FastQCFastQC Report
Wed 25 May 2016
SRR1781127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114714
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.002219787670.000011
CTCGCTT150.002219787670.0000145
CACGTGA150.002219787670.0000120
TGCTTAG200.00693617352.512
GTGTAAC200.00693617352.51
GCAATTT200.00693617352.554
CCCTATA501.7009205E-435.02
CTCGAAT400.002410395835.012
TATACTC400.002410395835.05
AACAGGT400.002410395835.034
AGAGCAA400.002410395835.069
CAGGTAA450.00429246531.1111134
ATAGCAC450.00429246531.1111136
TGGCAAT450.00429246531.11111327
CTAACCG450.00429246531.11111321
TCGCTTC450.00429246531.11111346
ACAATTA450.00429246531.11111335
CGAATAA450.00429246531.11111314
ACTCAGC450.00429246531.1111138
TGTTATC500.007183763727.9999982