##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781130_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34318
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69462089865377	34.0	34.0	34.0	31.0	34.0
2	32.81315927501603	34.0	34.0	34.0	31.0	34.0
3	32.91704062008276	34.0	34.0	34.0	31.0	34.0
4	33.00609009849059	34.0	34.0	34.0	31.0	34.0
5	32.90075179206247	34.0	34.0	34.0	31.0	34.0
6	35.95192027507431	38.0	36.0	38.0	32.0	38.0
7	35.915525380266914	38.0	36.0	38.0	32.0	38.0
8	35.493968179963865	37.0	36.0	38.0	31.0	38.0
9	35.511713969345536	37.0	36.0	38.0	31.0	38.0
10-11	35.40251471530975	37.0	35.5	38.0	31.0	38.0
12-13	35.32172620782097	37.0	35.0	38.0	31.0	38.0
14-15	35.385628533131296	37.0	35.0	38.0	31.0	38.0
16-17	35.30593274666356	37.0	35.0	38.0	30.5	38.0
18-19	35.318375196689786	37.0	35.0	38.0	31.0	38.0
20-21	35.23324494434408	37.0	35.0	38.0	29.0	38.0
22-23	35.27902267031878	37.0	35.0	38.0	30.5	38.0
24-25	35.274578938166556	37.0	35.0	38.0	30.5	38.0
26-27	34.9911562445364	37.0	35.0	38.0	27.0	38.0
28-29	34.90548691648698	37.0	34.5	38.0	27.0	38.0
30-31	34.93944868582085	37.0	35.0	38.0	27.0	38.0
32-33	34.84710647473629	37.0	34.5	38.0	27.0	38.0
34-35	34.83935544029372	37.0	34.0	38.0	27.0	38.0
36-37	34.81956990500612	37.0	34.0	38.0	27.0	38.0
38-39	34.87923247275482	37.0	34.5	38.0	27.0	38.0
40-41	34.84340579287837	37.0	34.0	38.0	27.0	38.0
42-43	34.7717815723527	37.0	34.0	38.0	27.0	38.0
44-45	34.7779882277522	37.0	34.0	38.0	27.0	38.0
46-47	34.767760358995275	37.0	34.0	38.0	27.0	38.0
48-49	34.71956407716067	37.0	34.0	38.0	27.0	38.0
50-51	34.623448336150126	37.0	34.0	38.0	27.0	38.0
52-53	34.64600209802436	37.0	34.0	38.0	27.0	38.0
54-55	34.65143656390232	37.0	34.0	38.0	27.0	38.0
56-57	34.58195407657789	37.0	34.0	38.0	27.0	38.0
58-59	34.57570371233755	37.0	34.0	38.0	27.0	38.0
60-61	34.61492511218603	37.0	34.0	38.0	27.0	38.0
62-63	34.53989160207472	37.0	34.0	38.0	27.0	38.0
64-65	34.48984497931115	37.0	34.0	38.0	27.0	38.0
66-67	34.50407949181188	37.0	34.0	38.0	27.0	38.0
68-69	34.38306428113526	37.0	34.0	38.0	25.5	38.0
70-71	34.42626318550032	37.0	34.0	38.0	25.5	38.0
72-73	34.41389358354216	37.0	34.0	38.0	25.0	38.0
74-75	34.41677836703771	37.0	34.0	38.0	25.0	38.0
76	33.27067428171805	37.0	33.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	6.0
22	11.0
23	26.0
24	55.0
25	132.0
26	192.0
27	364.0
28	616.0
29	904.0
30	1365.0
31	1829.0
32	2376.0
33	3114.0
34	3937.0
35	5000.0
36	6709.0
37	7679.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62381257649047	20.36540590943528	10.755288769741826	24.25549274433242
2	24.6954950754706	22.402237892651087	29.58214348155487	23.320123550323444
3	20.46739320473221	24.730462148143832	27.70557724809138	27.09656739903258
4	15.33888921265808	17.806981758843758	36.453173261845095	30.40095576665307
5	14.292790955183868	36.27542397575616	34.29104260155021	15.140742467509764
6	31.74136021912699	37.39728422402238	17.565126172853894	13.296229383996735
7	30.622413893583545	29.96095343551489	21.644617984731045	17.77201468617052
8	26.336033568389766	34.86217145521301	19.02208753423859	19.779707442158635
