##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781128_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	218757
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.500249134884825	34.0	27.0	34.0	12.0	34.0
2	29.66476501323386	34.0	27.0	34.0	12.0	34.0
3	29.572635389953234	34.0	30.0	34.0	11.0	34.0
4	29.901859140507504	34.0	31.0	34.0	11.0	34.0
5	29.967767888570425	34.0	31.0	34.0	11.0	34.0
6	32.32966716493644	37.0	31.0	38.0	11.0	38.0
7	32.09593750142852	37.0	31.0	38.0	12.0	38.0
8	31.790356422880183	36.0	28.0	38.0	11.0	38.0
9	32.321909698889634	37.0	31.0	38.0	12.0	38.0
10-11	32.03540686698026	37.0	31.0	38.0	11.0	38.0
12-13	32.00257591757064	37.0	31.0	38.0	11.0	38.0
14-15	31.895413632478046	36.5	31.0	38.0	11.0	38.0
16-17	31.835228129842704	36.5	29.5	38.0	11.0	38.0
18-19	31.914357026289444	37.0	31.0	38.0	11.0	38.0
20-21	31.818485808454128	36.5	29.5	38.0	11.0	38.0
22-23	31.920816248165774	37.0	31.0	38.0	11.0	38.0
24-25	31.923351024195796	36.5	31.0	38.0	11.0	38.0
26-27	31.824156484135365	36.0	31.0	38.0	11.0	38.0
28-29	31.930095951215275	37.0	31.0	38.0	11.0	38.0
30-31	32.00162966213652	37.0	31.0	38.0	11.0	38.0
32-33	31.970622654360774	37.0	31.0	38.0	11.0	38.0
34-35	31.976670460830967	37.0	31.0	38.0	11.0	38.0
36-37	31.809416384389984	36.0	29.5	38.0	11.0	38.0
38-39	31.805517080596278	36.0	30.5	38.0	11.0	38.0
40-41	31.78130071266291	36.0	29.0	38.0	11.0	38.0
42-43	31.868751628519316	36.5	31.0	38.0	11.0	38.0
44-45	31.880392398871805	36.5	31.0	38.0	11.0	38.0
46-47	31.873969747253803	36.5	30.5	38.0	11.0	38.0
48-49	31.83902229414373	36.0	30.5	38.0	11.0	38.0
50-51	31.659370900131194	36.0	27.5	38.0	11.0	38.0
52-53	31.763888698418796	36.0	28.5	38.0	11.0	38.0
54-55	31.709264160689713	36.0	27.5	38.0	11.0	38.0
56-57	31.699403904789335	36.0	27.5	38.0	11.0	38.0
58-59	31.682693582376793	36.0	27.5	38.0	11.0	38.0
60-61	31.644841079371176	36.0	27.0	38.0	11.0	38.0
62-63	31.618601919024307	36.0	27.0	38.0	11.0	38.0
64-65	31.640224084257873	36.0	27.0	38.0	11.0	38.0
66-67	31.50157937803133	36.0	27.0	38.0	11.0	38.0
68-69	31.257644326810112	36.0	27.0	38.0	11.0	38.0
70-71	31.4230881754641	36.0	27.0	38.0	11.0	38.0
72-73	31.371279547625903	36.0	27.0	38.0	11.0	38.0
74-75	31.358571382858607	36.0	27.0	38.0	11.0	38.0
76	29.865718582719637	34.0	24.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	25.0
14	95.0
15	250.0
16	719.0
17	1538.0
18	2324.0
19	3259.0
20	3940.0
21	4333.0
22	4874.0
23	5583.0
24	5968.0
25	6694.0
26	7361.0
27	8037.0
28	8615.0
29	9427.0
30	10230.0
31	11264.0
32	12350.0
33	13765.0
34	15929.0
35	19026.0
36	25577.0
37	37571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.511622485223334	18.127419922562478	9.101423040177	25.259534552037195
2	24.324707323651356	20.313407113829502	30.531137289321027	24.830748273198115
3	19.485547891038916	22.453681482192568	29.420772820984016	28.6399978057845
4	13.657162970784936	16.59375471413486	37.94941419017449	31.79966812490572
5	13.286431977033878	33.986112444401776	36.63014212116641	16.097313457397934
