FastQCFastQC Report
Wed 25 May 2016
SRR1781127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108629
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT27662.546281379742058No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG2880.2651225731618629No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC1940.1785895110881993No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCACCTCGTATGCCGT1310.12059394820904178No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTTA150.002219468670.01
TATGCGT150.002219468670.067
ATCGTAT301.01557816E-770.039
ATATCGT252.8236973E-670.037
CCGTCTT3850.058.18182447
TCGTATG4400.058.06818440
TCTCGTA3700.057.702738
CGTATGC4400.057.2727341
TATGCCG4400.057.2727343
CTCGTAT4100.057.1951239
GCCGTCT4100.057.1951246
ATCTCGT3900.055.64102637
ATGCCGT4350.055.5172444
CTTGAAA4100.055.48780457
CATCTCG3850.055.4545536
GTATGCC4550.055.38461742
TGCCGTC4400.054.88636845
CGTCTTC4100.054.63414848
TCATCTC3900.053.84615335
TCTTCTG4250.052.70588350
Produced by FastQC (version 0.11.5)