##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781125_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156381
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.32525690461117	34.0	34.0	34.0	33.0	34.0
2	33.315153375410056	34.0	34.0	34.0	33.0	34.0
3	33.35250446026052	34.0	34.0	34.0	33.0	34.0
4	33.4156323338513	34.0	34.0	34.0	34.0	34.0
5	33.40350809880996	34.0	34.0	34.0	34.0	34.0
6	36.84356155798978	38.0	37.0	38.0	34.0	38.0
7	36.7585832038419	38.0	37.0	38.0	34.0	38.0
8	36.5390168882409	38.0	37.0	38.0	34.0	38.0
9	36.494753198918026	38.0	37.0	38.0	34.0	38.0
10-11	36.40922810315831	38.0	37.0	38.0	34.0	38.0
12-13	36.344671027810286	38.0	37.0	38.0	34.0	38.0
14-15	36.36343289785844	38.0	37.0	38.0	34.0	38.0
16-17	36.30537277546505	38.0	37.0	38.0	34.0	38.0
18-19	36.322849323127485	38.0	37.0	38.0	34.0	38.0
20-21	36.253710489125915	38.0	37.0	38.0	34.0	38.0
22-23	36.2981404390559	38.0	37.0	38.0	34.0	38.0
24-25	36.28134172309935	38.0	37.0	38.0	34.0	38.0
26-27	36.0860078909842	38.0	37.0	38.0	33.0	38.0
28-29	36.063904822197074	38.0	37.0	38.0	32.0	38.0
30-31	36.12389292816902	38.0	37.0	38.0	33.5	38.0
32-33	36.03412818692808	38.0	37.0	38.0	32.0	38.0
34-35	36.0406379291602	38.0	37.0	38.0	32.0	38.0
36-37	35.99565804029901	38.0	37.0	38.0	32.0	38.0
38-39	35.90456641152058	38.0	37.0	38.0	32.0	38.0
40-41	35.97372762675773	38.0	37.0	38.0	32.0	38.0
42-43	36.010356117431144	38.0	37.0	38.0	32.0	38.0
44-45	35.97270768187951	38.0	37.0	38.0	32.0	38.0
46-47	35.99071178723758	38.0	37.0	38.0	32.0	38.0
48-49	35.96129005441837	38.0	37.0	38.0	32.0	38.0
50-51	35.867659754062196	38.0	36.5	38.0	31.5	38.0
52-53	35.88029875752169	38.0	37.0	38.0	31.5	38.0
54-55	35.906986782281734	38.0	37.0	38.0	32.0	38.0
56-57	35.866783688555515	38.0	37.0	38.0	32.0	38.0
58-59	35.83419021492381	38.0	36.5	38.0	31.0	38.0
60-61	35.83541798556091	38.0	36.5	38.0	31.5	38.0
62-63	35.79903249115941	38.0	36.0	38.0	31.0	38.0
64-65	35.756974952200075	38.0	36.0	38.0	31.0	38.0
66-67	35.70934128826392	38.0	36.0	38.0	31.0	38.0
68-69	35.61378300432917	38.0	36.0	38.0	31.0	38.0
70-71	35.438764939474744	38.0	36.0	38.0	30.0	38.0
72-73	35.30817362723093	38.0	36.0	38.0	29.0	38.0
74-75	35.300404141168045	38.0	36.0	38.0	28.5	38.0
76	34.395776980579484	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	8.0
22	22.0
23	41.0
24	126.0
25	236.0
26	494.0
27	805.0
28	1330.0
29	2004.0
30	3182.0
31	4781.0
32	6661.0
33	9143.0
34	12831.0
35	17798.0
36	26758.0
37	70160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32623528433761	18.28227214303528	10.465465753512255	27.926026819114853
2	23.3436287016965	19.475511730964758	31.979588313158246	25.201271254180497
3	20.164853786585326	21.483428293718546	28.960679366419196	29.391038553276932
4	14.718539976084053	17.909464704791503	34.864209846464725	32.50778547265972
5	14.579136851663566	33.23613482456309	35.75626194998114	16.428466373792215
