##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781129_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594292
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50688550409563	34.0	33.0	34.0	23.0	34.0
2	31.705261050123507	34.0	34.0	34.0	24.0	34.0
3	31.66707948281316	34.0	34.0	34.0	23.0	34.0
4	31.854361155795466	34.0	34.0	34.0	27.0	34.0
5	31.881411831221016	34.0	34.0	34.0	27.0	34.0
6	34.789446938542	38.0	35.0	38.0	27.0	38.0
7	34.70178464458549	38.0	35.0	38.0	27.0	38.0
8	34.599022029574684	38.0	35.0	38.0	26.0	38.0
9	34.799998990395295	38.0	35.0	38.0	27.0	38.0
10-11	34.68326260491476	38.0	35.0	38.0	26.5	38.0
12-13	34.61348714100139	38.0	35.0	38.0	26.0	38.0
14-15	34.49709233844642	38.0	34.5	38.0	26.0	38.0
16-17	34.466402038055364	38.0	34.0	38.0	25.5	38.0
18-19	34.53130531792452	38.0	34.5	38.0	26.0	38.0
20-21	34.45876017176741	38.0	34.0	38.0	25.5	38.0
22-23	34.520284641220144	38.0	34.5	38.0	25.5	38.0
24-25	34.506624689546555	38.0	34.5	38.0	26.0	38.0
26-27	34.43366476412269	38.0	34.0	38.0	25.5	38.0
28-29	34.52585176983705	38.0	34.0	38.0	26.0	38.0
30-31	34.598750277641294	38.0	35.0	38.0	26.0	38.0
32-33	34.55669098692225	38.0	34.5	38.0	26.0	38.0
34-35	34.58601074892477	38.0	35.0	38.0	26.0	38.0
36-37	34.44791028652581	37.5	34.0	38.0	25.5	38.0
38-39	34.46332021968999	38.0	34.0	38.0	25.5	38.0
40-41	34.46596959070625	37.5	34.0	38.0	25.5	38.0
42-43	34.54468510429217	38.0	34.5	38.0	26.0	38.0
44-45	34.55328525371367	38.0	34.5	38.0	26.0	38.0
46-47	34.5473706528104	38.0	34.5	38.0	25.5	38.0
48-49	34.512342417532125	37.5	34.5	38.0	25.5	38.0
50-51	34.40215079455891	37.0	34.0	38.0	24.5	38.0
52-53	34.48026727601919	37.0	34.0	38.0	25.5	38.0
54-55	34.43536090002894	37.0	34.0	38.0	25.0	38.0
56-57	34.40398490977499	37.0	34.0	38.0	25.0	38.0
58-59	34.393846795851196	37.0	34.0	38.0	25.0	38.0
60-61	34.39384090649041	37.0	34.0	38.0	25.0	38.0
62-63	34.365434836746914	37.0	34.0	38.0	24.5	38.0
64-65	34.37371191266246	37.0	34.0	38.0	25.0	38.0
66-67	34.27167705437731	37.0	34.0	38.0	24.0	38.0
68-69	34.05019670465024	37.0	34.0	38.0	23.5	38.0
70-71	34.22903808228952	37.0	34.0	38.0	24.0	38.0
72-73	34.19230445639518	37.0	34.0	38.0	24.0	38.0
74-75	34.19759478505516	37.0	34.0	38.0	24.0	38.0
76	33.03793757950637	37.0	32.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	37.0
14	129.0
15	390.0
16	856.0
17	1537.0
18	2307.0
19	2798.0
20	3220.0
21	3824.0
22	4469.0
23	5505.0
24	6388.0
25	8005.0
26	9287.0
27	10931.0
28	13154.0
29	15746.0
30	18645.0
31	22757.0
32	27805.0
33	34088.0
34	43516.0
35	59026.0
36	90713.0
37	209153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.513693605163795	18.28427776244674	8.955193743143102	24.24683488924636
2	22.742187342249267	22.16637612486791	31.66389586263991	23.427540670242912
3	19.39029971798375	23.007040310150565	31.25214541000047	26.35051456186521
4	13.478222826489336	17.726134627422212	38.199235392702576	30.59640715338588
5	13.047289884433916	33.93836699804137	37.03751691087883	15.976826206645892
6	27.85499384141129	38.68990327986916	20.083393348724197	13.371709529995357
7	26.8024809352978	31.615771371648954	23.00603070544446	18.575716987608786
8	24.402482281437408	36.377740235439816	21.122781393658336	18.096996089464437
9	24.155633930794963	17.68188028780465	22.133059169566476	36.02942661183391
10-11	23.7281504714854	25.57362374051813	29.177155337780082	21.521070450216392
12-13	24.497805792438733	23.92535992407773	28.27288605601287	23.303948227470674
14-15	22.504930236314806	25.00260814549077	25.41318072597309	27.07928089222133
16-17	22.425507999434622	28.50803981880961	25.907045694708998	23.15940648704677
18-19	21.538317863945668	28.089306266952946	27.996843302618913	22.37553256648247