9	25.319074538143248	17.35240981409173	21.160906812751325	36.167608835013695
10-11	25.445830176583716	25.741593332944813	27.044116790022727	21.768459700448744
12-13	25.693513608019114	23.690191736115157	26.962526953785186	23.653767702080543
14-15	23.39297161839268	25.158808788390928	24.474036948540125	26.974182644676265
16-17	23.749927151931928	28.31604405851157	25.374439069875866	22.559589719680634
18-19	23.46873360918468	27.484119121160905	26.625968879305322	22.421178390349088
20-21	23.17297045282359	27.622530450492455	26.550206888513316	22.65429220817064
22-23	23.347805816189755	27.022262369601957	27.277230607844277	22.352701206364006
24-25	23.25893117314529	27.654583600442916	26.880937117547642	22.205548108864154
26-27	22.622239058220174	27.825048079724922	27.080540824057348	22.472172037997552
28-29	23.317209627600676	27.223323037473047	28.01299609534355	21.446471239582728
30-31	23.250189404976982	26.95669910833965	27.415641937175828	22.37746954950755
32-33	22.7723060784428	27.68080890494784	27.18689900343843	22.35998601317093
34-35	23.663966431610234	26.863453581211026	27.344250830467974	22.128329156710763
36-37	23.29681216854129	27.236435689725507	27.25246226470074	22.21428987703246
38-39	22.845154146512034	28.07710239524448	27.494317850690596	21.58342560755289
40-41	22.936942712279272	26.929016842473334	27.937234104551546	22.196806340695847
42-43	22.929657905472347	27.59776210734891	27.223323037473047	22.249256949705693
44-45	22.721312430794335	27.91975056821493	26.90279153796841	22.45614546302232
46-47	23.01124774170989	26.408881636459004	28.030479631680166	22.54939099015094
48-49	22.795617460224953	26.588087883909317	28.416574392447114	22.199720263418616
50-51	22.613497290051868	26.98875225829011	27.281601491928438	23.11614895972959
52-53	22.76647823299726	25.91497173494959	28.87843114400606	22.44011888804709
54-55	22.08170639314645	26.735240981409174	28.470481962818344	22.712570662626028
56-57	22.268197447403697	26.5895448452707	28.072731511160327	23.069526196165278
58-59	22.28568098374031	26.236960195815605	28.47776676962527	22.999592050818812
60-61	21.670843289235968	26.838685238067487	28.295646599452184	23.194824873244364
62-63	21.27309283757795	26.201993123142376	28.8463779940556	23.67853604522408
64-65	21.6679293665132	26.937758610641644	28.072731511160327	23.321580511684832
66-67	21.31097383297395	26.837228276706103	28.08730112477417	23.76449676554578
68-69	21.130310624162245	26.745439710938868	27.94451891135847	24.179730753540415
70-71	21.74951920275074	26.75563844046856	27.90081007051693	23.59403228626377
72-73	21.31971560114226	26.218019698117605	27.539483652893527	24.922781047846613
74-75	21.297861180721487	26.156827320939446	27.71577597762107	24.82953552071799
76	22.279853138294772	26.11457544145929	27.064514249082116	24.54105717116382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	2.0
15	4.0
16	7.5
17	9.0
18	14.0
19	21.0
20	28.5
21	34.0
22	52.5
23	76.0
24	105.0
25	129.0
26	154.0
27	187.5
28	255.5
29	315.0
30	353.0
31	438.0
32	585.5
33	686.0
34	786.5
35	954.5
