##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781125_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172866
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09378362431016	34.0	32.0	34.0	21.0	34.0
2	31.16676500873509	34.0	32.0	34.0	23.0	34.0
3	31.178959425219535	34.0	32.0	34.0	23.0	34.0
4	31.310309719667256	34.0	33.0	34.0	23.0	34.0
5	31.276005692270314	34.0	33.0	34.0	23.0	34.0
6	33.996789420707366	37.0	34.0	38.0	24.0	38.0
7	33.9964943945021	37.0	34.0	38.0	24.0	38.0
8	33.75612902479377	37.0	34.0	38.0	23.0	38.0
9	34.06493468929691	37.0	34.0	38.0	24.0	38.0
10-11	33.905499057073115	37.0	34.0	38.0	23.0	38.0
12-13	33.86070424490646	37.0	34.0	38.0	23.0	38.0
14-15	33.740402971087434	37.0	34.0	38.0	22.5	38.0
16-17	33.69343017134659	37.0	34.0	38.0	22.5	38.0
18-19	33.738994365577966	37.0	34.0	38.0	22.5	38.0
20-21	33.65896127636435	37.0	34.0	38.0	22.5	38.0
22-23	33.7462658938137	37.0	34.0	38.0	22.5	38.0
24-25	33.728740758737985	37.0	34.0	38.0	23.0	38.0
26-27	33.63378570684808	37.0	34.0	38.0	22.0	38.0
28-29	33.723956706350585	37.0	34.0	38.0	23.0	38.0
30-31	33.76570869922368	37.0	34.0	38.0	23.0	38.0
32-33	33.742537572454964	37.0	34.0	38.0	23.0	38.0
34-35	33.746025823470205	37.0	34.0	38.0	23.0	38.0
36-37	33.606351162171855	37.0	34.0	38.0	22.5	38.0
38-39	33.6079651290595	37.0	34.0	38.0	22.0	38.0
40-41	33.60635116217186	37.0	34.0	38.0	22.0	38.0
42-43	33.696626288570336	37.0	34.0	38.0	23.0	38.0
44-45	33.7269416773686	37.0	34.0	38.0	23.0	38.0
46-47	33.7109321671121	37.0	34.0	38.0	22.5	38.0
48-49	33.67295188180441	37.0	34.0	38.0	22.5	38.0
50-51	33.53790219013571	37.0	34.0	38.0	22.0	38.0
52-53	33.60904978422593	37.0	34.0	38.0	22.0	38.0
54-55	33.579798803697656	37.0	34.0	38.0	22.0	38.0
56-57	33.54715212939502	37.0	34.0	38.0	22.0	38.0
58-59	33.531052954311434	37.0	34.0	38.0	22.0	38.0
60-61	33.50580796686451	37.0	34.0	38.0	22.0	38.0
62-63	33.45846493816019	37.0	34.0	38.0	22.0	38.0
64-65	33.478839100806404	37.0	34.0	38.0	22.0	38.0
66-67	33.37767403653697	37.0	34.0	38.0	21.5	38.0
68-69	33.131561440653456	37.0	33.5	38.0	21.0	38.0
70-71	33.26713176680203	37.0	33.5	38.0	21.0	38.0
72-73	33.23022167459188	37.0	33.0	38.0	21.0	38.0
74-75	33.172034986637044	37.0	32.5	38.0	21.0	38.0
76	31.681412192102552	36.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	30.0
14	95.0
15	271.0
16	577.0
17	1048.0
18	1406.0
19	1639.0
20	1581.0
21	1618.0
22	1758.0
23	1991.0
24	2296.0
25	2792.0
26	3349.0
27	3844.0
28	4502.0
29	5427.0
30	6489.0
31	7357.0
32	8981.0
33	10606.0
34	13181.0
35	17123.0
36	25261.0
37	49640.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.228674233221106	18.384181967535547	9.974199669107865	26.41294413013548
2	23.772748834357248	19.612300857311443	30.56008700380642	26.054863304524893
3	20.053683199703816	20.823065264424468	28.36069556766513	30.76255596820659
4	14.569088195480893	15.886293429592863	35.50495759721402	34.039660777712214
5	14.39901426538475	32.917404232179834	35.007462427545036	17.67611907489038
6	31.00146934619879	35.07630187544109	18.06890886582671	15.853319912533406
7	30.3188596947925	27.824442053382388	21.65955132877489	20.197146923050223
8	26.74672868001805	32.795922853539736	19.2709960316083	21.186352434833918
9	24.999710758622285	16.247266668980597	20.93760484999942	37.81541772239769
10-11	26.0988279939375	23.69320745548575	26.455751854037228	23.752212696539516
12-13	25.99499033933799	22.334351462982887	25.905036270868763	25.76562192681036
14-15	23.850265523584742	23.400205939860932	23.304756285215138	29.444772251339185
16-17	24.076452280957504	26.028831580530586	24.776416415026667	25.118299723485244
