FastQCFastQC Report
Wed 25 May 2016
SRR1781128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218757
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG200.00694455252.545
GATCATA200.00694455252.51
ATTTGCG305.868912E-446.66666431
CGACGAA757.512426E-1042.070
ACTATCG350.001253541440.017
TTTTACG459.177487E-538.8888950
GATTTGC501.7059683E-435.00000430
CGTAGTG501.7059683E-435.00000454
GGTACCC400.002415167935.016
GGCAAGC400.002415167935.013
TTGCGTT400.002415167935.033
GGGCATA400.002415167935.047
GCCGTTT400.002415167935.012
TCTAGCA751.4845336E-632.6666683
AGCCTCG552.9855943E-431.8181810
TTTAGAA450.004300912431.11111332
AAAGTCC450.004300912431.11111329
ACGATTC450.004300912431.11111365
GGCGCTA450.004300912431.1111138
GCGCTAT450.004300912431.1111139