FastQCFastQC Report
Wed 25 May 2016
SRR1781125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172866
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAG252.3853389E-455.99999611
CTCAACG407.44707E-752.50000446
CGCAAGG200.00694209752.50000412
CAACGGC451.677432E-646.66666448
GCACGAC305.865475E-446.66666411
AGCCTTA451.677432E-646.6666646
TCAACGG451.677432E-646.66666447
ACGGCTA503.4636942E-642.050
ACTCAAG503.4636942E-642.011
CCGGACG350.001252811640.045
CGGACGT350.001252811640.046
GGAATCT556.666254E-638.18181634
ATTACAT556.666254E-638.18181625
AACGGCT556.666254E-638.18181649
CGGCTAC556.666254E-638.18181651
ATTCCCG400.002413769735.00000441
GATATAG400.002413769735.0000042
CTCGCAA400.002413769735.00000426
GAATCTT601.2105878E-535.035
ATCCGCT501.7044898E-435.049