##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781127_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	114714
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462585211918338	34.0	33.0	34.0	23.0	34.0
2	31.529769688093868	34.0	33.0	34.0	23.0	34.0
3	31.45293512561675	34.0	33.0	34.0	23.0	34.0
4	31.579414892689645	34.0	34.0	34.0	23.0	34.0
5	31.586414910124308	34.0	34.0	34.0	23.0	34.0
6	34.385785518768415	37.0	34.0	38.0	25.0	38.0
7	34.40485904074481	37.0	34.0	38.0	25.0	38.0
8	34.201858535139564	37.0	34.0	38.0	24.0	38.0
9	34.45514061056192	38.0	34.0	38.0	25.0	38.0
10-11	34.281526230451384	37.5	34.0	38.0	24.0	38.0
12-13	34.251643217044126	37.0	34.0	38.0	24.0	38.0
14-15	34.111002144463626	37.0	34.0	38.0	24.0	38.0
16-17	34.06584636574438	37.0	34.0	38.0	23.5	38.0
18-19	34.09688442561501	37.0	34.0	38.0	24.0	38.0
20-21	34.045866241260875	37.0	34.0	38.0	23.5	38.0
22-23	34.100127273044265	37.0	34.0	38.0	23.5	38.0
24-25	34.054064891817916	37.0	34.0	38.0	23.5	38.0
26-27	33.98553358787942	37.0	34.0	38.0	23.0	38.0
28-29	34.05643600606726	37.0	34.0	38.0	24.0	38.0
30-31	34.118860819080496	37.0	34.0	38.0	24.0	38.0
32-33	34.05236937078299	37.0	34.0	38.0	23.5	38.0
34-35	34.07904440608819	37.0	34.0	38.0	24.0	38.0
36-37	33.96347873842774	37.0	34.0	38.0	23.5	38.0
38-39	33.96401049566748	37.0	34.0	38.0	23.0	38.0
40-41	33.96368795439092	37.0	34.0	38.0	23.5	38.0
42-43	34.031992607702634	37.0	34.0	38.0	23.5	38.0
44-45	34.089740572205656	37.0	34.0	38.0	24.0	38.0
46-47	34.07089370085604	37.0	34.0	38.0	24.0	38.0
48-49	34.00893962375996	37.0	34.0	38.0	23.5	38.0
50-51	33.881806056802134	37.0	34.0	38.0	23.0	38.0
52-53	33.99749376710777	37.0	34.0	38.0	24.0	38.0
54-55	33.979435814285964	37.0	34.0	38.0	23.5	38.0
56-57	33.94125825967188	37.0	34.0	38.0	23.0	38.0
58-59	33.90954896525271	37.0	34.0	38.0	23.0	38.0
60-61	33.89961120700176	37.0	34.0	38.0	23.0	38.0
62-63	33.85957686071447	37.0	34.0	38.0	23.0	38.0
64-65	33.8650033997594	37.0	34.0	38.0	23.0	38.0
66-67	33.749699252052935	37.0	34.0	38.0	23.0	38.0
68-69	33.473813135275556	37.0	34.0	38.0	21.5	38.0
70-71	33.61172132433704	37.0	34.0	38.0	22.0	38.0
72-73	33.573626584375056	37.0	34.0	38.0	22.0	38.0
74-75	33.52501874226337	37.0	34.0	38.0	22.0	38.0
76	32.04992415921335	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	26.0
14	99.0
15	258.0
16	483.0
17	886.0
18	1023.0
19	1013.0
20	927.0
21	814.0
22	810.0
23	927.0
24	1149.0
25	1375.0
26	1605.0
27	2047.0
28	2482.0
29	3152.0
30	3772.0
31	4563.0
32	5766.0
33	7010.0
34	8990.0
35	12360.0
36	18190.0
37	34984.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.34942552783444	18.94189026622731	10.602018934044667	26.106665271893583
2	22.689471206653067	19.59133148525899	32.20879753125164	25.510399776836305
3	20.103038861865162	21.29295465243998	28.29733075300312	30.30667573269174
4	14.863050717436407	15.604023920358456	35.285143923148006	34.247781439057135
5	14.57625050124658	33.64628554492041	35.02362396917552	16.753839984657496
6	32.156493540457134	34.908555189427624	17.296058022560455	15.638893247554789
7	30.140174695329254	27.741164984221626	21.82384015900413	20.294820161444985
8	26.196453789424133	33.4353261153826	19.559949090782293	20.80827100441097
9	24.53144341579929	15.986714786338197	21.48735115155953	37.99449064630298
10-11	26.271422842896246	23.9334344543822	26.492407204002998	23.30273549871855
12-13	25.600624160956816	22.2091462245236	26.282755374235055	25.907474240284532
14-15	23.69283609672751	23.473159335390623	23.307965897798002	29.52603867008386
