##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781128_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197576
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.31308964651577	34.0	34.0	34.0	33.0	34.0
2	33.304470178564195	34.0	34.0	34.0	33.0	34.0
3	33.35308944406203	34.0	34.0	34.0	33.0	34.0
4	33.423720492367494	34.0	34.0	34.0	34.0	34.0
5	33.40869842490991	34.0	34.0	34.0	34.0	34.0
6	36.850513220229175	38.0	37.0	38.0	34.0	38.0
7	36.75815382435113	38.0	37.0	38.0	34.0	38.0
8	36.56965927035672	38.0	37.0	38.0	34.0	38.0
9	36.5118890958416	38.0	37.0	38.0	34.0	38.0
10-11	36.41709519374823	38.0	37.0	38.0	34.0	38.0
12-13	36.348453253431586	38.0	37.0	38.0	34.0	38.0
14-15	36.36222263837713	38.0	37.0	38.0	34.0	38.0
16-17	36.31463588694983	38.0	37.0	38.0	34.0	38.0
18-19	36.31630106895574	38.0	37.0	38.0	34.0	38.0
20-21	36.24322286107625	38.0	37.0	38.0	34.0	38.0
22-23	36.31836862776856	38.0	37.0	38.0	34.0	38.0
24-25	36.298669878932664	38.0	37.0	38.0	34.0	38.0
26-27	36.07008948455278	38.0	37.0	38.0	33.0	38.0
28-29	36.07996416568814	38.0	37.0	38.0	33.0	38.0
30-31	36.0947711260477	38.0	37.0	38.0	32.5	38.0
32-33	36.050249018099365	38.0	37.0	38.0	32.0	38.0
34-35	36.058536968052806	38.0	37.0	38.0	32.5	38.0
36-37	36.01658602259384	38.0	37.0	38.0	32.0	38.0
38-39	35.95202605579625	38.0	37.0	38.0	32.0	38.0
40-41	36.01016317771389	38.0	37.0	38.0	32.0	38.0
42-43	36.008169008381586	38.0	37.0	38.0	32.0	38.0
44-45	35.99043912216059	38.0	37.0	38.0	32.0	38.0
46-47	36.00429201927359	38.0	37.0	38.0	32.0	38.0
48-49	35.99016327894076	38.0	37.0	38.0	32.0	38.0
50-51	35.88124569785804	38.0	36.5	38.0	31.5	38.0
52-53	35.908986921488435	38.0	37.0	38.0	32.0	38.0
54-55	35.922447564481516	38.0	37.0	38.0	32.0	38.0
56-57	35.88099769202737	38.0	37.0	38.0	32.0	38.0
58-59	35.87581993764425	38.0	37.0	38.0	31.5	38.0
60-61	35.861068652063004	38.0	36.5	38.0	31.5	38.0
62-63	35.841068753289875	38.0	36.5	38.0	31.0	38.0
64-65	35.796356844960926	38.0	36.0	38.0	31.0	38.0
66-67	35.773115155686924	38.0	36.0	38.0	31.0	38.0
68-69	35.704265700287486	38.0	36.0	38.0	31.0	38.0
70-71	35.5698895614852	38.0	36.0	38.0	31.0	38.0
72-73	35.46905494594485	38.0	36.0	38.0	31.0	38.0
74-75	35.47181590881483	38.0	36.0	38.0	31.0	38.0
76	34.58201907114224	37.0	34.0	38.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	6.0
22	27.0
23	52.0
24	125.0
25	269.0
26	483.0
27	977.0
28	1607.0
29	2513.0
30	3848.0
31	5804.0
32	8387.0
33	11698.0
34	16011.0
35	22422.0
36	34077.0
37	89265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26270397214237	17.781005790176945	9.261246305219258	26.69504393246143
2	24.26914200105276	20.04595699882577	30.866603231161683	24.818297768959795
3	19.778211928574322	22.1342673199174	29.574442239948173	28.51307851156011
4	13.659553791958537	17.798720492367494	37.015629428675545	31.526096286998424
5	13.126088188848847	34.338178726161075	37.034862533911	15.50087055107908
6	29.623537271733408	39.21022796291048	17.41760132809653	13.748633437259587
7	28.88964246669636	29.660991213507714	21.159452565088877	20.28991375470705
8	25.552698708345144	35.5296189820626	18.83325910029558	20.084423209296677
9	24.602684536583393	15.720026723893591	20.23018990160748	39.44709883791553
10-11	24.71732396647366	25.15437097623193	27.84700570919545	22.28129934809896
12-13	24.989624245859822	22.82514070534883	26.683402842450498	25.50183220634085
14-15	23.01620642183261	23.71745556140422	23.131098918897035	30.13523909786614
16-17	24.362776855488523	27.461078268615623	24.31266955500668	23.863475320889176
18-19	23.79388184799773	26.46196906506863	25.60154067295623	24.142608413977406