6	31.212871128845578	37.2941725657209	16.730932785952255	14.762023519481268
7	30.044570631982147	27.56409026672038	20.77426285801984	21.617076243277637
8	26.411776366694163	35.03622562843312	18.041194262730126	20.51080374214259
9	24.63214840677576	15.95846042677819	20.09834954374253	39.31104162270353
10-11	25.671596933131262	24.94580543672185	26.462294012699754	22.920303617447132
12-13	25.59262314475544	22.056707656300958	26.31521732179741	26.035451877146198
14-15	23.52875349307141	23.14379624123135	22.461808020155903	30.865642245541338
16-17	25.207346160978634	26.86611544880772	23.910513425544025	24.016024964669622
18-19	24.79009598352741	25.60797027771916	24.88153931743626	24.720394421317167
20-21	23.408854016792322	25.340994110537725	24.71304058677205	26.5371112858979
22-23	23.823546338749594	26.454940178154633	24.90775733624929	24.813756146846483
24-25	24.706326216100422	26.66660272027932	24.808640435858575	23.818430627761686
26-27	24.029773437949622	27.03301551978821	25.277687187062366	23.6595238551998
28-29	23.56967918097467	26.314258125987173	25.769434905774997	24.34662778726316
30-31	23.608366745320723	26.52560093617511	25.12709344485583	24.738938873648333
32-33	24.45181959445201	25.796931852335003	24.79137491127439	24.9598736419386
34-35	23.828022585864012	25.970226562050374	25.16162449402421	25.0401263580614
36-37	23.696932491798876	26.362857380372297	26.25063147057507	23.689578657253758
38-39	23.037325506295524	25.510452037012172	26.95532065915936	24.49690179753295
40-41	23.147952756409026	24.374764197696653	27.417972771628268	25.059310274266057
42-43	24.429438358879914	24.44862227508457	27.044525869511006	24.077413496524514
44-45	22.39146699407217	25.730107877555458	27.753371573272968	24.125053555099406
46-47	23.979895255817524	24.128890338340337	26.479879269220685	25.411335136621453
48-49	23.19047710399601	24.21074171414686	26.908959528331444	25.689821653525684
50-51	21.96558405432885	24.27724595698966	28.11083187855302	25.646338110128468
52-53	21.672070136397643	24.140400688063128	29.686470862828607	24.501058312710626
54-55	20.86890351129613	24.30058638837199	28.768200740499168	26.062309359832714
56-57	22.092837365153056	24.266375071140356	27.349869869101745	26.290917694604843
58-59	20.963544164572422	24.4163293494734	29.09464704791503	25.52547943803915
60-61	21.918583459627445	25.40941674500099	27.032695787851463	25.639304007520096
62-63	19.924415370153664	27.03941015852309	26.68834449197793	26.347829979345317
64-65	20.61311796190074	27.510055569410607	25.662644438902426	26.214182029786226
66-67	20.678023545059823	27.142044110217995	25.187203048963745	26.992729295758437
68-69	20.368203298354658	27.654894136755743	24.90807706818603	27.068825496703564
70-71	20.767548487348208	27.10431574168217	24.80672204423811	27.321413726731507
72-73	21.06138213721616	25.870150465849434	24.78242241704555	28.286044979888864
