FastQCFastQC Report
Wed 25 May 2016
SRR1781125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156381
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT32412.0725024139761223No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG2860.18288666781770163No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC1910.12213759983629724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC4750.056.7368448
CCGTCTT4750.056.7368447
TATGCCG4950.056.5656643
CGTATGC5050.056.13861541
TCGTATG5000.056.040
CTCGTAT4950.055.8585939
ATGCCGT4950.055.8585944
TGCCGTC5050.055.44554545
GCCGTCT5000.055.346
CTTGAAA4850.054.8453657
TCTCGTA4500.054.44444738
ATCTCGT4550.053.84615737
CTGCTTG5050.053.36633754
GTCTTCT5050.053.36633749
GTATGCC5250.053.33333242
TCTTCTG5200.053.17307750
GCTAGTT200.006940864552.558
TATACAG200.006940864552.55
ATCGCCT200.006940864552.535
GGCTAGT200.006940864552.557
Produced by FastQC (version 0.11.5)