FastQCFastQC Report
Wed 25 May 2016
SRR1781129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594292
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGT200.006950394352.49999653
CGCGGTA352.0862488E-550.043
ACCCGAT404.592116E-543.74999627
GGTCGAT501.7094912E-435.00000410
TTAACGG501.7094912E-435.00000435
TTTAACG552.9917283E-431.8181834
TAACCGT552.9917283E-431.8181853
GTCGATG450.00430680331.11111311
CGACACT450.00430680331.11111345
CCGTGCA604.98143E-429.16666656
TAACGGC604.98143E-429.16666636
TAGCTCG604.98143E-429.16666636
GCGGTAC700.001225857825.044
CGTAACA750.001831994323.33333423
CGGTACC750.001831994323.33333445
TAGGACC1202.1660107E-623.3333324
CGAACCG800.002665646621.87499869
ACGGCCG850.003788641220.58823638
CACCCGA850.003788641220.58823626
GGTATAC1558.7346416E-720.322581
Produced by FastQC (version 0.11.5)