##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781126_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554459
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.33853179405511	34.0	34.0	34.0	33.0	34.0
2	33.35392157039565	34.0	34.0	34.0	33.0	34.0
3	33.40204595831252	34.0	34.0	34.0	34.0	34.0
4	33.45812224168063	34.0	34.0	34.0	34.0	34.0
5	33.44395347536968	34.0	34.0	34.0	34.0	34.0
6	36.90321737044579	38.0	37.0	38.0	35.0	38.0
7	36.825047478713486	38.0	37.0	38.0	34.0	38.0
8	36.62312813030359	38.0	37.0	38.0	34.0	38.0
9	36.58454096696059	38.0	37.0	38.0	34.0	38.0
10-11	36.50642878914401	38.0	37.0	38.0	34.0	38.0
12-13	36.45473876337114	38.0	37.0	38.0	34.0	38.0
14-15	36.46451946852697	38.0	37.0	38.0	34.0	38.0
16-17	36.41679006022086	38.0	37.0	38.0	34.0	38.0
18-19	36.426414757448256	38.0	37.0	38.0	34.0	38.0
20-21	36.37220064964227	38.0	37.0	38.0	34.0	38.0
22-23	36.40406594536296	38.0	37.0	38.0	34.0	38.0
24-25	36.4060994591124	38.0	37.0	38.0	34.0	38.0
26-27	36.219248853386816	38.0	37.0	38.0	34.0	38.0
28-29	36.200109476083895	38.0	37.0	38.0	34.0	38.0
30-31	36.23570453360844	38.0	37.0	38.0	34.0	38.0
32-33	36.18250853534707	38.0	37.0	38.0	34.0	38.0
34-35	36.19145779940446	38.0	37.0	38.0	34.0	38.0
36-37	36.156039490746835	38.0	37.0	38.0	34.0	38.0
38-39	36.15773393524138	38.0	37.0	38.0	34.0	38.0
40-41	36.1415289498412	38.0	37.0	38.0	34.0	38.0
42-43	36.13105297235684	38.0	37.0	38.0	34.0	38.0
44-45	36.104528017400746	38.0	37.0	38.0	33.5	38.0
46-47	36.114154518188	38.0	37.0	38.0	33.5	38.0
48-49	36.099460014175975	38.0	37.0	38.0	33.5	38.0
50-51	36.02195653781433	38.0	37.0	38.0	32.0	38.0
52-53	36.049890974806075	38.0	37.0	38.0	32.5	38.0
54-55	36.05726482932012	38.0	37.0	38.0	32.5	38.0
56-57	35.99924791553568	38.0	37.0	38.0	32.0	38.0
58-59	36.00840008007806	38.0	37.0	38.0	32.0	38.0
60-61	36.00881940774701	38.0	37.0	38.0	32.0	38.0
62-63	35.98020142156589	38.0	37.0	38.0	32.0	38.0
64-65	35.93990087634974	38.0	37.0	38.0	32.0	38.0
66-67	35.9274905809086	38.0	37.0	38.0	32.0	38.0
68-69	35.89003064248213	38.0	37.0	38.0	32.0	38.0
70-71	35.870309617122274	38.0	37.0	38.0	31.5	38.0
72-73	35.8421650654061	38.0	37.0	38.0	31.5	38.0
74-75	35.84320481766912	38.0	37.0	38.0	31.5	38.0
76	35.07983998816865	38.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	1.0
20	4.0
21	19.0
22	56.0
23	135.0
24	310.0
25	644.0
26	1217.0
27	2169.0
28	3699.0
29	6124.0
30	9815.0
31	14725.0
32	21555.0
33	30385.0
34	42181.0
35	59613.0
36	94592.0
37	267214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.863699570211686	18.55448283822609	9.688362890673611	24.893454700888615
2	23.028213086991105	22.319053347497288	31.023574331014558	23.62915923449705
3	20.27868607056608	22.73387211678411	30.341648345504357	26.645793467145452
4	14.347679449697814	17.669115299778703	36.56753700453956	31.415668245983923
5	14.368961456122092	33.22229416422134	35.63653940147062	16.772204978185943
6	30.054701970749864	37.21393286068041	18.702915815236114	14.028449353333611