18-19	23.65531683500515	25.753763030324066	25.827519581641273	24.763400553029513
20-21	23.779401386044682	26.067011442388903	25.655710203278844	24.497876968287574
22-23	24.043189522520333	25.20883227470989	26.151759166059257	24.596219036710515
24-25	23.557553249337637	25.531336410861595	26.075688683720337	24.835421656080435
26-27	22.894612011615934	25.794546064581812	26.2859671653188	25.02487475848345
28-29	23.411775594969512	25.885657098561893	26.647229646084252	24.055337660384343
30-31	23.57953559404394	25.598440410491364	26.20469033818102	24.617333657283677
32-33	23.226082630476785	25.915159719088777	25.86743489176588	24.991322758668563
34-35	23.870801661402474	25.34304027396943	26.270348130922216	24.515809933705874
36-37	23.928939178323095	25.23573172283734	26.070193097543765	24.7651360012958
38-39	23.281038492242544	25.622736686219383	26.755984404104915	24.340240417433158
40-41	23.515324008191314	24.723485242904907	27.26475998750477	24.496430761399
42-43	22.94667545960455	25.05871599967605	26.977254058056527	25.017354482662874
44-45	22.578471185773953	24.845834345678156	27.231786470445318	25.343907998102576
46-47	22.860770770423333	24.587252554001367	27.59912302014277	24.95285365543253
48-49	22.138535050270153	24.787986070135247	28.02835722467113	25.04512165492347
50-51	22.183078222438187	24.762532828896372	27.64308770955538	25.411301239110063
52-53	21.940983189291128	24.662455312207143	28.176448810060972	25.22011268844076
54-55	21.309569261740307	24.96037393125311	28.038191431513425	25.691865375493155
56-57	21.181146090035057	25.113961102819527	28.02112619022827	25.683766616917147
58-59	21.512327467518194	24.997686068978282	27.667962468038827	25.822023995464694
60-61	21.275728020547707	24.60229310564252	27.992780535212248	26.129198338597526
62-63	20.91273009151597	24.61559820901739	28.120335982784354	26.351335716682282
64-65	21.825286638205316	24.684148415535734	27.341119711221406	26.149445235037543
66-67	21.717978087073224	24.428748279013803	26.87428412759016	26.978989506322815
68-69	21.575671329237675	24.337058762278296	26.82771626577812	27.259553642705907
70-71	22.071141809262667	24.30987007277313	26.882093644788448	26.736894473175752
72-73	22.01069036132033	23.893362488864206	26.475420267721816	27.620526882093642
74-75	22.152129395022733	23.921997385257946	26.421332130089205	27.50454108963012
76	22.599585806347115	23.34640704360603	26.0311455115523	28.022861638494557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	6.5
16	16.5
17	21.0
18	41.0
19	46.5
20	48.5
21	65.0
22	100.5
23	184.5
24	324.5
25	416.0
26	529.5
27	653.0
28	803.5
29	944.0
30	1117.0
31	1473.0
32	2126.0
33	2596.0
34	2859.0
35	3541.0
36	4484.0
37	5008.0
38	5653.0
39	6990.0
40	8431.0
41	9502.5
42	9825.0
43	10139.5
44	10433.5
45	10551.5
46	10690.0
47	10549.0
48	9833.5
49	8959.0
50	8659.0
51	8008.5
52	6666.0
53	5581.5
54	5189.0
55	4845.0
56	4162.5
57	3422.5
58	3021.0
59	2792.0
60	2419.0
61	2223.5
62	2172.0
63	2092.5
64	2048.0
65	2032.0
66	1893.0
67	1805.0
68	1803.5
69	1816.5
70	1755.0
71	1679.0
72	1620.0
73	1478.0
74	1350.5
75	1306.0
76	1205.0
77	1000.0
78	808.5
79	721.0
80	665.0
81	531.0
82	393.5
83	334.0
84	279.5
85	201.5
86	143.0
87	108.0
88	91.0
89	53.0
90	29.5
91	20.5
92	14.0
93	13.0
94	7.5
95	5.0
96	7.0
97	4.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	172866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.54552080802472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51829044316243	39.365751506947575
2	12.747131652297918	13.651036062614974
3	5.29050798383786	8.49849016000833
4	2.3670620773103432	5.069822868580288
