##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781124_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256409
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.582105152315247	34.0	30.0	34.0	12.0	34.0
2	29.578774535995226	34.0	27.0	34.0	12.0	34.0
3	29.417079743690746	34.0	27.0	34.0	11.0	34.0
4	29.704788053461463	34.0	31.0	34.0	11.0	34.0
5	29.761677632220398	34.0	31.0	34.0	11.0	34.0
6	31.859930813660988	36.0	31.0	38.0	11.0	38.0
7	31.793162486496183	36.0	27.0	38.0	12.0	38.0
8	31.43019160793888	36.0	27.0	38.0	11.0	38.0
9	32.10803052934959	37.0	31.0	38.0	12.0	38.0
10-11	31.837868405555188	36.5	30.0	38.0	11.0	38.0
12-13	31.80492299412267	36.0	31.0	38.0	11.0	38.0
14-15	31.68929327753706	36.0	27.5	38.0	11.0	38.0
16-17	31.644907160045086	36.0	27.5	38.0	11.0	38.0
18-19	31.703998299591667	36.0	28.5	38.0	11.0	38.0
20-21	31.62613051803954	36.0	27.5	38.0	11.0	38.0
22-23	31.733002741713435	36.0	29.0	38.0	11.0	38.0
24-25	31.727400364261783	36.0	28.5	38.0	11.0	38.0
26-27	31.63875877991802	36.0	27.0	38.0	11.0	38.0
28-29	31.74963047318932	36.0	28.5	38.0	11.0	38.0
30-31	31.811397025845427	36.0	30.0	38.0	11.0	38.0
32-33	31.782575884621835	36.0	29.0	38.0	11.0	38.0
34-35	31.796430702510442	36.0	30.0	38.0	11.0	38.0
36-37	31.612597451727513	36.0	27.0	38.0	11.0	38.0
38-39	31.62816437800545	36.0	27.0	38.0	11.0	38.0
40-41	31.619266484405774	36.0	27.0	38.0	11.0	38.0
42-43	31.717570366094794	36.0	28.0	38.0	11.0	38.0
44-45	31.725920307009503	36.0	28.5	38.0	11.0	38.0
46-47	31.683421798766815	36.0	28.0	38.0	11.0	38.0
48-49	31.648313826737752	36.0	27.0	38.0	11.0	38.0
50-51	31.477251188530822	36.0	27.0	38.0	11.0	38.0
52-53	31.580740145626713	36.0	27.0	38.0	11.0	38.0
54-55	31.51390746814659	36.0	27.0	38.0	11.0	38.0
56-57	31.4908603052155	36.0	27.0	38.0	11.0	38.0
58-59	31.50561017748987	36.0	27.0	38.0	11.0	38.0
60-61	31.446201186385814	36.0	27.0	38.0	11.0	38.0
62-63	31.42774629595685	36.0	27.0	38.0	11.0	38.0
64-65	31.45600193440948	36.0	27.0	38.0	11.0	38.0
66-67	31.320441170161736	36.0	27.0	38.0	11.0	38.0
68-69	31.053516062228706	36.0	27.0	38.0	11.0	38.0
70-71	31.218449820404118	36.0	27.0	38.0	11.0	38.0
72-73	31.168196124161007	36.0	27.0	38.0	11.0	38.0
74-75	31.147980375103838	36.0	27.0	38.0	11.0	38.0
76	29.648358676957518	34.0	24.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	20.0
14	76.0
15	301.0
16	766.0
17	1688.0
18	2836.0
19	3944.0
20	4915.0
21	5569.0
22	6305.0
23	6909.0
24	7631.0
25	8166.0
26	8785.0
27	9702.0
28	10503.0
29	11344.0
30	12247.0
31	13245.0
32	14309.0
33	16176.0
34	18574.0
35	22053.0
36	29417.0
37	40924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.73272389034706	17.669036578279236	9.337425753386192	25.26081377798751
2	24.53346021395505	20.691941390512813	30.147537722934842	24.627060672597295
3	20.009438046246427	21.266414205429605	29.355443841674823	29.36870390664914
4	14.157459371550921	17.302044780019422	36.035396573443215	32.50509927498645
5	13.784227542714959	32.90992125861417	36.392248322016776	16.913602876654096
6	28.79267108408831	36.55292910935263	19.61982613714807	15.03457366941098
7	28.558279935571683	29.183452998919694	22.76714155899364	19.49112550651498
8	25.633265603001455	33.68329504814574	20.047268231614336	20.636171117238476
9	24.607170575135818	16.75838211607237	21.556185625309567	37.07826168348225
10-11	24.81504159370381	24.50401506967384	27.883576629525486	22.797366707096863
12-13	25.095062965808534	22.77104157810373	27.110007839038413	25.023887617049322
14-15	23.273754041394803	23.925642235646954	24.447269791621977	28.353333931336262