6	29.10581147117578	38.40425677806882	18.379297576763257	14.110634173992146
7	28.782621813244834	29.179866244280184	22.141462901758572	19.896049040716413
8	25.67003570171469	33.832517359444495	19.26841198224514	21.229034956595676
9	24.76492180821642	16.03514401824856	20.968928994272183	38.23100517926284
10-11	25.090625671407086	24.193740086031532	27.811910018879395	22.903724223681984
12-13	25.239420909959453	22.913095352377294	26.477781282427532	25.36970245523572
14-15	23.508733434815802	23.82529473342567	24.004488999209165	28.661482832549357
16-17	23.908263506996345	26.68806026778572	25.01977079590596	24.383905429311977
18-19	23.064633360303898	26.41286907390392	26.333557326165565	24.188940239626618
20-21	23.106003465031975	26.3582422505337	26.41424045859104	24.121513825843287
22-23	23.607016004059297	25.811974016831464	26.629547854468655	23.95146212464058
24-25	23.115374593727285	26.439153947073695	26.42429727962991	24.021174179569112
26-27	22.59653405376742	26.343157018975393	26.835255557536446	24.22505336972074
28-29	23.127259927682314	26.3200720434089	27.363238662077098	23.18942936683169
30-31	23.43102163587908	26.178362292406643	26.795028273380968	23.595587798333312
32-33	22.670588826871825	26.646461598943123	26.693317242419674	23.989632331765385
34-35	23.74095457516788	25.585466979342375	27.049877261070502	23.62370118441924
36-37	23.75169708855031	25.72557678154299	26.31595788934754	24.20676824055916
38-39	23.0612048985861	26.453782050402957	27.029992183107282	23.455020867903656
40-41	23.3178823991918	25.307533016086342	27.491920258551726	23.882664326170133
42-43	22.892296017955996	25.91825633008315	27.080961980645192	24.10848567131566
44-45	22.37436973445421	25.820888017297733	27.174673267598294	24.63006898064976
46-47	22.78281380710103	25.246506397509567	27.39638045868247	24.57429933670694
48-49	22.190147058151283	25.313704247178375	28.044588287460513	24.451560407209826
50-51	22.455967123337768	25.20285065163629	27.588602878993584	24.752579346032356
52-53	22.283401216875344	25.014056693042964	28.07018746828673	24.63235462179496
54-55	21.546281947549105	25.604209236732995	28.184926653775648	24.664582161942246
56-57	21.29130172429556	25.62284005924409	28.233968439722794	24.85188977673755
58-59	21.712090182669275	25.117939622227503	27.808151547843256	25.36181864725996
60-61	21.13519172045567	25.22170820457496	28.11602881749529	25.52707125747408
62-63	20.632805500191996	24.900574155680303	28.700698495126993	25.765921849000712
64-65	21.365585401086143	25.078169284499623	28.01934575508786	25.536899559326372
66-67	21.37518513777908	24.90285980727386	27.54461591910622	26.177339135840842
68-69	20.914169211358775	25.17828082429739	27.34210718791713	26.565442776426707
70-71	21.683241412255178	24.881089247633785	27.443298827691976	25.99237051241906
72-73	21.774895831666715	24.262593340083612	26.97588414515683	26.986626683092847
74-75	21.573579696099763	24.54355537676681	27.014573314560515	26.868291612572914