5	1.3342408331712798	3.57213101477445
6	0.9334284047449277	2.9988546041442503
7	0.6795445215099069	2.5470595721541542
8	0.4775177718718265	2.045515023197159
9	0.4148570687755235	1.9992364027628335
>10	2.214731747369331	19.505281547557068
>50	0.02160713899872518	0.6878159962051531
>100	0.001080356949936259	0.05900524105376419
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011569655108581212	0.0
2	0.0	0.0	0.0	0.0011569655108581212	0.0
3	0.0	0.0	0.0	0.0011569655108581212	0.0
4	0.0	0.0	0.0	0.0028924137771453033	0.0
5	0.0	0.0	0.0	0.004627862043432485	0.0
6	0.0	0.0	0.0	0.004627862043432485	0.0
7	0.0	0.0	0.0	0.004627862043432485	0.0
8	0.0	0.0	0.0	0.004627862043432485	0.0
9	0.0	0.0	0.0	0.005784827554290607	0.0
10	0.0	0.0	0.0	0.006363310309719667	0.0
11	0.0	0.0	0.0	0.006363310309719667	0.0
12	0.0	0.0	0.0	0.006363310309719667	0.0
13	0.0	0.0	0.0	0.006363310309719667	0.0
14	0.0	0.0	0.0	0.006363310309719667	0.0
15	0.0	0.0	0.0	0.006363310309719667	0.0
16	0.0	0.0	0.0	0.007520275820577789	0.0
17	0.0	0.0	0.0	0.009834206842294031	0.0
18	0.0	0.0	0.0	0.009834206842294031	0.0
19	0.0	0.0	0.0	0.011569655108581213	0.0
20	0.0	0.0	0.0	0.011569655108581213	0.0
21	0.0	0.0	0.0	0.011569655108581213	0.0
22	0.0	0.0	0.0	0.013883586130297457	0.0
23	0.0	0.0	0.0	0.014462068885726516	0.0
24	0.0	0.0	0.0	0.01735448266287182	0.0
25	0.0	0.0	0.0	0.017932965418300882	0.0
26	0.0	0.0	0.0	0.01851144817372994	0.0
27	0.0	0.0	0.0	0.027767172260594913	0.0
28	0.0	0.0	0.0	0.04049379288003425	0.0
29	0.0	0.0	0.0	0.059583723809193245	0.0
30	0.0	0.0	0.0	0.08503696504807191	0.0
31	0.0	0.0	0.0	0.10701930975437622	0.0
32	0.0	0.0	0.0	0.13131558548239677	0.0
33	0.0	0.0	0.0	0.17007393009614383	0.0
34	0.0	0.0	0.0	0.2504830331007833	0.0
35	0.0	0.0	0.0	0.35229599805629797	0.0
36	0.0	0.0	0.0	0.514849652331864	0.0
37	0.0	0.0	0.0	0.7763238577857994	0.0
38	0.0	0.0	0.0	1.0811842698969143	0.0
39	0.0	0.0	0.0	1.4346372334640705	0.0
40	0.0	0.0	0.0	1.8361042657318385	0.0
41	0.0	0.0	0.0	2.2971550218087997	0.0
42	0.0	0.0	0.0	2.8091122603635186	0.0
43	0.0	0.0	0.0	3.450649636134347	0.0
44	0.0	0.0	0.0	4.136730184073213	0.0
45	0.0	0.0	0.0	4.890493214397279	0.0
46	0.0	0.0	0.0	5.6714449342265105	0.0
47	0.0	0.0	0.0	6.41074589566485	0.0
48	0.0	0.0	0.0	7.086992236761422	0.0
49	0.0	0.0	0.0	7.80893871553689	0.0
50	0.0	0.0	0.0	8.441798849976282	0.0
51	0.0	0.0	0.0	9.119780639339142	0.0
52	0.0	0.0	0.0	9.801811807990005	0.0
53	0.0	0.0	0.0	10.478636631842004	0.0
54	0.0	0.0	0.0	11.278678282600396	0.0
55	0.0	0.0	0.0	12.079876898869644	0.0
56	0.0	0.0	0.0	12.802980343155971	0.0
57	0.0	0.0	0.0	13.506993856513137	0.0
58	0.0	0.0	0.0	14.190181990674859	0.0
59	0.0	0.0	0.0	14.821306676847962	0.0
60	0.0	0.0	0.0	15.440283225157058	0.0
61	0.0	0.0	0.0	16.09686115256904	0.0
62	0.0	0.0	0.0	16.702532597503268	0.0
63	0.0	0.0	0.0	17.34291300776324	0.0
64	0.0	0.0	0.0	18.026679624680387	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCAAG	25	2.3853389E-4	55.999996	11
CTCAACG	40	7.44707E-7	52.500004	46
CGCAAGG	20	0.006942097	52.500004	12
CAACGGC	45	1.677432E-6	46.666664	48
GCACGAC	30	5.865475E-4	46.666664	11
AGCCTTA	45	1.677432E-6	46.666664	6
TCAACGG	45	1.677432E-6	46.666664	47
ACGGCTA	50	3.4636942E-6	42.0	50
ACTCAAG	50	3.4636942E-6	42.0	11
CCGGACG	35	0.0012528116	40.0	45
CGGACGT	35	0.0012528116	40.0	46
GGAATCT	55	6.666254E-6	38.181816	34
ATTACAT	55	6.666254E-6	38.181816	25
AACGGCT	55	6.666254E-6	38.181816	49
CGGCTAC	55	6.666254E-6	38.181816	51
ATTCCCG	40	0.0024137697	35.000004	41
GATATAG	40	0.0024137697	35.000004	2
CTCGCAA	40	0.0024137697	35.000004	26
GAATCTT	60	1.2105878E-5	35.0	35
ATCCGCT	50	1.7044898E-4	35.0	49
>>END_MODULE