7	27.636308545807715	31.132148634975714	21.898463186637784	19.333079632578784
8	24.699752371230336	35.98534787964484	20.575552024586127	18.739347724538693
9	24.38773651433199	17.368461870039084	21.930386196274206	36.31341541935472
10-11	24.079147421179925	25.651581090756935	28.13400089095857	22.135270597104565
12-13	24.813917710777535	23.73196214688552	27.707188448559766	23.746931693777178
14-15	22.557664317830532	24.713459426215465	25.10744707904462	27.621429176909384
16-17	22.63539774807515	28.737652378264215	25.64977753089047	22.97717234277016
18-19	21.995314351466924	28.01830252552488	27.472635487926066	22.513747635082126
20-21	22.467937214473928	27.72747849705749	27.068277365864745	22.736306922603834
22-23	22.18522920540563	27.5675027369021	27.41086356249966	22.83640449519261
24-25	22.731617666951028	27.569667008741856	27.00289832070541	22.69581700360171
26-27	21.954103008518214	27.766705924153097	27.493286248397087	22.785904818931606
28-29	21.70908940065902	27.451443659495112	28.04625770345508	22.793209236390787
30-31	22.278383072508518	27.66471461370453	27.5508198081373	22.50608250564965
32-33	21.990534917820796	27.791414694323656	27.57354466245475	22.644505725400794
34-35	21.943191471326102	27.342418465567338	27.974836732743086	22.73955333036347
36-37	22.41175632463356	27.920098690795893	27.318611475330005	22.349533509240537
38-39	21.799808461942185	27.755614030974336	28.08314050272428	22.361437004359207
40-41	21.818024416593474	27.21157019725534	28.336540663962527	22.633864722188658
42-43	22.20055946427058	27.14114118446991	28.15988197504234	22.498417376217176
44-45	21.78042019337769	27.177573093772487	28.33545852804265	22.706548184807176
46-47	21.870237474727617	27.18884534293789	28.170252444274507	22.770664738059985
48-49	21.45352496758101	27.144387592229542	28.646302071027797	22.75578536916165
50-51	21.54613776672396	27.047626605393727	28.581644449815048	22.82459117806727
52-53	21.227358560326373	26.95429238230419	29.046512005396252	22.771837051973183
54-55	21.140968042722726	27.02192587729661	28.782200306965887	23.054905773014777
56-57	21.253780712370077	26.91425335326868	28.538178657033253	23.29378727732799
58-59	20.790987250635304	26.551918176095977	29.36980011146	23.28729446180872
60-61	21.247648608824097	27.307880294124544	28.114973334367377	23.329497762683985
62-63	20.594579581177328	27.496171944183427	28.311290825832025	23.597957648807217
64-65	20.663926458042887	27.24511641077158	28.121375971893325	23.96958115929221
66-67	20.62731419275366	27.37749770497007	27.80936011499498	24.185827987281296
68-69	20.895774078876887	27.461092704780697	27.755974742947632	23.887158473394788
70-71	21.13627878706992	27.166841912567026	27.314282931650492	24.382596368712566
72-73	20.68448704052058	26.863122431054414	27.656418238318793	24.79597229010621
74-75	20.795856862274757	26.833634227237724	27.22969597391331	25.140812936574207
76	20.912817719614978	26.817853078406156	26.956186120163977	25.313143081814886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	2.0
