FastQCFastQC Report
Wed 25 May 2016
SRR1781129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538172
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT34130.6341838668678416No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGA150.002224259570.043
TAACGGC200.006950039452.49999636
CAATACG407.476847E-752.49999669
TCGTATG6300.051.66666840
CGTCTTC6350.050.70866448
CTCGTAT6300.050.55555739
CGTATGC6450.050.4651241
CCGTCTT6550.050.2290147
TATGCCG6500.050.07692343
TCTCGTA5700.049.7368438
ATGCCGT6700.048.58209244
AATCTCG5850.048.46153636
GCCGTCT6800.048.38235546
ATCTCGT6050.048.01652537
GTATGCC6850.047.5182542
TGCCGTC7000.047.045
GCCGACT350.001255173640.00000448
GTCTTCT8500.037.4705949
CTGCTTG8400.035.83333254
CAATCTT9150.035.57377229
Produced by FastQC (version 0.11.5)