##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781126_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	618437
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.028390927450978	34.0	32.0	34.0	21.0	34.0
2	31.260244778368694	34.0	32.0	34.0	23.0	34.0
3	31.32272163534847	34.0	33.0	34.0	23.0	34.0
4	31.411270671062695	34.0	33.0	34.0	23.0	34.0
5	31.480703774192037	34.0	33.0	34.0	23.0	34.0
6	34.33437520717551	37.0	34.0	38.0	25.0	38.0
7	34.28053948906679	37.0	34.0	38.0	24.0	38.0
8	34.05061307780744	37.0	34.0	38.0	24.0	38.0
9	34.349367841833526	37.0	34.0	38.0	25.0	38.0
10-11	34.217719832416236	37.0	34.0	38.0	24.0	38.0
12-13	34.18039428430058	37.0	34.0	38.0	24.0	38.0
14-15	34.05885401423265	37.0	34.0	38.0	24.0	38.0
16-17	34.019723916906656	37.0	34.0	38.0	23.5	38.0
18-19	34.07753336233117	37.0	34.0	38.0	24.0	38.0
20-21	34.002872564222386	37.0	34.0	38.0	23.5	38.0
22-23	34.082533871679736	37.0	34.0	38.0	24.0	38.0
24-25	34.06532678348805	37.0	34.0	38.0	24.0	38.0
26-27	33.99816311119807	37.0	34.0	38.0	23.5	38.0
28-29	34.09586425133037	37.0	34.0	38.0	24.0	38.0
30-31	34.16482762189196	37.0	34.0	38.0	24.0	38.0
32-33	34.12081101227773	37.0	34.0	38.0	24.0	38.0
34-35	34.14781052880083	37.0	34.0	38.0	24.0	38.0
36-37	34.00344174103425	37.0	34.0	38.0	23.5	38.0
38-39	34.01347429083319	37.0	34.0	38.0	24.0	38.0
40-41	33.99813966499417	37.0	34.0	38.0	24.0	38.0
42-43	34.08780926755676	37.0	34.0	38.0	24.0	38.0
44-45	34.0857726817768	37.0	34.0	38.0	24.0	38.0
46-47	34.0748920261886	37.0	34.0	38.0	24.0	38.0
48-49	34.01300617524501	37.0	34.0	38.0	24.0	38.0
50-51	33.883462664750006	37.0	34.0	38.0	23.5	38.0
52-53	33.964135392934125	37.0	34.0	38.0	24.0	38.0
54-55	33.92640964237263	37.0	34.0	38.0	24.0	38.0
56-57	33.89365772099664	37.0	34.0	38.0	23.5	38.0
58-59	33.89341436556998	37.0	34.0	38.0	23.5	38.0
60-61	33.87199827953373	37.0	34.0	38.0	23.5	38.0
62-63	33.84372620008182	37.0	34.0	38.0	23.0	38.0
64-65	33.846974712056365	37.0	34.0	38.0	23.0	38.0
66-67	33.751379687825924	37.0	34.0	38.0	23.0	38.0
68-69	33.53881802026722	37.0	34.0	38.0	22.5	38.0
70-71	33.68081874144011	37.0	34.0	38.0	23.0	38.0
72-73	33.65668693820066	37.0	34.0	38.0	23.0	38.0
74-75	33.67498629609807	37.0	34.0	38.0	23.0	38.0
76	32.461927407318775	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	29.0
14	117.0
15	252.0
16	658.0
17	1252.0
18	2164.0
19	3055.0
20	3859.0
21	4904.0
22	6018.0
23	7352.0
24	8948.0
25	10488.0
26	12374.0
27	14414.0
28	16958.0
29	19812.0
30	23115.0
31	27190.0
32	31785.0
33	37771.0
34	46842.0
35	60502.0
36	89786.0
37	188789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.384939775595576	18.33250597878199	9.372822130629311	24.90973211499312
2	22.86053389431745	21.72557592770161	31.549050267044176	23.864839910936762
3	19.811395501886206	22.157956267170302	30.742177457040892	27.288470773902596
4	14.186893733719035	17.071908698865045	36.543576791168704	32.19762077624721
5	14.30606512870349	32.84667637932401	35.68867968766423	17.15857880430828
6	29.570675751935926	36.65902913312108	19.33681199540131	14.433483119541684
