	0.0	0.0	0.0	1.974052119449726	0.0
51	0.0	0.0	0.0	2.2508667934235542	0.0
52	0.0	0.0	0.0	2.4633709875852814	0.0
53	0.0	0.0	0.0	2.6898557208365954	0.0
54	0.0	0.0	0.0	2.9415054244491667	0.0
55	0.0	0.0	0.0	3.2742422547813446	0.0
56	0.0	0.0	0.0	3.5454647131193378	0.0
57	0.0	0.0	0.0	3.844648249636506	0.0
58	0.0	0.0	0.0	4.121462923610334	0.0
59	0.0	0.0	0.0	4.429034783581255	0.0
60	0.0	0.0	0.0	4.7729560451851025	0.0
61	0.0	0.0	0.0	5.044178503523096	0.0
62	0.0	0.0	0.0	5.3657309025836035	0.0
63	0.0	0.0	0.0	5.634157253103679	0.0
64	0.0	0.0	0.0	5.924952466167095	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTT	20	0.0068973424	52.5	7
ATGAGTA	20	0.0068973424	52.5	2
TTCATTG	20	0.0068973424	52.5	31
ATCTGTC	60	4.8894866E-4	29.166666	66
TGAGTAG	50	0.007118762	28.000002	3
CCATATA	65	7.808692E-4	26.923079	1
>>END_MODULE