16-17	23.96786791498858	26.16986592743693	24.95597747441463	24.906288683159858
18-19	23.636173440033474	25.730948271353103	25.80940425754485	24.82347403106857
20-21	23.7442683543421	25.934933835451645	25.750126401311086	24.570671408895166
22-23	23.974841780427848	25.387921265059195	26.292344439214045	24.344892515298916
24-25	23.586920515368657	25.36482033579162	25.988981292605963	25.059277856233763
26-27	22.937479296336978	25.823787855013336	26.127151001621424	25.111581847028262
28-29	23.634429973673658	25.587112296668234	26.899070732430218	23.87938699722789
30-31	23.94563871890092	25.5570375019614	25.937113168401417	24.560210610736267
32-33	23.345886291124014	25.963265163798667	26.072667677877153	24.618180867200167
34-35	23.892462994926515	25.02004986313789	26.668497306344474	24.418989835591123
36-37	24.104730024234183	24.94115801035619	26.05741234722876	24.896699618180868
38-39	23.068675139913175	25.63375002179333	27.05075230573426	24.246822532559236
40-41	23.454417072022597	24.74458217828687	27.468312498910336	24.3326882507802
42-43	22.84115277995711	24.961643740084035	27.08562163293059	25.111581847028262
44-45	22.552609097407466	24.717994316299666	27.54328155238245	25.186115033910422
46-47	22.690342939832973	24.775964572763566	27.715448855414337	24.81824363198912
48-49	22.20304409226424	24.717994316299666	28.079833324616	24.999128266820094
50-51	21.83822375647262	24.731506180588244	27.988737207315584	25.44153285562355
52-53	21.57452446955036	24.725839914918843	28.569311505134507	25.13032411039629
54-55	21.044510696166117	25.010024931568946	28.424603797269732	25.520860574995208
56-57	21.068483358613594	25.22316369405652	28.265512491936466	25.442840455393412
58-59	21.043638962986208	25.121606778597204	28.166134909426923	25.66861934898966
60-61	21.04145963003644	24.788604703872238	27.96084174555852	26.209093920532805
62-63	20.808706871000922	24.46519169412626	28.408040866851476	26.31806056802134
64-65	21.51786181285632	24.576773541154523	27.627839670833552	26.277524975155604
66-67	21.42720156214586	24.517059818330804	27.102620429938806	26.95311818958453
68-69	21.178757605871994	24.49134368952351	26.926094461007377	27.40380424359712
70-71	21.88268214864794	24.219362937392123	26.85374060707499	27.04421430688495
72-73	21.934550272852483	23.739909688442562	26.589169586972822	27.736370451732135
74-75	22.25796328259846	23.93212685461234	26.42310441620029	27.38680544658891
76	22.81151385184023	23.527206792544938	25.947138099970363	27.71414125564447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	3.0
14	4.0
15	15.0
16	24.0
17	23.0
18	38.5
19	72.0
20	117.0
21	144.0
22	172.0
23	270.0
24	404.5
25	469.0
26	482.0
27	588.5
28	820.0
29	958.0
30	1088.5
31	1344.5
32	1730.0
33	1990.0
34	2214.5
35	2777.0
36	3375.0
37	3635.0
38	3930.0
39	4692.5
40	5516.5
41	6067.0
42	6261.0
43	6332.5
44	6522.5
45	6583.0
46	6525.0
47	6430.0
48	5903.5
49	5307.0
50	5142.0
51	4751.0
52	3990.0
53	3330.5
54	3041.0
55	2871.5
56	2412.5
57	1972.0
58	1821.0
59	1731.0
60	1589.5
61	1507.5
62	1477.0
63	1414.5
64	1395.5
65	1460.0
66	1491.5
67	1502.0
68	1482.0
69	1421.5
70	1364.0
71	1347.0
72	1331.0
73	1228.0
74	1093.0
75	1045.0
76	956.0
77	824.5
78	683.5
79	585.0
80	525.0
81	396.5
82	260.0
83	192.0
84	162.0
85	106.0
86	63.0
87	46.0
88	32.5
89	16.0
90	11.5
91	7.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	114714.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.78694841083041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5492243088767	43.97283679411406
2	14.37944710136475	17.194065240511183