76	21.927069762339034	24.19442577837509	26.718687859131364	27.159816600154507
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	2.5
15	5.0
16	12.0
17	18.0
18	28.5
19	44.0
20	61.5
21	74.0
22	113.5
23	213.0
24	330.5
25	388.0
26	515.0
27	765.0
28	1028.0
29	1168.0
30	1404.5
31	1882.5
32	2659.0
33	3194.0
34	3604.5
35	4688.5
36	6061.0
37	6760.0
38	7517.5
39	9320.5
40	11289.5
41	12801.0
42	13389.0
43	13731.0
44	14079.5
45	14279.5
46	14473.0
47	14182.0
48	13220.0
49	12047.0
50	11545.0
51	10653.0
52	8964.0
53	7577.5
54	6988.0
55	6384.5
56	5287.0
57	4325.5
58	3858.0
59	3478.5
60	2812.5
61	2383.0
62	2240.0
63	2165.0
64	1995.0
65	1833.0
66	1767.0
67	1768.0
68	1696.0
69	1577.0
70	1502.0
71	1474.0
72	1447.0
73	1326.5
74	1150.0
75	1067.0
76	1020.0
77	877.0
78	714.0
79	647.0
80	578.0
81	433.5
82	323.0
83	288.0
84	227.0
85	134.5
86	88.0
87	73.0
88	60.0
89	34.0
90	20.0
91	14.0
92	9.0
93	7.5
94	5.0
95	3.0
96	2.0
97	2.5
98	1.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.5712822903952785E-4
58-59	4.5712822903952785E-4
60-61	4.5712822903952785E-4
62-63	4.5712822903952785E-4
64-65	4.5712822903952785E-4
66-67	4.5712822903952785E-4
68-69	4.5712822903952785E-4
70-71	2.2856411451976393E-4
72-73	2.2856411451976393E-4
74-75	4.5712822903952785E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	218757.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.59624417513443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97206082114022	41.010375497871244
2	10.00934577213907	10.529079885295522
3	3.933689143262812	6.206918240643787
4	1.8821938056482888	3.9598529994721168
5	1.1592637269636066	3.048645902337711
6	0.7640505991528195	2.411171512511967
7	0.6407447787612428	2.3590538186368173
8	0.5155555786337154	2.169302967973729
9	0.3774744091293217	1.7868362573187357
>10	2.6949604853324867	24.809779578402445
>50	0.05066087983639707	1.708983339535975
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0018285129161581114	0.0
5	0.0	0.0	0.0	0.0018285129161581114	0.0
6	0.0	0.0	0.0	0.0018285129161581114	0.0
7	0.0	0.0	0.0	0.0018285129161581114	0.0
8	0.0	0.0	0.0	0.0018285129161581114	0.0
9	0.0	0.0	0.0	0.0018285129161581114	0.0
10	0.0	0.0	0.0	0.0018285129161581114	0.0
11	0.0	0.0	0.0	0.0031998976032766953	4.5712822903952785E-4
12	0.0	0.0	0.0	0.0031998976032766953	4.5712822903952785E-4
13	0.0	0.0	0.0	0.0031998976032766953	4.5712822903952785E-4
14	0.0	0.0	0.0	0.003657025832316223	4.5712822903952785E-4
15	0.0	0.0	0.0	0.004114154061355751	4.5712822903952785E-4
16	0.0	0.0	0.0	0.004571282290395278	4.5712822903952785E-4
17	0.0	0.0	0.0	0.004571282290395278	4.5712822903952785E-4
18	0.0	0.0	0.0	0.0050284105194348064	4.5712822903952785E-4
19	0.0	0.0	0.0	0.0054855387484743345	4.5712822903952785E-4
20	0.0	0.0	0.0	0.0063997952065533905	4.5712822903952785E-4
21	0.0	0.0	0.0	0.0068569234355929185	4.5712822903952785E-4
22	0.0	0.0	0.0	0.008228308122711503	4.5712822903952785E-4
23	0.0	0.0	0.0	0.009142564580790557	4.5712822903952785E-4
24	0.0	0.0	0.0	0.010971077496948669	4.5712822903952785E-4