74-75	21.175846170570594	26.067105338883877	24.366131435404554	28.390917055140967
76	21.274323607087815	26.295393941719265	24.128250874466847	28.302031576726073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	5.0
15	10.5
16	13.0
17	14.0
18	24.0
19	40.0
20	69.0
21	92.0
22	100.0
23	155.5
24	263.0
25	323.0
26	384.5
27	496.0
28	732.0
29	918.0
30	1041.0
31	1304.0
32	1882.0
33	2320.0
34	2546.0
35	3148.0
36	4005.0
37	4486.0
38	4969.5
39	6164.5
40	7623.5
41	8871.0
42	9371.0
43	9796.5
44	10134.0
45	10074.0
46	10102.0
47	9794.0
48	8877.0
49	8009.0
50	7750.0
51	7064.5
52	5814.5
53	4942.5
54	4635.0
55	4235.5
56	3514.0
57	2847.5
58	2503.0
59	2362.5
60	2141.0
61	1942.5
62	1825.0
63	1789.5
64	1759.5
65	1747.5
66	1732.5
67	1735.0
68	1728.5
69	1647.5
70	1526.5
71	1480.0
72	1452.5
73	1393.0
74	1269.0
75	1177.0
76	1091.5
77	944.5
78	783.5
79	684.0
80	610.5
81	461.5
82	338.0
83	290.0
84	245.5
85	181.0
86	121.0
87	81.0
88	73.0
89	47.5
90	23.5
91	10.0
92	3.0
93	4.0
94	2.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	156381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.62218555962681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.40838371866233	25.747373402139644
2	19.55050785411009	16.665707470856432
3	7.722083027020539	9.873961670535424
4	3.5512280017403564	6.054443954188808
5	1.9128921428892924	4.076582193488979
6	1.3067678874169204	3.3418382028507296
7	0.9121869983346585	2.721558245566917
8	0.7261488605164058	2.4760041181473453
9	0.5866202571527164	2.2502733708059166
>10	3.2751714101390785	23.210620216010895
>50	0.04200861176541191	1.10563303726156
>100	0.004500922689151276	0.40350170417122283
>500	0.0	0.0
>1k	0.0015003075630504253	2.0725024139761223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT	3241	2.0725024139761223	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG	286	0.18288666781770163	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC	191	0.12213759983629724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001918391620465402	0.0
2	0.0	0.0	0.0	0.001918391620465402	0.0
3	0.0	0.0	0.0	0.001918391620465402	0.0
4	0.0	0.0	0.0	0.004476247114419271	0.0
5	0.0	0.0	0.0	0.005115710987907738	0.0
6	0.0	0.0	0.0	0.005115710987907738	0.0
7	0.0	0.0	0.0	0.005115710987907738	0.0
8	0.0	0.0	0.0	0.005115710987907738	0.0
9	0.0	0.0	0.0	0.0063946387348846724	0.0
10	0.0	0.0	0.0	0.00703410260837314	0.0
11	0.0	0.0	0.0	0.00703410260837314	0.0
12	0.0	0.0	0.0	0.00703410260837314	0.0
13	0.0	0.0	0.0	0.00703410260837314	0.0
14	0.0	0.0	0.0	0.00703410260837314	0.0
15	0.0	0.0	0.0	0.00703410260837314	0.0
16	0.0	0.0	0.0	0.00703410260837314	0.0
17	0.0	0.0	0.0	0.009591958102327009	0.0
18	0.0	0.0	0.0	0.009591958102327009	0.0
19	0.0	0.0	0.0	0.01151034972279241	0.0
20	0.0	0.0	0.0	0.012149813596280878	0.0
21	0.0	0.0	0.0	0.012149813596280878	0.0
22	0.0	0.0	0.0	0.012149813596280878	0.0
