FastQCFastQC Report
Wed 25 May 2016
SRR1781126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618437
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG200.00695052652.565
CTAGCCG500.00720783527.99999842
TCGTTGA851.308065E-424.70588341
GATTTCG800.002665767221.87528
GGTTTAG2402.3646862E-1120.4166681
GTGCTAA900.00527416319.4444431
AGTGCGA950.00720693118.42105122
GGTTCTA1351.4111058E-418.1481481
ATCCGTG2357.912604E-917.8723470
GGTGTAT2551.1241355E-917.8431381
TCTATAC2751.6007107E-1017.818183
GTGGCGA1000.00968546117.534
GGTCTAG2852.6557245E-1017.1929821
GTTTAGG1957.823888E-616.1538472
GTAGACC1300.002289189716.1538473
GTGTAAA2653.4853656E-815.8490551
CTATACT3855.456968E-1215.4545454
GACGATC6150.015.36585470
GATCTTA1605.157331E-415.31251
ATCGTTG1400.003751309415.040
Produced by FastQC (version 0.11.5)