20-21	22.775286472041138	26.388326517390777	25.45121877151071	25.385168239057375
22-23	23.006083734866582	26.704660485079156	25.95507551524477	24.33418026480949
24-25	23.83639713325505	27.10298821719237	25.677207758027293	23.383406891525286
26-27	23.15969550957606	27.322397457181037	25.94672429849779	23.57118273474511
28-29	23.769081264930964	26.003664412681704	26.74059602380856	23.486658298578774
30-31	24.026956715390533	25.956087783941367	26.922298254848766	23.09465724581933
32-33	22.558914038142284	27.339606025023283	25.856632384500145	24.244847552334292
34-35	23.5137364862129	25.690873385431427	27.802212819370776	22.993177308984897
36-37	23.338867068874762	26.454377049844112	26.69504393246143	23.511711948819695
38-39	22.51715795440742	26.523464388387254	27.06755881281127	23.891818844394056
40-41	22.710501275458558	25.249524233712595	27.882688180750698	24.157286310078145
42-43	23.60180386281735	25.438312345629022	27.236607685143944	23.723276106409685
44-45	22.060877839413696	26.851692513260723	27.243693566020166	23.843736081305423
46-47	23.364679920638135	25.173857148641538	26.544975098190065	24.91648783253027
48-49	22.742387739401547	25.038466210470904	27.298103008462565	24.92104304166498
50-51	22.039367129610884	25.146019759484954	27.773616228691743	25.04099688221241
52-53	21.662803174474632	24.693535652103492	29.368192493015343	24.27546868040653
54-55	20.9359942503138	25.5190407741831	28.55027938616026	24.994685589342836
56-57	21.692412033850264	25.346702028586463	27.44943717860469	25.511448758958576
58-59	21.004322387334497	25.08654897355954	28.84333117382678	25.065797465279182
60-61	21.507926063894402	25.982659837227196	27.35479005547232	25.15462404340608
62-63	19.625865489735595	26.762865935133824	27.823723529173584	25.787545045957
64-65	20.389116086974127	26.99062639186946	27.002520549054545	25.617736972101874
66-67	20.403793983074866	26.904583552658217	26.514353970117828	26.177268494149086
68-69	20.411385998299387	27.43096327489169	25.840436085354497	26.317214641454427
70-71	20.90258938332591	26.925081993764426	25.844991294489212	26.32733732842046
72-73	20.8820909422197	25.69492246021784	25.763250597238528	27.659736000323925
74-75	20.890948293314977	25.58762197837794	26.017583107259988	27.50384662104709
76	21.44390006883427	25.59976920273718	25.822974450338098	27.133356278090453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.5
15	3.0
16	8.0
17	11.0
18	21.5
19	51.0
20	64.0
21	58.0
22	82.5
23	184.5
24	335.5
25	409.0
26	542.5
27	726.0
28	912.0
29	1048.0
30	1288.0
31	1664.5
32	2333.5
33	2866.0
34	3262.5
35	4108.5
36	5287.5
37	6017.0
38	6693.0
39	8419.5
40	10666.0
41	12370.5
42	12879.0
43	13186.0
44	13367.5
45	13167.0
46	13092.0
47	12805.5
48	11753.5
49	10678.5
50	10369.0
51	9418.0
52	7627.5
53	6441.0
54	6094.0
55	5597.0
56	4607.0
57	3728.0
58	3342.0
59	2927.5
60	2310.0
61	1982.5
62	1858.0
63	1799.0
64	1676.0
65	1580.0
66	1536.5
67	1525.0
68	1512.5
69	1479.0
70	1378.5
71	1299.0
72	1321.5
73	1262.0
74	1110.5
75	1041.0
76	984.5
77	858.5
78	709.0
79	629.0
80	569.0
81	430.0
82	290.5
83	230.0
84	187.5
85	121.5
86	70.0
87	42.0
88	35.0
89	23.0
90	12.0
91	5.0
92	4.0
93	2.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	197576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.15279183706523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.8623390777195	22.612058144713934
2	17.99332470540154	13.370044944730127
3	7.231115046658947	8.059683362351702
4	3.3608064845718952	4.994533749038345
5	1.9290239084531025	3.583431185974005
6	1.3500442749131532	3.0094748350002023
7	1.0571486955929432	2.749321779973276
8	0.7819630815339553	2.324168927400089
9	0.6770655949867175	2.263938939952221
