FastQCFastQC Report
Wed 25 May 2016
SRR1781126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554459
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT19360.34916919014751313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT3200.053.5937546
CGAGCAA200.006950149352.537
TATGCCG3250.051.6923143
CGTATGC3350.050.14925441
TCGTATG3500.048.040
CTCGTAT3300.047.72727239
CCGTCTT3650.046.986347
ATGCCGT3650.046.986344
TCTCGTA2850.046.66666438
CGTCTTC3650.046.02739748
TGCCGTC3850.045.45454445
ATCTCGT3000.044.33333637
GTATGCC3950.042.53164742
TCGCAGA3900.042.1794931
CGCAGAT3350.040.7462732
CTCGCAG4250.040.3529430
AGTACCG654.8640504E-737.6923075
CCTCGCA4600.036.52173629
TACCGCA751.4899906E-632.6666687
CAGATCT4350.031.3793134
Produced by FastQC (version 0.11.5)