##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781127_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108629
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22820793710704	34.0	34.0	34.0	32.0	34.0
2	33.25374439606367	34.0	34.0	34.0	32.0	34.0
3	33.307579007447366	34.0	34.0	34.0	33.0	34.0
4	33.37277338463946	34.0	34.0	34.0	33.0	34.0
5	33.380009021532004	34.0	34.0	34.0	33.0	34.0
6	36.78815969952775	38.0	37.0	38.0	34.0	38.0
7	36.67623746881588	38.0	37.0	38.0	34.0	38.0
8	36.463725156265824	38.0	37.0	38.0	34.0	38.0
9	36.45309263640464	38.0	37.0	38.0	34.0	38.0
10-11	36.34446602656749	38.0	37.0	38.0	34.0	38.0
12-13	36.304527336162536	38.0	37.0	38.0	34.0	38.0
14-15	36.295915455357225	38.0	37.0	38.0	34.0	38.0
16-17	36.2353008865036	38.0	37.0	38.0	34.0	38.0
18-19	36.20673116755194	38.0	37.0	38.0	33.5	38.0
20-21	36.16011838459343	38.0	37.0	38.0	33.0	38.0
22-23	36.22635299965939	38.0	37.0	38.0	34.0	38.0
24-25	36.21356175606882	38.0	37.0	38.0	34.0	38.0
26-27	35.97406769831261	38.0	36.5	38.0	32.0	38.0
28-29	35.974247208388185	38.0	36.5	38.0	32.0	38.0
30-31	35.99300831269734	38.0	37.0	38.0	32.0	38.0
32-33	35.850974417512816	38.0	36.0	38.0	31.0	38.0
34-35	35.87498273941581	38.0	36.0	38.0	31.5	38.0
36-37	35.87741763249225	38.0	36.0	38.0	31.5	38.0
38-39	35.6915924845115	38.0	36.0	38.0	31.0	38.0
40-41	35.81980870669895	38.0	36.0	38.0	31.0	38.0
42-43	35.87656611033886	38.0	36.0	38.0	31.5	38.0
44-45	35.84343039151608	38.0	36.0	38.0	31.0	38.0
46-47	35.853782139207766	38.0	36.0	38.0	31.0	38.0
48-49	35.838095720295684	38.0	36.0	38.0	31.0	38.0
50-51	35.69822975448545	38.0	36.0	38.0	31.0	38.0
52-53	35.72796398751714	38.0	36.0	38.0	31.0	38.0
54-55	35.762236603485256	38.0	36.0	38.0	31.0	38.0
56-57	35.706814018356056	38.0	36.0	38.0	31.0	38.0
58-59	35.690957295013305	38.0	36.0	38.0	31.0	38.0
60-61	35.66699500133482	38.0	36.0	38.0	31.0	38.0
62-63	35.63878430253432	38.0	36.0	38.0	31.0	38.0
64-65	35.59004961842602	38.0	36.0	38.0	31.0	38.0
66-67	35.538985906157656	38.0	36.0	38.0	31.0	38.0
68-69	35.41239908311776	38.0	36.0	38.0	31.0	38.0
70-71	35.17708438814681	38.0	36.0	38.0	28.0	38.0
72-73	34.994398365077465	38.0	36.0	38.0	27.0	38.0
74-75	34.97829769214482	38.0	36.0	38.0	27.0	38.0
76	34.020316858297505	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	2.0
22	10.0
23	41.0
24	99.0
25	194.0
26	357.0
27	597.0
28	1075.0
29	1563.0
30	2387.0
31	3546.0
32	5075.0
33	6886.0
34	9469.0
35	12958.0
36	19031.0
37	45338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.793627852599215	18.86236640307837	10.372920674957884	28.971085069364534
2	21.274245367259205	19.144059137062847	34.56167321801728	25.020022277660665
3	20.02964217658268	21.479531248561617	29.638494324719915	28.85233225013578
4	14.4243249960876	18.518075283764006	34.81943127525799	32.238168444890405
5	14.414198786696003	33.77735227241344	36.38899373095582	15.419455209934732
6	31.048799123622604	38.20526747001261	16.128289867346655	14.617643539018127
7	29.030001196733835	27.61785526885086	20.93179537692513	22.42034815749017
8	25.62575371217631	36.3926759889164	18.12775593994237	19.853814358964918
9	23.892330777232598	15.312669729077871	20.102366771304165	40.69263272238537
10-11	25.790995038157398	25.205055740179876	26.532509734969484	22.47143948669324
12-13	25.087223485441275	21.89746752708761	26.642517191541852	26.372791795929263
14-15	22.767861252520046	22.6086035957249	22.71723020556205	31.906304946193004
16-17	25.430133758020418	27.197157296854428	23.418700347052813	23.95400859807234
18-19	24.952821069880052	25.455449281499416	24.445590035809957	25.146139612810575
20-21	23.461966878089644	24.974454335398466	24.282189838809156	27.281388947702734
22-23	23.551261633633743	26.61443997459242	24.65363761058281	25.180660781191026
24-25	25.146139612810575	26.300987765697926	24.552835798911893	24.000036822579606