>10	4.571895647435461	30.94151111471029
>50	0.1743750425720319	4.175102239138357
>100	0.009536135140657993	0.5532048426934446
>500	0.0	0.0
>1k	0.0013623050200939992	1.363525934324007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT	2694	1.363525934324007	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG	265	0.13412560229987447	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC	217	0.10983115358140666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010122686966028262	0.0
2	0.0	0.0	0.0	0.0010122686966028262	0.0
3	0.0	0.0	0.0	0.0010122686966028262	0.0
4	0.0	0.0	0.0	0.0025306717415070658	0.0
5	0.0	0.0	0.0	0.0025306717415070658	0.0
6	0.0	0.0	0.0	0.0025306717415070658	0.0
7	0.0	0.0	0.0	0.0025306717415070658	0.0
8	0.0	0.0	0.0	0.0025306717415070658	0.0
9	0.0	0.0	0.0	0.0025306717415070658	0.0
10	0.0	0.0	0.0	0.0025306717415070658	0.0
11	0.0	0.0	0.0	0.004049074786411305	0.0
12	0.0	0.0	0.0	0.004049074786411305	0.0
13	0.0	0.0	0.0	0.004555209134712718	0.0
14	0.0	0.0	0.0	0.004555209134712718	0.0
15	0.0	0.0	0.0	0.0055674778313155445	0.0
16	0.0	0.0	0.0	0.0065797465279183705	0.0
17	0.0	0.0	0.0	0.0065797465279183705	0.0
18	0.0	0.0	0.0	0.0070858808762197835	0.0
19	0.0	0.0	0.0	0.0075920152245211965	0.0
20	0.0	0.0	0.0	0.008604283921124023	0.0
21	0.0	0.0	0.0	0.009110418269425435	0.0
22	0.0	0.0	0.0	0.009110418269425435	0.0
23	0.0	0.0	0.0	0.011134955662631089	0.0
24	0.0	0.0	0.0	0.011641090010932501	0.0
25	0.0	0.0	0.0	0.012653358707535329	0.0
26	0.0	0.0	0.0	0.013665627404138155	0.0
27	0.0	0.0	0.0	0.025306717415070658	0.0
28	0.0	0.0	0.0	0.03542940438109892	0.0
29	0.0	0.0	0.0	0.046564360043730005	0.0
30	0.0	0.0	0.0	0.05820545005466251	0.0
31	0.0	0.0	0.0	0.07592015224521197	0.0
32	0.0	0.0	0.0	0.09110418269425437	0.0
33	0.0	0.0	0.0	0.1118556909746123	0.0
34	0.0	0.0	0.0	0.16145685710815078	0.0
35	0.0	0.0	0.0	0.23383406891525285	0.0
36	0.0	0.0	0.0	0.338097744665344	0.0
37	0.0	0.0	0.0	0.5167631696157428	0.0
38	0.0	0.0	0.0	0.7242782524193222	0.0
39	0.0	0.0	0.0	0.98139450135644	0.0
40	0.0	0.0	0.0	1.2658420051018342	0.0
41	0.0	0.0	0.0	1.5897679880147386	0.0
42	0.0	0.0	0.0	1.954184718791756	0.0
43	0.0	0.0	0.0	2.4076810948698224	0.0
44	0.0	0.0	0.0	2.9492448475523343	0.0
45	0.0	0.0	0.0	3.59962748511965	0.0
46	0.0	0.0	0.0	4.326942543628781	0.0
47	0.0	0.0	0.0	5.128153216989918	0.0
48	0.0	0.0	0.0	5.9116491881605056	0.0
49	0.0	0.0	0.0	6.586326274446289	0.0
50	0.0	0.0	0.0	7.218488075474754	0.0
51	0.0	0.0	0.0	7.821800218650038	0.0
52	0.0	0.0	0.0	8.437259586184556	0.0
53	0.0	0.0	0.0	9.024375430214196	0.0
54	0.0	0.0	0.0	9.721322427825243	0.0
55	0.0	0.0	0.0	10.490140502895088	0.0
56	0.0	0.0	0.0	11.263007652751346	0.0
57	0.0	0.0	0.0	12.094080252662266	0.0
58	0.0	0.0	0.0	12.870996477304935	0.0
59	0.0	0.0	0.0	13.661578329351743	0.0
60	0.0	0.0	0.0	14.415718508320849	0.0
61	0.0	0.0	0.0	15.098493744179455	0.0
62	0.0	0.0	0.0	15.768109486982224	0.0
63	0.0	0.0	0.0	16.41444304976313	0.0
64	0.0	0.0	0.0	17.08152812082439	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAG	15	0.0022221704	70.00001	46
TATGCCG	395	0.0	59.367092	43
TCGTATG	390	0.0	59.23077	40
CGTATGC	395	0.0	58.481014	41
CTCGTAT	390	0.0	58.333332	39
TCTCGTA	375	0.0	57.86667	38
GCCGTCT	400	0.0	57.749996	46
ATCTCGT	380	0.0	57.10526	37
GATAAGC	25	2.386177E-4	55.999996	53
GTAGACA	25	2.386177E-4	55.999996	4
ATGCCGT	415	0.0	55.66265	44
TGCCGTC	415	0.0	55.66265	45
CCGTCTT	410	0.0	55.487804	47
TCTTCTG	400	0.0	54.249996	50
CTGCTTG	400	0.0	53.374996	54
CGCACCG	20	0.006943561	52.5	10
CGTCTTC	435	0.0	52.29885	48
TATCTCG	415	0.0	52.289158	36
CATTTAT	420	0.0	51.666664	32
TTATCTC	415	0.0	51.445786	35
>>END_MODULE