7	27.771785970114983	30.83078793797913	22.142918357084067	19.254507734821814
8	24.930429453606433	35.03056899894411	21.050486953400267	18.988514594049192
9	24.44905463288904	17.326582982583513	22.2638684296056	35.96049395492184
10-11	24.269246503685903	25.18275911693511	28.252109754105913	22.295884625273068
12-13	24.776897242564726	23.53449098291338	27.85392853273656	23.83468324178534
14-15	22.74532409930195	24.666700084244635	25.21000522284404	27.377970593609373
16-17	22.428638648722504	28.51672846223625	25.79219872032236	23.262434168718883
18-19	21.740937233703676	27.859507112284682	27.702660093105685	22.69689556090596
20-21	22.591872737239203	27.706055750221932	27.2301786600737	22.471892852465167
22-23	22.390073685759422	27.28838992492364	27.46100249499949	22.86053389431745
24-25	22.371801816514857	27.371906920187506	27.22387243971496	23.032418823582677
26-27	21.746515813251794	27.404165662791847	27.69772830538923	23.15159021856713
28-29	21.788880678225915	27.50441839670007	28.147410326354986	22.559290598719027
30-31	22.33032629030928	27.774615684378524	27.427126772815985	22.46793125249621
32-33	21.82784988608379	27.830805724754505	27.67735436269175	22.663990026469953
34-35	22.003534717360054	27.063063820567013	28.089360759462966	22.844040702609966
36-37	22.245192315466248	27.83185676148096	27.342882136741498	22.580068786311298
38-39	22.00652612958151	27.838486377755533	27.89532320996318	22.259664282699774
40-41	21.98995208889507	27.24764203952868	28.18225623628599	22.580149635290255
42-43	21.83148809013691	27.192179639963328	28.01190743762097	22.964424832278794
44-45	21.803999437291107	27.120870840522155	28.058153053585087	23.01697666860165
46-47	21.85226627772918	27.388966054747694	28.064459273943832	22.6943083935793
48-49	21.410264909764454	27.42462045446828	28.41906289565469	22.746051740112573
50-51	21.665020042461883	27.13809167304026	28.28558123139463	22.91130705310323
52-53	21.43104309735672	26.984397764040637	28.61358553902823	22.97097359957441
54-55	21.31922895945747	27.220234235661838	28.423428740518435	23.037108064362254
56-57	21.26298036653871	27.041527470999334	28.42591125326227	23.26958090919969
58-59	21.016938738055526	26.706385235623177	28.880908164082047	23.39576786223925
60-61	21.21492153581217	26.997259210749714	28.42416745494676	23.363651798491354
62-63	20.991356414694824	27.050436140874833	28.47960700832989	23.47860043610045
64-65	21.107149498330465	26.67960254513409	28.42408660570634	23.789161350829108
66-67	21.067954650007398	26.7519033947815	28.26135993383293	23.918782021378174
68-69	21.28834985758395	26.723525288450112	28.023986372041016	23.964138481924923
70-71	21.700294615108483	26.474773379212657	27.935964064071513	23.88896794160735
72-73	21.357880260561142	26.324857361612263	28.045653985992047	24.271608391834544
74-75	21.536701512689287	26.158044095175725	27.89856654296733	24.406687849167657
76	21.634054883520875	26.25570591668998	27.368996356945008	24.741242842844137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	1.5
7	2.5
8	4.0
9	2.5
10	2.0
11	10.0
