FastQCFastQC Report
Wed 25 May 2016
SRR1781130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34318
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT600.17483536336616354No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT370.10781514074246751No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCT150.002206497670.056
TTTCGTT150.002206497670.018
CGCGGCC200.006894962452.563
CCCTAGA200.006894962452.52
ATAGACC200.006894962452.54
GGGGGAA200.006894962452.564
GCCAACC200.006894962452.512
GACAAAA350.001238816340.00000436
Produced by FastQC (version 0.11.5)