FastQCFastQC Report
Wed 25 May 2016
SRR1781128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197576
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT26941.363525934324007No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG2650.13412560229987447No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC2170.10983115358140666No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAG150.002222170470.0000146
TATGCCG3950.059.36709243
TCGTATG3900.059.2307740
CGTATGC3950.058.48101441
CTCGTAT3900.058.33333239
TCTCGTA3750.057.8666738
GCCGTCT4000.057.74999646
ATCTCGT3800.057.1052637
GATAAGC252.386177E-455.99999653
GTAGACA252.386177E-455.9999964
ATGCCGT4150.055.6626544
TGCCGTC4150.055.6626545
CCGTCTT4100.055.48780447
TCTTCTG4000.054.24999650
CTGCTTG4000.053.37499654
CGCACCG200.00694356152.510
CGTCTTC4350.052.2988548
TATCTCG4150.052.28915836
CATTTAT4200.051.66666432
TTATCTC4150.051.44578635
Produced by FastQC (version 0.11.5)