12	17.0
13	38.0
14	76.5
15	152.0
16	307.5
17	405.0
18	555.5
19	869.0
20	1424.5
21	1817.0
22	2170.5
23	3119.0
24	4189.0
25	4664.0
26	5404.0
27	6847.5
28	8591.0
29	9631.0
30	10463.0
31	12497.0
32	14949.0
33	16199.0
34	17267.0
35	19603.0
36	22334.0
37	23797.0
38	24833.0
39	26970.5
40	28917.5
41	30157.5
42	30552.0
43	30700.5
44	30740.0
45	30123.5
46	29616.0
47	29115.5
48	27692.5
49	25977.5
50	25185.0
51	24167.5
52	22222.5
53	20798.5
54	20302.0
55	19645.0
56	18390.0
57	16828.5
58	15865.0
59	14641.5
60	12232.5
61	9898.0
62	8749.0
63	7974.0
64	6465.0
65	5229.0
66	4381.0
67	4035.0
68	3611.5
69	2923.5
70	2433.5
71	2208.0
72	2067.5
73	1716.0
74	1382.0
75	1259.0
76	1163.0
77	990.0
78	788.5
79	664.0
80	628.5
81	500.0
82	371.5
83	336.0
84	302.0
85	208.5
86	125.5
87	102.0
88	89.0
89	56.0
90	28.0
91	19.0
92	18.0
93	12.5
94	8.5
95	10.0
96	10.0
97	5.5
98	2.5
99	9.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.850938737494684E-4
58-59	4.042448947912237E-4
60-61	3.2339591583297893E-4
62-63	2.425469368747342E-4
64-65	3.2339591583297893E-4
66-67	4.042448947912237E-4
68-69	4.042448947912237E-4
70-71	4.850938737494684E-4
72-73	4.042448947912237E-4
74-75	3.2339591583297893E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	618437.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.09593339199881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01575169491547	50.40171697375687
2	17.064825327031834	23.240904176313087
3	5.581356329877918	11.40203006629133
4	1.7146940538330921	4.670547683098987
5	0.5714834632981086	1.9457849925688404
6	0.24982939892389255	1.0207419665090693
7	0.15134270801291555	0.7214076074948557
8	0.10537155367604048	0.5740299440428065
9	0.07770332866175993	0.47621526235990164
>10	0.4612243051142421	5.277282560600062
>50	0.006417836654743643	0.2693387669642999
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.2339591583297893E-4	0.0
2	0.0	0.0	0.0	3.2339591583297893E-4	0.0
3	0.0	0.0	0.0	3.2339591583297893E-4	0.0
4	0.0	0.0	0.0	3.2339591583297893E-4	0.0
5	0.0	0.0	0.0	3.2339591583297893E-4	0.0
6	0.0	0.0	0.0	3.2339591583297893E-4	0.0
7	0.0	0.0	0.0	4.850938737494684E-4	0.0
8	0.0	0.0	0.0	4.850938737494684E-4	0.0
9	0.0	0.0	0.0	6.467918316659579E-4	0.0
10	0.0	0.0	0.0	6.467918316659579E-4	0.0
11	0.0	0.0	0.0	6.467918316659579E-4	0.0
12	0.0	4.850938737494684E-4	0.0	8.084897895824474E-4	0.0
13	0.0	4.850938737494684E-4	0.0	0.0012935836633319157	0.0
14	0.0	4.850938737494684E-4	0.0	0.0012935836633319157	0.0
15	0.0	4.850938737494684E-4	0.0	0.0012935836633319157	0.0
16	0.0	4.850938737494684E-4	0.0	0.0016169795791648948	0.0
17	0.0	4.850938737494684E-4	0.0	0.0016169795791648948	0.0
18	0.0	4.850938737494684E-4	0.0	0.0017786775370813841	0.0
19	0.0	4.850938737494684E-4	0.0	0.0019403754949978737	0.0
20	0.0	4.850938737494684E-4	0.0	0.002102073452914363	0.0
21	0.0	4.850938737494684E-4	0.0	0.002748865284580321	0.0
22	0.0	4.850938737494684E-4	0.0	0.0030722612004133	0.0
23	0.0	4.850938737494684E-4	0.0	0.003395657116246279	0.0
24	0.0	4.850938737494684E-4	0.0	0.004204146905828726	0.0