23	0.0	0.0	0.0	0.012149813596280878	0.0
24	0.0	0.0	0.0	0.012789277469769345	0.0
25	0.0	0.0	0.0	0.01406820521674628	0.0
26	0.0	0.0	0.0	0.014707669090234746	0.0
27	0.0	0.0	0.0	0.02557855493953869	0.0
28	0.0	0.0	0.0	0.041565151776750374	0.0
29	0.0	0.0012789277469769345	0.0	0.06394638734884672	0.0
30	0.0	0.0012789277469769345	0.0	0.09400118940280469	0.0
31	0.0	0.0012789277469769345	0.0	0.11766135272187797	0.0
32	0.0	0.0012789277469769345	0.0	0.1445188354083936	0.0
33	0.0	0.0012789277469769345	0.0	0.18800237880560938	0.0
34	0.0	0.0012789277469769345	0.0	0.27688785722050635	0.0
35	0.0	0.0012789277469769345	0.0	0.3907124267014535	0.0
36	0.0	0.0012789277469769345	0.0	0.5684833835312474	0.0
37	0.0	0.0012789277469769345	0.0	0.8658340847033846	0.0
38	0.0	0.0012789277469769345	0.0	1.1926001240559914	0.0
39	0.0	0.0012789277469769345	0.0	1.591625581112795	0.0
40	0.0	0.0012789277469769345	0.0	2.0315767260728603	0.0
41	0.0	0.0012789277469769345	0.0	2.524603372532469	0.0
42	0.0	0.0012789277469769345	0.0	3.0732633759855736	0.0
43	0.0	0.0012789277469769345	0.0	3.7351084850461373	0.0
44	0.0	0.0012789277469769345	0.0	4.474968186672294	0.0
45	0.0	0.0012789277469769345	0.0	5.287087306002648	0.0
46	0.0	0.0012789277469769345	0.0	6.133737474501378	0.0
47	0.0	0.0012789277469769345	0.0	6.922196430512658	0.0
48	0.0	0.0012789277469769345	0.0	7.638395968819742	0.0
49	0.0	0.0012789277469769345	0.0	8.417902430602183	0.0
50	0.0	0.0012789277469769345	0.0	9.098931455867401	0.0
51	0.0	0.0012789277469769345	0.0	9.81321260255402	0.0
52	0.0	0.0012789277469769345	0.0	10.553072304180175	0.0
53	0.0	0.0012789277469769345	0.0	11.28525843932447	0.0
54	0.0	0.0012789277469769345	0.0	12.120398258100408	0.0
55	0.0	0.0012789277469769345	0.0	12.966408962725652	0.0
56	0.0	0.0012789277469769345	0.0	13.736323466405766	0.0
57	0.0	0.0012789277469769345	0.0	14.517108855935184	0.0
58	0.0	0.0012789277469769345	0.0	15.237145177483198	0.0
59	0.0	0.0012789277469769345	0.0	15.929684552471208	0.0
60	0.0	0.0012789277469769345	0.0	16.57234574532712	0.0
61	0.0	0.0012789277469769345	0.0	17.261048337074197	0.0
62	0.0	0.0012789277469769345	0.0	17.908825240918013	0.0
63	0.0	0.0012789277469769345	0.0	18.570670349978577	0.0
64	0.0	0.0012789277469769345	0.0	19.294543454767524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	475	0.0	56.73684	48
CCGTCTT	475	0.0	56.73684	47
TATGCCG	495	0.0	56.56566	43
CGTATGC	505	0.0	56.138615	41
TCGTATG	500	0.0	56.0	40
CTCGTAT	495	0.0	55.85859	39
ATGCCGT	495	0.0	55.85859	44
TGCCGTC	505	0.0	55.445545	45
GCCGTCT	500	0.0	55.3	46
CTTGAAA	485	0.0	54.84536	57
TCTCGTA	450	0.0	54.444447	38
ATCTCGT	455	0.0	53.846157	37
CTGCTTG	505	0.0	53.366337	54
GTCTTCT	505	0.0	53.366337	49
GTATGCC	525	0.0	53.333332	42
TCTTCTG	520	0.0	53.173077	50
GCTAGTT	20	0.0069408645	52.5	58
TATACAG	20	0.0069408645	52.5	5
ATCGCCT	20	0.0069408645	52.5	35
GGCTAGT	20	0.0069408645	52.5	57
>>END_MODULE