FastQCFastQC Report
Wed 25 May 2016
SRR1781124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256409
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCG200.00694590952.59
GTACTAG350.00125394540.01
ATCGCGG350.00125394540.030
AATATGG601.2128701E-535.00000452
CCGAAGC652.0976895E-532.30769368
TCCGAAG552.9870178E-431.81818467
GTATAAC552.9870178E-431.8181843
TTCCGAA450.004302281431.11110966
TTTGCAC450.004302281431.11110945
GACCATA604.9736263E-429.1666681
CGAGCTT604.9736263E-429.16666826
CGACGAA755.5713987E-528.070
GTATCCA755.5713987E-528.013
TATTCCG500.007200089327.99999864
GACGATT500.007200089327.99999867
TATCGGT500.007200089327.99999824
TAGCCCG657.942389E-426.9230795
CCAGAAC959.360732E-625.78947351
ATTTAGA700.001223956525.02
CGTGGTT700.001223956525.064
Produced by FastQC (version 0.11.5)