25	0.0	0.0	0.0	0.011885333955027725	4.5712822903952785E-4
26	0.0	0.0	0.0	0.012799590413106781	4.5712822903952785E-4
27	0.0	0.0	0.0	0.020113642077739226	4.5712822903952785E-4
28	0.0	0.0	0.0	0.026513437284292618	4.5712822903952785E-4
29	0.0	0.0	0.0	0.033827488948925066	4.5712822903952785E-4
30	0.0	0.0	0.0	0.04022728415547845	4.5712822903952785E-4
31	0.0	0.0	0.0	0.05211261811050618	4.5712822903952785E-4
32	0.0	0.0	0.0	0.06354082383649437	4.5712822903952785E-4
33	0.0	0.0	0.0	0.07862605539479879	4.5712822903952785E-4
34	0.0	0.0	0.0	0.11153928788564481	4.5712822903952785E-4
35	0.0	0.0	0.0	0.16456616245423003	4.5712822903952785E-4
36	0.0	0.0	0.0	0.23907806378767307	4.5712822903952785E-4
37	0.0	0.0	0.0	0.3625026856283456	4.5712822903952785E-4
38	0.0	0.0	0.0	0.5252403351664175	4.5712822903952785E-4
39	0.0	0.0	0.0	0.7236339865695727	4.5712822903952785E-4
40	0.0	0.0	0.0	0.9471696905699017	4.5712822903952785E-4
41	0.0	0.0	0.0	1.2004187294578001	4.5712822903952785E-4
42	0.0	0.0	0.0	1.4847524879203866	4.5712822903952785E-4
43	0.0	0.0	0.0	1.8399411218840998	4.5712822903952785E-4
44	0.0	0.0	0.0	2.262784733745663	4.5712822903952785E-4
45	0.0	0.0	0.0	2.781168145476488	4.5712822903952785E-4
46	0.0	0.0	0.0	3.360806739898609	4.5712822903952785E-4
47	0.0	0.0	0.0	4.014957235654173	4.5712822903952785E-4
48	0.0	0.0	0.0	4.654936756309512	4.5712822903952785E-4
49	0.0	0.0	0.0	5.201662118240788	4.5712822903952785E-4
50	0.0	0.0	0.0	5.71273147830698	4.5712822903952785E-4
51	0.0	0.0	0.0	6.199115914005038	4.5712822903952785E-4
52	0.0	0.0	0.0	6.685957477932135	4.5712822903952785E-4
53	0.0	0.0	0.0	7.152228271552453	4.5712822903952785E-4
54	0.0	0.0	0.0	7.722724301393784	4.5712822903952785E-4
55	0.0	0.0	0.0	8.358132539758728	4.5712822903952785E-4
56	0.0	0.0	0.0	8.99399790635271	4.5712822903952785E-4
57	0.0	0.0	0.0	9.64357711981788	4.5712822903952785E-4
58	0.0	0.0	0.0	10.277613973495706	4.5712822903952785E-4
59	0.0	0.0	0.0	10.909365186028333	4.5712822903952785E-4
60	0.0	0.0	0.0	11.536087988041526	4.5712822903952785E-4
61	0.0	0.0	0.0	12.104755504966699	4.5712822903952785E-4
62	0.0	0.0	0.0	12.678908560640345	4.5712822903952785E-4
63	0.0	0.0	0.0	13.228376691945858	4.5712822903952785E-4
64	0.0	0.0	0.0	13.798415593558149	4.5712822903952785E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	0.006944552	52.5	45
GATCATA	20	0.006944552	52.5	1
ATTTGCG	30	5.868912E-4	46.666664	31
CGACGAA	75	7.512426E-10	42.0	70
ACTATCG	35	0.0012535414	40.0	17
TTTTACG	45	9.177487E-5	38.88889	50
GATTTGC	50	1.7059683E-4	35.000004	30
CGTAGTG	50	1.7059683E-4	35.000004	54
GGTACCC	40	0.0024151679	35.0	16
GGCAAGC	40	0.0024151679	35.0	13
TTGCGTT	40	0.0024151679	35.0	33
GGGCATA	40	0.0024151679	35.0	47
GCCGTTT	40	0.0024151679	35.0	12
TCTAGCA	75	1.4845336E-6	32.666668	3
AGCCTCG	55	2.9855943E-4	31.81818	10
TTTAGAA	45	0.0043009124	31.111113	32
AAAGTCC	45	0.0043009124	31.111113	29
ACGATTC	45	0.0043009124	31.111113	65
GGCGCTA	45	0.0043009124	31.111113	8
GCGCTAT	45	0.0043009124	31.111113	9
>>END_MODULE