11	6.0
12	9.0
13	15.5
14	49.5
15	143.0
16	299.0
17	389.0
18	525.0
19	943.5
20	1434.5
21	1643.0
22	2076.5
23	2846.0
24	3843.0
25	4504.0
26	5159.0
27	6433.0
28	7863.0
29	8674.0
30	9580.0
31	11426.0
32	13411.0
33	14456.0
34	15522.5
35	17592.0
36	20024.0
37	21453.0
38	22281.5
39	24183.5
40	25879.0
41	26629.5
42	26758.0
43	27156.5
44	27783.0
45	27590.5
46	27170.0
47	26635.5
48	25154.0
49	23336.0
50	22465.0
51	21402.0
52	19447.0
53	18391.0
54	18227.0
55	17691.5
56	16380.0
57	14680.5
58	13757.0
59	12702.0
60	10717.0
61	8787.0
62	7787.0
63	7077.0
64	5674.5
65	4591.5
66	3824.5
67	3448.0
68	3194.0
69	2606.0
70	2108.5
71	1945.0
72	1836.0
73	1538.0
74	1197.5
75	1046.0
76	978.0
77	847.5
78	714.5
79	644.0
80	618.0
81	500.0
82	364.0
83	320.0
84	271.0
85	202.0
86	134.0
87	86.0
88	70.0
89	43.0
90	22.5
91	10.5
92	8.0
93	5.5
94	2.5
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	554459.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.286531428083514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.66803210433933	40.245659133829896
2	22.36507272167293	27.41355464496363
3	7.4740841165280045	13.741820733112
4	2.322397038146133	5.693266362673242
5	0.7790840218987425	2.3873678696607494
6	0.32431634113287394	1.1925734180088559
7	0.19885438133915107	0.8530966704087791
8	0.138700067949863	0.6800356858789278
9	0.10975075970403372	0.6053619045471574
>10	0.6076225959498218	6.31576758300022
>50	0.01031719267627443	0.3918483192537397
>100	0.0014738822190448295	0.12989328741377068
>500	0.0	0.0
>1k	2.9477644380731063E-4	0.34975438724900926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	1936	0.34916919014751313	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.607119732928855E-4	0.0
2	0.0	0.0	0.0	3.607119732928855E-4	0.0
3	0.0	0.0	0.0	3.607119732928855E-4	0.0
4	0.0	0.0	0.0	3.607119732928855E-4	0.0
5	0.0	0.0	0.0	3.607119732928855E-4	0.0
6	0.0	0.0	0.0	3.607119732928855E-4	0.0
7	0.0	0.0	0.0	3.607119732928855E-4	0.0
8	0.0	0.0	0.0	3.607119732928855E-4	0.0
9	0.0	0.0	0.0	5.410679599393282E-4	0.0
10	0.0	0.0	0.0	5.410679599393282E-4	0.0
11	0.0	0.0	0.0	5.410679599393282E-4	0.0
12	0.0	0.0	0.0	5.410679599393282E-4	0.0
13	0.0	0.0	0.0	0.0010821359198786564	0.0
14	0.0	0.0	0.0	0.0010821359198786564	0.0
15	0.0	0.0	0.0	0.0010821359198786564	0.0
16	0.0	0.0	0.0	0.001442847893171542	0.0
17	0.0	0.0	0.0	0.001442847893171542	0.0
18	0.0	1.8035598664644276E-4	0.0	0.0019839158531108703	0.0
19	0.0	1.8035598664644276E-4	0.0	0.002164271839757313	0.0
20	0.0	1.8035598664644276E-4	0.0	0.0025249838130501985	0.0
21	0.0	1.8035598664644276E-4	0.0	0.002885695786343084	0.0
22	0.0	1.8035598664644276E-4	0.0	0.002885695786343084	0.0
23	0.0	1.8035598664644276E-4	0.0	0.0032464077596359693	0.0
24	0.0	1.8035598664644276E-4	0.0	0.004328543679514626	0.0
25	0.0	1.8035598664644276E-4	0.0	0.004689255652807512	0.0
26	0.0	1.8035598664644276E-4	0.0	0.0048696116394539544	0.0
27	0.0	1.8035598664644276E-4	0.0	0.006312459532625496	0.0
28	0.0	1.8035598664644276E-4	0.0	0.01316598702519032	0.0