26-27	24.655939021808173	27.085768993546843	24.894825507000892	23.36346647764409
28-29	23.192241482477055	25.17789908772059	27.0645960102735	24.565263419528854
30-31	23.382798331937142	26.530668605989195	25.020482559905734	25.06605050216793
32-33	22.706643713925377	27.16079499949369	24.640749707720776	25.49181157886016
34-35	23.362545913153944	24.85984405637537	26.644818602767216	25.132791427703467
36-37	23.424684016238757	26.096162166640585	24.86536744331624	25.613786373804416
38-39	22.590652588167064	25.40113597658084	27.001537342698544	25.006674092553556
40-41	22.936324554216643	24.250890646144217	27.62153752681144	25.1912472728277
42-43	24.506807574404625	24.01982895911773	27.28276979443795	24.190593672039697
44-45	22.20815804251167	25.90974785738615	27.949258485303186	23.932835614798993
46-47	24.05388984525311	23.89417190621289	26.496607719853817	25.555330528680187
48-49	23.40074933949498	23.6598882434709	26.941240368594023	25.998122048440102
50-51	21.663644146590688	23.87345920518462	28.51448508225244	25.948411565972258
52-53	21.06389638126099	23.97840355706119	30.62303804693038	24.334662014747444
54-55	20.263005274834526	24.32545636984599	29.185576595568403	26.225961759751083
56-57	21.89746752708761	24.204862421636946	27.319592374043765	26.578077677231676
58-59	20.52076333207523	24.120170488543575	29.884285043588726	25.47478113579247
60-61	21.791602610720894	25.81677084388147	26.868515773872538	25.523110771525097
62-63	19.563376262324056	27.821300021172984	26.4800375590312	26.13528615747176
64-65	20.04667261965037	28.206095978053742	25.699398871387935	26.047832530907954
66-67	20.250117371972493	28.07859779616861	25.074335582579238	26.596949249279657
68-69	19.826197424260556	28.481344760607204	24.891603531285384	26.800854283846853
70-71	20.27543289545149	27.620156680076224	24.89942832945162	27.204982095020668
72-73	20.228023824209004	26.155538576254962	25.104714210754036	28.511723388782002
74-75	20.345856078947612	26.087416803984205	24.915998490274234	28.65072862679395
76	20.639055869058907	26.343794014489685	24.61957672444743	28.397573392003977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	5.5
15	17.0
16	28.0
17	31.0
18	49.0
19	68.0
20	105.5
21	142.0
22	172.5
23	263.5
24	374.0
25	424.0
26	485.0
27	590.0
28	778.5
29	923.0
30	1015.0
31	1234.5
32	1629.0
33	1896.0
34	2060.0
35	2411.5
36	2875.0
37	3151.0
38	3483.5
39	4247.0
40	5195.0
41	6160.5
42	6609.0
43	6835.0
44	7065.0
45	6671.5
46	6274.0
47	6125.0
48	5550.5
49	4890.0
50	4655.0
51	4335.0
52	3605.0
53	2944.5
54	2694.0
55	2521.0
56	2145.0
57	1811.0
58	1680.0
59	1527.0
60	1351.0
61	1333.0
62	1338.0
63	1299.0
64	1284.0
65	1332.5
66	1367.0
67	1377.0
68	1342.5
69	1294.5
70	1235.5
71	1190.0
72	1214.5
73	1206.0
74	1109.5
75	1046.0
76	950.5
77	799.5
78	676.0
79	608.0
80	527.0
81	373.5
82	261.5
83	222.0
84	174.0
85	102.5
86	62.0
87	45.0
88	31.5
89	13.5
90	8.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	108629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.18798847453259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.02652639151155	33.28577083467582
2	19.06663069867818	19.519649449042152
3	6.970596169409225	10.704323891410212
4	2.8594550849743725	5.854790157324471
5	1.5987770883913317	4.0919091586961125
6	1.084434852980847	3.330602325345902
7	0.7391421634745077	2.6484640381481928
8	0.5431166262026796	2.2240838081911827
9	0.3542846866289003	1.6321608410277182
>10	1.7444474417768185	13.388689944674073
>50	0.005395198273536552	0.20896813926299607
>100	0.005395198273536552	0.5643060324591039
>500	0.0	0.0