20-21	21.826307606361855	28.308727023079566	27.897902041420714	21.967063329137865
22-23	21.778098981645385	27.995581296736283	28.102851796759843	22.12346792485849
24-25	21.895886197357527	27.939800636724033	27.801653059438795	22.36266010647964
26-27	21.718532303985246	27.815619257873237	27.731990334717615	22.733858103423906
28-29	21.638184596124464	27.812338042578393	28.21584339011799	22.33363397117915
30-31	21.650720521225256	28.111181035585204	28.10781568656485	22.130282756624688
32-33	21.319233642721088	28.329423919554696	28.313017843080505	22.03832459464371
34-35	21.622703990630868	27.82849171787606	28.453184629777954	22.095619661715116
36-37	21.94645056638824	27.918514804170343	27.835390683367773	22.299643946073648
38-39	21.59594946591911	28.128091914412444	28.131962065785842	22.1439965538826
40-41	21.48750109373843	27.68033222725529	28.66461268198125	22.16755399702503
42-43	21.323860997624063	27.78087202923815	28.588050991768355	22.307215981369428
44-45	21.298200211343918	27.667207366075935	28.65064648354681	22.383945939033335
46-47	21.3019020952663	27.604780141748503	28.669744839237275	22.423572923747923
48-49	20.952999535581835	27.598049443707808	28.917097992232776	22.531853028477585
50-51	21.10368303796787	27.50659608407988	28.858709186729758	22.531011691222496
52-53	20.78111433436762	27.434830016220985	29.16756409307209	22.61649155633931
54-55	20.623700133940893	27.5407543766364	29.144595586008226	22.690949903414484
56-57	20.66896407826456	27.40900096248982	29.07897128011146	22.84306367913416
58-59	20.628495756294885	27.3273071150209	29.12145881149334	22.922738317190877
60-61	20.635478855512105	27.52140361976941	28.792075276126887	23.051042248591603
62-63	20.372476829571994	27.452582232303314	29.026303567943028	23.148637370181664
64-65	20.63514232061007	27.17182799028087	29.029416515786856	23.163613173322204
66-67	20.527871820586512	27.062117612217563	28.837255086725044	23.57275548047088
68-69	20.655923350810713	27.07869195614277	28.4516702227188	23.813714470327717
70-71	21.157865157195452	27.06413682162977	28.239484966985927	23.53851305418885
72-73	20.729372093179784	26.898561649828707	28.43122572742019	23.94084052957132
74-75	20.892928055568643	26.607627227019716	28.432824268204854	24.066620449206788
76	21.143814825035502	26.449119288161377	28.03504001399985	24.372025872803267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	2.5
10	2.5
11	5.5
12	10.0
13	26.0
14	60.5
15	115.5
16	247.0
17	342.0
18	471.5
19	895.0
20	1525.5
21	1862.0
22	2333.5
23	3508.0
24	4780.5
25	5350.0
26	6277.0
27	8104.0
28	10151.0
29	11298.0
30	12486.0
31	14508.0
32	16576.5
33	17811.0
34	19017.5
35	21468.0
36	23913.5
37	25115.0
38	26084.0
39	27921.0
40	29214.0
41	29992.5
42	30346.0
43	30440.5
44	29985.0
45	28984.5
46	28534.0
47	27710.5
48	25813.0
49	23808.5
50	22878.0
51	21509.0
52	18899.5
53	16719.5
54	15780.0
55	15366.5
56	14073.5
57	12173.0
58	11152.0
59	10241.0
60	8555.0
61	7143.0
62	6506.0
63	5975.5
64	5011.0
65	4333.5
66	3861.5
67	3633.0
68	3353.0
69	2988.0
70	2784.0
71	2665.0
72	2475.5
73	2180.5
74	1986.0
75	1897.0
76	1803.0
77	1543.5
78	1240.5
79	1103.0
80	1013.5
81	815.5
82	608.5
83	510.0
84	428.5
85	285.5
86	185.0
87	146.0
88	111.0
89	73.5
90	50.0
91	22.5
92	16.0
93	12.5
94	9.5
95	9.0
96	8.0
97	6.0
98	4.0
99	7.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	594292.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.3266765199712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67040466333054	44.75321855650198
2	18.153601006819926	22.992144372630182
3	6.6266181707280145	12.58925115957169
4	2.2685239638646597	5.746323329498406
5	0.7753954431797052	2.4551608202650446