3	5.163011781173451	9.260421570165803
4	2.260002332905634	5.404745715431421
5	1.2597690423422372	3.7658873372038286
6	0.7640265951242272	2.7407291176316755
7	0.5948909366616121	2.489669961818087
8	0.4140907500291613	1.9805777847516433
9	0.34118744896768927	1.8358700768868665
>10	1.2714335705120727	11.255818818975888
>50	0.002916132042458883	0.09937758250954548
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.717331799082936E-4	0.0
5	0.0	0.0	0.0	8.717331799082936E-4	0.0
6	0.0	0.0	0.0	8.717331799082936E-4	0.0
7	0.0	0.0	0.0	8.717331799082936E-4	0.0
8	0.0	0.0	0.0	8.717331799082936E-4	0.0
9	0.0	0.0	0.0	8.717331799082936E-4	0.0
10	0.0	0.0	0.0	8.717331799082936E-4	0.0
11	0.0	0.0	0.0	8.717331799082936E-4	0.0
12	0.0	0.0	0.0	0.0017434663598165873	0.0
13	0.0	0.0	0.0	0.0017434663598165873	0.0
14	0.0	0.0	0.0	0.0017434663598165873	0.0
15	0.0	0.0	0.0	0.002615199539724881	0.0
16	0.0	0.0	0.0	0.002615199539724881	0.0
17	0.0	0.0	0.0	0.0034869327196331745	0.0
18	0.0	0.0	0.0	0.0034869327196331745	0.0
19	0.0	0.0	0.0	0.0034869327196331745	0.0
20	0.0	0.0	0.0	0.0034869327196331745	0.0
21	0.0	0.0	0.0	0.0034869327196331745	0.0
22	0.0	0.0	0.0	0.0043586658995414685	0.0
23	0.0	0.0	0.0	0.0043586658995414685	0.0
24	0.0	0.0	0.0	0.007845598619174643	0.0
25	0.0	0.0	0.0	0.010460798158899524	0.0
26	0.0	0.0	0.0	0.012204264518716111	0.0
27	0.0	0.0	0.0	0.029638928116881985	0.0
28	0.0	0.0	0.0	0.04271492581550639	0.0
29	0.0	0.0	0.0	0.06276478895339714	0.0
30	0.0	0.0	0.0	0.07584078665202156	0.0
31	0.0	0.0	0.0	0.10896664748853671	0.0
32	0.0	0.0	0.0	0.14209250832505188	0.0
33	0.0	0.0	0.0	0.20311383091863242	0.0
34	0.0	0.0	0.0	0.2868002161898286	0.0
35	0.0	0.0	0.0	0.42017539271579757	0.0
36	0.0	0.0	0.0	0.6346217549732378	0.0
37	0.0	0.0	0.0	0.9493174329201318	0.0
38	0.0	0.0	0.0	1.3285213661802395	0.0
39	0.0	0.0	0.0	1.727775162578238	0.0
40	0.0	0.0	0.0	2.134874557595411	0.0
41	0.0	0.0	0.0	2.6343776696828636	0.0
42	0.0	0.0	0.0	3.1800826403054554	0.0
43	0.0	0.0	0.0	3.900134246909706	0.0
44	0.0	0.0	0.0	4.626287985773314	0.0
45	0.0	0.0	0.0	5.460536638945552	0.0
46	0.0	0.0	0.0	6.323552487054762	0.0
47	0.0	0.0	0.0	7.204002998762139	0.0
48	0.0	0.0	0.0	8.024303921055843	0.0
49	0.0	0.0	0.0	8.859424307407988	0.0
50	0.0	0.0	0.0	9.630908171626828	0.0
51	0.0	0.0	0.0	10.453824293460258	0.0
52	0.0	0.0	0.0	11.22966682357864	0.0
53	0.0	0.0	0.0	12.009868019596562	0.0
54	0.0	0.0	0.0	12.885088132224489	0.0
55	0.0	0.0	0.0	13.746360513973883	0.0
56	0.0	0.0	0.0	14.552713705389055	0.0
57	0.0	0.0	0.0	15.349477831825235	0.0
58	0.0	0.0	0.0	16.114859563784716	0.0
59	0.0	0.0	0.0	16.820963439510436	0.0
60	0.0	0.0	0.0	17.547117178374044	0.0
61	0.0	0.0	0.0	18.229684258242237	0.0
62	0.0	0.0	0.0	18.96368359572502	0.0
63	0.0	0.0	0.0	19.69506773366808	0.0
64	0.0	0.0	0.0	20.41076067437279	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCTA	15	0.0022197876	70.00001	1
CTCGCTT	15	0.0022197876	70.00001	45
CACGTGA	15	0.0022197876	70.00001	20
TGCTTAG	20	0.006936173	52.5	12
GTGTAAC	20	0.006936173	52.5	1
GCAATTT	20	0.006936173	52.5	54
CCCTATA	50	1.7009205E-4	35.0	2
CTCGAAT	40	0.0024103958	35.0	12
TATACTC	40	0.0024103958	35.0	5
AACAGGT	40	0.0024103958	35.0	34
AGAGCAA	40	0.0024103958	35.0	69
CAGGTAA	45	0.004292465	31.111113	4
ATAGCAC	45	0.004292465	31.111113	6
TGGCAAT	45	0.004292465	31.111113	27
CTAACCG	45	0.004292465	31.111113	21
TCGCTTC	45	0.004292465	31.111113	46
ACAATTA	45	0.004292465	31.111113	35
CGAATAA	45	0.004292465	31.111113	14
ACTCAGC	45	0.004292465	31.111113	8
TGTTATC	50	0.0071837637	27.999998	2
>>END_MODULE