>1k	0.001798399424512184	2.546281379742058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT	2766	2.546281379742058	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG	288	0.2651225731618629	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC	194	0.1785895110881993	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCACCTCGTATGCCGT	131	0.12059394820904178	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.205644901453572E-4	0.0
5	0.0	0.0	0.0	9.205644901453572E-4	0.0
6	0.0	0.0	0.0	9.205644901453572E-4	0.0
7	0.0	0.0	0.0	9.205644901453572E-4	0.0
8	0.0	0.0	0.0	9.205644901453572E-4	0.0
9	0.0	0.0	0.0	9.205644901453572E-4	0.0
10	0.0	0.0	0.0	9.205644901453572E-4	0.0
11	0.0	0.0	0.0	9.205644901453572E-4	0.0
12	0.0	0.0	0.0	0.0018411289802907143	0.0
13	0.0	0.0	0.0	0.0018411289802907143	0.0
14	0.0	0.0	0.0	0.0018411289802907143	0.0
15	0.0	0.0	0.0	0.0018411289802907143	0.0
16	0.0	0.0	0.0	0.0018411289802907143	0.0
17	0.0	0.0	0.0	0.0027616934704360716	0.0
18	0.0	0.0	0.0	0.0027616934704360716	0.0
19	0.0	0.0	0.0	0.0027616934704360716	0.0
20	0.0	0.0	0.0	0.0036822579605814286	0.0
21	0.0	0.0	0.0	0.0036822579605814286	0.0
22	0.0	0.0	0.0	0.004602822450726786	0.0
23	0.0	0.0	0.0	0.005523386940872143	0.0
24	0.0	0.0	0.0	0.0064439514310175	0.0
25	0.0	0.0	0.0	0.007364515921162857	0.0
26	0.0	0.0	0.0	0.009205644901453572	0.0
27	0.0	0.0	0.0	0.026696370214215358	0.0
28	0.0	0.0	0.0	0.04142540205654107	0.0
29	0.0	0.0	0.0	0.06351894982002965	0.0
30	0.0	9.205644901453572E-4	0.0	0.07732741717221	0.0
31	0.0	9.205644901453572E-4	0.0	0.11414999677802429	0.0
32	0.0	9.205644901453572E-4	0.0	0.14636975393311177	0.0
33	0.0	9.205644901453572E-4	0.0	0.20252418783197856	0.0
34	0.0	9.205644901453572E-4	0.0	0.2945806368465143	0.0
35	0.0	9.205644901453572E-4	0.0	0.42806248791759105	0.0
36	0.0	9.205644901453572E-4	0.0	0.6563624814736396	0.0
37	0.0	9.205644901453572E-4	0.0	0.9951302138471311	0.0
38	0.0	9.205644901453572E-4	0.0	1.3974168960406521	0.0
39	0.0	9.205644901453572E-4	0.0	1.811670916606063	0.0
40	0.0	9.205644901453572E-4	0.0	2.2388128400335083	0.0
41	0.0	9.205644901453572E-4	0.0	2.7469644385937455	0.0
42	0.0	9.205644901453572E-4	0.0	3.2956208747203783	0.0
43	0.0	9.205644901453572E-4	0.0	4.024707950915501	0.0
44	0.0	9.205644901453572E-4	0.0	4.791538171206584	0.0
45	0.0	9.205644901453572E-4	0.0	5.637536937650167	0.0
46	0.0	9.205644901453572E-4	0.0	6.53508731554189	0.0
47	0.0	9.205644901453572E-4	0.0	7.428034870982887	0.0
48	0.0	9.205644901453572E-4	0.0	8.252860654153126	0.0
49	0.0	9.205644901453572E-4	0.0	9.099779985086855	0.0
50	0.0	9.205644901453572E-4	0.0	9.893306575592153	0.0
51	0.0	9.205644901453572E-4	0.0	10.732861390604718	0.0
52	0.0	9.205644901453572E-4	0.0	11.51810290069871	0.0
53	0.0	9.205644901453572E-4	0.0	12.314391184674442	0.0
54	0.0	9.205644901453572E-4	0.0	13.192609708273112	0.0
55	0.0	9.205644901453572E-4	0.0	14.06162258697033	0.0
56	0.0	9.205644901453572E-4	0.0	14.873560467278535	0.0
57	0.0	9.205644901453572E-4	0.0	15.727844314133426	0.0
58	0.0	9.205644901453572E-4	0.0	16.517688646678142	0.0
59	0.0	9.205644901453572E-4	0.0	17.24401402940283	0.0
60	0.0	9.205644901453572E-4	0.0	17.968498283147227	0.0
61	0.0	9.205644901453572E-4	0.0	18.668127295657698	0.0
62	0.0	9.205644901453572E-4	0.0	19.39537324287253	0.0
63	0.0	9.205644901453572E-4	0.0	20.147474431321285	0.0
64	0.0	9.205644901453572E-4	0.0	20.8885288458883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTTA	15	0.0022194686	70.0	1
TATGCGT	15	0.0022194686	70.0	67
ATCGTAT	30	1.01557816E-7	70.0	39
ATATCGT	25	2.8236973E-6	70.0	37
CCGTCTT	385	0.0	58.181824	47
TCGTATG	440	0.0	58.068184	40
TCTCGTA	370	0.0	57.7027	38
CGTATGC	440	0.0	57.27273	41
TATGCCG	440	0.0	57.27273	43
CTCGTAT	410	0.0	57.19512	39
GCCGTCT	410	0.0	57.19512	46
ATCTCGT	390	0.0	55.641026	37
ATGCCGT	435	0.0	55.51724	44
CTTGAAA	410	0.0	55.487804	57
CATCTCG	385	0.0	55.45455	36
GTATGCC	455	0.0	55.384617	42
TGCCGTC	440	0.0	54.886368	45
CGTCTTC	410	0.0	54.634148	48
TCATCTC	390	0.0	53.846153	35
TCTTCTG	425	0.0	52.705883	50
>>END_MODULE