25	0.0	4.850938737494684E-4	0.0	0.004365844863745216	0.0
26	0.0	4.850938737494684E-4	0.0	0.004850938737494684	0.0
27	0.0	4.850938737494684E-4	0.0	0.0063062203587430895	0.0
28	0.0	4.850938737494684E-4	0.0	0.013097534591235646	0.0
29	0.0	4.850938737494684E-4	0.0	0.020859036571227143	0.0
30	0.0	4.850938737494684E-4	0.0	0.028620538551218638	0.0
31	0.0	4.850938737494684E-4	0.0	0.03783732215245854	0.0
32	0.0	4.850938737494684E-4	0.0	0.04931787716452929	0.0
33	0.0	4.850938737494684E-4	0.0	0.06370899541909686	0.0
34	0.0	4.850938737494684E-4	0.0	0.08828708502240325	0.0
35	0.0	4.850938737494684E-4	0.0	0.12418403167986392	0.0
36	0.0	4.850938737494684E-4	0.0	0.17932303532938682	0.0
37	0.0	4.850938737494684E-4	0.0	0.2797374671955268	0.0
38	0.0	4.850938737494684E-4	0.0	0.41831261712995826	0.0
39	0.0	4.850938737494684E-4	0.0	0.5741894485614542	0.0
40	0.0	4.850938737494684E-4	0.0	0.7643462470712458	0.0
41	0.0	4.850938737494684E-4	0.0	0.9608092659397804	0.0
42	0.0	4.850938737494684E-4	0.0	1.1792632070849578	0.0
43	0.0	4.850938737494684E-4	0.0	1.406287139999709	0.0
44	0.0	4.850938737494684E-4	0.0	1.6797183868364927	0.0
45	0.0	4.850938737494684E-4	0.0	1.9846807354669918	0.0
46	0.0	4.850938737494684E-4	0.0	2.318425320606626	0.0
47	0.0	4.850938737494684E-4	0.0	2.660254803642085	0.0
48	0.0	4.850938737494684E-4	0.0	3.0231050212066872	0.0
49	0.0	4.850938737494684E-4	0.0	3.387087124476705	0.0
50	0.0	4.850938737494684E-4	0.0	3.75026073795714	0.0
51	0.0	4.850938737494684E-4	0.0	4.1140811432692415	0.0
52	0.0	4.850938737494684E-4	0.0	4.4428130917134645	0.0
53	0.0	4.850938737494684E-4	0.0	4.78755313799142	0.0
54	0.0	4.850938737494684E-4	0.0	5.126633755742299	0.0
55	0.0	4.850938737494684E-4	0.0	5.473960969346918	0.0
56	0.0	4.850938737494684E-4	0.0	5.846189668470677	0.0
57	0.0	4.850938737494684E-4	0.0	6.2061293227927825	0.0
58	0.0	4.850938737494684E-4	0.0	6.5870897116440315	0.0
59	0.0	4.850938737494684E-4	0.0	6.944442198639473	0.0
60	0.0	4.850938737494684E-4	0.0	7.335266162923628	1.6169795791648946E-4
61	0.0	4.850938737494684E-4	0.0	7.682431678570332	1.6169795791648946E-4
62	0.0	4.850938737494684E-4	0.0	8.038328883944525	1.6169795791648946E-4
63	0.0	4.850938737494684E-4	0.0	8.375307428242488	1.6169795791648946E-4
64	0.0	4.850938737494684E-4	0.0	8.714064650077534	1.6169795791648946E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGCGG	20	0.006950526	52.5	65
CTAGCCG	50	0.007207835	27.999998	42
TCGTTGA	85	1.308065E-4	24.705883	41
GATTTCG	80	0.0026657672	21.875	28
GGTTTAG	240	2.3646862E-11	20.416668	1
GTGCTAA	90	0.005274163	19.444443	1
AGTGCGA	95	0.007206931	18.421051	22
GGTTCTA	135	1.4111058E-4	18.148148	1
ATCCGTG	235	7.912604E-9	17.87234	70
GGTGTAT	255	1.1241355E-9	17.843138	1
TCTATAC	275	1.6007107E-10	17.81818	3
GTGGCGA	100	0.009685461	17.5	34
GGTCTAG	285	2.6557245E-10	17.192982	1
GTTTAGG	195	7.823888E-6	16.153847	2
GTAGACC	130	0.0022891897	16.153847	3
GTGTAAA	265	3.4853656E-8	15.849055	1
CTATACT	385	5.456968E-12	15.454545	4
GACGATC	615	0.0	15.365854	70
GATCTTA	160	5.157331E-4	15.3125	1
ATCGTTG	140	0.0037513094	15.0	40
>>END_MODULE