36	1137.5
37	1253.0
38	1335.5
39	1657.5
40	1992.5
41	2134.0
42	2180.0
43	2215.0
44	2297.5
45	2254.0
46	2163.0
47	2144.0
48	1962.5
49	1719.5
50	1639.0
51	1500.0
52	1217.0
53	994.5
54	916.0
55	822.5
56	660.0
57	528.5
58	466.0
59	398.5
60	325.0
61	307.0
62	295.0
63	276.5
64	232.0
65	215.0
66	224.5
67	225.0
68	229.0
69	222.5
70	202.0
71	192.0
72	186.5
73	188.0
74	171.0
75	147.0
76	126.0
77	99.5
78	92.0
79	90.0
80	76.5
81	51.5
82	33.5
83	27.0
84	21.5
85	14.5
86	9.0
87	5.0
88	6.0
89	6.5
90	3.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	34318.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.56908910775687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36219002755311	49.41430153272336
2	13.972091369656031	18.32274608077394
3	4.821793618345036	9.48481846261437
4	2.2886854501822063	6.002680808904948
5	1.3198826770953693	4.327175243312547
6	0.7199360056883832	2.8323328865318493
7	0.43107279352946404	1.9785535287604172
8	0.3421918051728735	1.7949763972259454
9	0.23109056972713538	1.3637158342560756
>10	0.506621633632566	4.303863861530393
>50	0.0044440494178295264	0.17483536336616354
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT	60	0.17483536336616354	No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT	37	0.10781514074246751	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0029139227227693923	0.0
28	0.0	0.0	0.0	0.0029139227227693923	0.0
29	0.0	0.0	0.0	0.005827845445538785	0.0
30	0.0	0.0	0.0	0.005827845445538785	0.0
31	0.0	0.0	0.0	0.005827845445538785	0.0
32	0.0	0.0	0.0	0.005827845445538785	0.0
33	0.0	0.0	0.0	0.008741768168308177	0.0
34	0.0	0.0	0.0	0.017483536336616354	0.0
35	0.0	0.0	0.0	0.043708840841540884	0.0
36	0.0	0.0	0.0	0.06119237717815724	0.0
37	0.0	0.0	0.0	0.08450375896031237	0.0
38	0.0	0.0	0.0	0.15443790430677778	0.0
39	0.0	0.0	0.0	0.2535112768809371	0.0
40	0.0	0.0	0.0	0.35258464945509643	0.0
41	0.0	0.0	0.0	0.512850399207413	0.0
42	0.0	0.0	0.0	0.690599685296346	0.0
43	0.0	0.0	0.0	0.9033160440585115	0.0
44	0.0	0.0	0.0	1.136429861880063	0.0
45	0.0	0.0	0.0	1.419080365988694	0.0
46	0.0	0.0	0.0	1.6959030246517863	0.0
47	0.0	0.0	0.0	2.0543155195524214	0.0
48	0.0	0.0	0.0	2.4127280144530565	0.0
49	0.0	0.0	0.0	2.7740544320764613	0.0
50	0.0	0.0	0.0	3.0974998543038637	0.0
51	0.0	0.0	0.0	3.4879654991549622	0.0
52	0.0	0.0	0.0	3.8522058395011363	0.0
53	0.0	0.0	0.0	4.163995570837462	0.0
54	0.0	0.0	0.0	4.539891602074713	0.0
55	0.0	0.0	0.0	5.009033160440585	0.0
56	0.0	0.0	0.0	5.414068418905531	0.0
57	0.0	0.0	0.0	5.868640363657556	0.0
58	0.0	0.0	0.0	6.291159158459117	0.0
59	0.0	0.0	0.0	6.745731103211143	0.0
60	0.0	0.0	0.0	7.223614429745323	0.0
61	0.0	0.0	0.0	7.640305379101346	0.0
62	0.0	0.0	0.0	8.10070516929891	0.0
63	0.0	0.0	0.0	8.552363191328165	0.0
64	0.0	0.0	0.0	8.995279445189114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCATCT	15	0.0022064976	70.0	56
TTTCGTT	15	0.0022064976	70.0	18
CGCGGCC	20	0.0068949624	52.5	63
CCCTAGA	20	0.0068949624	52.5	2
ATAGACC	20	0.0068949624	52.5	4
GGGGGAA	20	0.0068949624	52.5	64
GCCAACC	20	0.0068949624	52.5	12
GACAAAA	35	0.0012388163	40.000004	36
>>END_MODULE