6	0.3585071314445091	1.3621839085853533
7	0.19951991019389767	0.8844452968499864
8	0.14770316634477493	0.7482840508872849
9	0.11740430508591414	0.6691342005204926
>10	0.670376852535089	7.326645850335144
>50	0.011945386472957327	0.47320845435437203
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.04802353052035E-4	0.0
2	0.0	0.0	0.0	5.04802353052035E-4	0.0
3	0.0	0.0	0.0	5.04802353052035E-4	0.0
4	0.0	0.0	0.0	0.001177872157121415	0.0
5	0.0	0.0	0.0	0.0018509419611907952	0.0
6	0.0	0.0	0.0	0.0018509419611907952	0.0
7	0.0	0.0	0.0	0.0018509419611907952	0.0
8	0.0	0.0	0.0	0.00201920941220814	0.0
9	0.0	0.0	0.0	0.0031970815693295553	0.0
10	0.0	0.0	0.0	0.0033653490203469	0.0
11	0.0	0.0	0.0	0.003870151373398935	0.0
12	0.0	0.0	0.0	0.004206686275433625	0.0
13	0.0	0.0	0.0	0.0043749537264509706	0.0
14	0.0	0.0	0.0	0.0043749537264509706	0.0
15	0.0	0.0	0.0	0.004543221177468315	0.0
16	0.0	0.0	0.0	0.004543221177468315	0.0
17	0.0	0.0	0.0	0.004879756079503006	0.0
18	0.0	0.0	0.0	0.00504802353052035	0.0
19	0.0	0.0	0.0	0.005216290981537695	0.0
20	0.0	0.0	0.0	0.005552825883572385	0.0
21	0.0	0.0	0.0	0.00605762823662442	0.0
22	0.0	0.0	0.0	0.006394163138659111	0.0
23	0.0	0.0	0.0	0.006562430589676455	0.0
24	0.0	0.0	0.0	0.00841337255086725	0.0
25	0.0	0.0	0.0	0.008918174903919286	0.0
26	0.0	0.0	0.0	0.00942297725697132	0.0
27	0.0	0.0	0.0	0.011946989022231496	0.0
28	0.0	0.0	0.0	0.017331547454786538	0.0
29	0.0	0.0	0.0	0.028773734123965996	0.0
30	0.0	0.0	0.0	0.039879385891110765	0.0
31	0.0	0.0	0.0	0.05283597961944633	0.0
32	0.0	0.0	0.0	0.06831658511304207	0.0
33	0.0	0.0	0.0	0.08346065570460312	0.0
34	0.0	0.0	0.0	0.11341226198569053	0.0
35	0.0	0.0	0.0	0.14942149650340236	0.0
36	0.0	0.0	0.0	0.21168045337982003	0.0
37	0.0	0.0	0.0	0.30691983065563727	0.0
38	0.0	0.0	0.0	0.456509594610057	0.0
39	0.0	0.0	0.0	0.666675640930721	0.0
40	0.0	0.0	0.0	0.9066250260814549	0.0
41	0.0	0.0	0.0	1.1425359924077727	0.0
42	0.0	0.0	0.0	1.3693605163791538	0.0
43	0.0	0.0	0.0	1.6044301454503846	0.0
44	0.0	0.0	0.0	1.8595236011926797	0.0
45	0.0	0.0	0.0	2.15735698949338	0.0
46	0.0	0.0	0.0	2.509709031923701	0.0
47	0.0	0.0	0.0	2.8793926218088077	0.0
48	0.0	0.0	0.0	3.2990516446460663	0.0
49	0.0	0.0	0.0	3.7673399608273375	0.0
50	0.0	0.0	0.0	4.220820741319082	0.0
51	0.0	0.0	0.0	4.6583161139641796	0.0
52	0.0	0.0	0.0	5.046172588559159	0.0
53	0.0	0.0	0.0	5.410303352560694	0.0
54	0.0	0.0	0.0	5.760467918127789	0.0
55	0.0	0.0	0.0	6.128805368404757	0.0
56	0.0	0.0	0.0	6.500844702604107	0.0
57	0.0	0.0	0.0	6.88600889798281	0.0
58	0.0	0.0	0.0	7.3147543631750045	0.0
59	0.0	0.0	0.0	7.740639281699905	1.6826745101734502E-4
60	0.0	0.0	0.0	8.199168085722171	1.6826745101734502E-4
61	0.0	0.0	0.0	8.636326923465232	1.6826745101734502E-4
62	0.0	0.0	0.0	9.066923330618618	1.6826745101734502E-4
63	0.0	0.0	0.0	9.443337618544419	1.6826745101734502E-4
64	0.0	0.0	0.0	9.812852940978509	1.6826745101734502E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGGT	20	0.0069503943	52.499996	53
CGCGGTA	35	2.0862488E-5	50.0	43
ACCCGAT	40	4.592116E-5	43.749996	27
GGTCGAT	50	1.7094912E-4	35.000004	10
TTAACGG	50	1.7094912E-4	35.000004	35
TTTAACG	55	2.9917283E-4	31.81818	34
TAACCGT	55	2.9917283E-4	31.81818	53
GTCGATG	45	0.004306803	31.111113	11
CGACACT	45	0.004306803	31.111113	45
CCGTGCA	60	4.98143E-4	29.166666	56
TAACGGC	60	4.98143E-4	29.166666	36
TAGCTCG	60	4.98143E-4	29.166666	36
GCGGTAC	70	0.0012258578	25.0	44
CGTAACA	75	0.0018319943	23.333334	23
CGGTACC	75	0.0018319943	23.333334	45
TAGGACC	120	2.1660107E-6	23.333332	4
CGAACCG	80	0.0026656466	21.874998	69
ACGGCCG	85	0.0037886412	20.588236	38
CACCCGA	85	0.0037886412	20.588236	26
GGTATAC	155	8.7346416E-7	20.32258	1
>>END_MODULE