29	0.0	1.8035598664644276E-4	0.0	0.022724854317451786	0.0
30	0.0	1.8035598664644276E-4	0.0	0.030660517729895267	0.0
31	0.0	1.8035598664644276E-4	0.0	0.04166223291532827	0.0
32	0.0	1.8035598664644276E-4	0.0	0.05464786395387215	0.0
33	0.0	1.8035598664644276E-4	0.0	0.07196203867193066	0.0
34	0.0	1.8035598664644276E-4	0.0	0.10027792857542217	0.0
35	0.0	1.8035598664644276E-4	0.0	0.14031695761093246	0.0
36	0.0	1.8035598664644276E-4	0.0	0.20308084096389453	0.0
37	0.0	1.8035598664644276E-4	0.0	0.3183283164309714	0.0
38	0.0	1.8035598664644276E-4	0.0	0.4737951769202051	0.0
39	0.0	1.8035598664644276E-4	0.0	0.6512654677803048	0.0
40	0.0	1.8035598664644276E-4	0.0	0.8642658880097537	0.0
41	0.0	1.8035598664644276E-4	0.0	1.0826769878385958	0.0
42	0.0	1.8035598664644276E-4	0.0	1.324714721918122	0.0
43	0.0	1.8035598664644276E-4	0.0	1.5777541711830811	0.0
44	0.0	1.8035598664644276E-4	0.0	1.8796700928292263	0.0
45	0.0	1.8035598664644276E-4	0.0	2.214771516018317	0.0
46	0.0	1.8035598664644276E-4	0.0	2.5787298970708386	0.0
47	0.0	1.8035598664644276E-4	1.8035598664644276E-4	2.9538703492954395	0.0
48	0.0	1.8035598664644276E-4	1.8035598664644276E-4	3.3445214163716344	0.0
49	0.0	1.8035598664644276E-4	1.8035598664644276E-4	3.7385992471941116	0.0
50	0.0	1.8035598664644276E-4	1.8035598664644276E-4	4.133398501963175	0.0
51	0.0	1.8035598664644276E-4	1.8035598664644276E-4	4.528017400745592	0.0
52	0.0	1.8035598664644276E-4	1.8035598664644276E-4	4.882597270492498	0.0
53	0.0	1.8035598664644276E-4	1.8035598664644276E-4	5.258459146663685	0.0
54	0.0	1.8035598664644276E-4	1.8035598664644276E-4	5.6216961037696205	0.0
55	0.0	1.8035598664644276E-4	1.8035598664644276E-4	6.00098474368709	0.0
56	0.0	1.8035598664644276E-4	1.8035598664644276E-4	6.399932186149021	0.0
57	0.0	1.8035598664644276E-4	1.8035598664644276E-4	6.804831376170285	0.0
58	0.0	1.8035598664644276E-4	1.8035598664644276E-4	7.2131573299378315	0.0
59	0.0	1.8035598664644276E-4	1.8035598664644276E-4	7.606874448787016	0.0
60	0.0	1.8035598664644276E-4	1.8035598664644276E-4	8.023496777940299	0.0
61	0.0	1.8035598664644276E-4	1.8035598664644276E-4	8.392865838592213	0.0
62	0.0	1.8035598664644276E-4	1.8035598664644276E-4	8.773056258442915	0.0
63	0.0	1.8035598664644276E-4	1.8035598664644276E-4	9.133587875749154	0.0
64	0.0	1.8035598664644276E-4	1.8035598664644276E-4	9.498628392721553	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	320	0.0	53.59375	46
CGAGCAA	20	0.0069501493	52.5	37
TATGCCG	325	0.0	51.69231	43
CGTATGC	335	0.0	50.149254	41
TCGTATG	350	0.0	48.0	40
CTCGTAT	330	0.0	47.727272	39
CCGTCTT	365	0.0	46.9863	47
ATGCCGT	365	0.0	46.9863	44
TCTCGTA	285	0.0	46.666664	38
CGTCTTC	365	0.0	46.027397	48
TGCCGTC	385	0.0	45.454544	45
ATCTCGT	300	0.0	44.333336	37
GTATGCC	395	0.0	42.531647	42
TCGCAGA	390	0.0	42.17949	31
CGCAGAT	335	0.0	40.74627	32
CTCGCAG	425	0.0	40.35294	30
AGTACCG	65	4.8640504E-7	37.692307	5
CCTCGCA	460	0.0	36.521736	29
TACCGCA	75	1.4899906E-6	32.666668	7
CAGATCT	435	0.0	31.37931	34
>>END_MODULE