16-17	23.55475041827705	26.899016805182345	25.13152814448791	24.414704632052697
18-19	22.693626198768374	26.659945633733606	26.720395929940054	23.926032237557965
20-21	23.02668783077037	26.632840500918455	26.688220772281785	23.65225089602939
22-23	23.215838757609912	26.245178601375148	26.814581391448815	23.724401249566125
24-25	22.888237152362045	26.42730949381652	26.694850804768944	23.989602549052492
26-27	22.390009711047583	26.47274471644911	27.121122893502182	24.01612267900113
28-29	22.852746978460196	26.284373793431588	27.696180711285486	23.16669851682273
30-31	22.818816812202382	26.538460038454186	27.112737852415474	23.529985296927954
32-33	22.52651037990086	26.731900986314834	27.032397458747546	23.709191175036757
34-35	23.489230097227477	26.01117745476953	27.055602572452603	23.44398987555039
36-37	23.342199376776946	26.08001279206268	26.77090117741577	23.806886653744602
38-39	22.646825969447253	26.58389526108678	27.28999372096923	23.479285048496738
40-41	22.758561516951435	25.90080691395388	27.58503016664782	23.75560140244687
42-43	22.563560561446753	26.065972723266345	27.311638827030254	24.058827888256655
44-45	22.162638596929124	25.844061635902015	27.56299505867579	24.430304708493072
46-47	22.495505227975617	25.222983592619602	27.841846425047486	24.439664754357295
48-49	21.69054128365229	25.442944670428886	28.46994450272806	24.39656954319076
50-51	21.963542621358844	25.514315020143595	28.176273063738012	24.345869294759545
52-53	21.464925178133374	25.278948866849447	28.720910732462592	24.53521522255459
54-55	21.05464316775152	25.845231641635042	28.60605516967033	24.494070020943106
56-57	20.91607906149574	25.684456023213002	28.427350160681414	24.97211475460984
58-59	20.991154722161557	25.46761411500421	28.4542603974915	25.086970765342738
60-61	20.905508202731188	25.47945953429781	28.47663006491594	25.138402198055054
62-63	20.622211475460983	25.322142834856948	28.727457801628653	25.328187888053417
64-65	21.126639717482064	25.52976987892367	28.06985727852236	25.273733125071907
66-67	21.038150135721196	25.334037939533864	27.751084209541045	25.876727715203895
68-69	20.86674362734392	25.448503946834734	27.40495616361424	26.279796262207107
70-71	21.40046332407725	25.20338678980375	27.475156781379678	25.92099310473932
72-73	21.24017191351284	24.920049296433806	27.247004773642008	26.592774016411347
74-75	21.522144394870672	24.70262238307697	27.212294468191324	26.562938753861033
76	21.74338654259406	24.295949050150345	26.845781544329565	27.114882862926027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	3.0
12	5.0
13	5.0
14	11.5
15	29.0
16	60.0
17	80.0
18	99.5
19	151.0
20	240.0
21	297.0
22	355.0
23	504.5
24	735.0
25	874.0
26	1093.5
27	1496.0
28	1894.5
29	2110.0
30	2410.0
31	3105.5
32	4036.0
33	4571.0
34	4953.5
35	6129.0
36	7506.0
37	8090.0
38	9096.0
39	10867.0
40	12474.0
41	14034.0
42	14752.0
43	15043.0
44	15490.0
45	15625.5
46	15605.0
47	15287.0
48	14404.5
49	13310.5
50	12781.0
51	11947.5
52	10242.0
53	8713.5
54	8057.0
55	7482.0
56	6489.0
57	5461.5
58	4852.0
59	4452.5
60	3737.0
61	3211.5
62	3002.0
63	2826.0
64	2504.0
65	2273.5
66	2081.0
67	1973.0
68	1914.5
69	1787.5
70	1635.5
71	1552.0
72	1488.0
73	1378.5
74	1281.0
75	1229.0
76	1124.5
77	935.5
78	787.0
79	723.0
80	633.5
81	477.0
82	348.0
83	286.0
84	252.0
85	177.5
86	121.0
87	105.0
88	81.5
89	48.5
90	25.5
91	13.5
92	15.0
93	11.5
94	6.5
95	3.5
96	2.0
97	1.5
98	0.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	3.9000191100936393E-4
58-59	3.9000191100936393E-4
60-61	1.9500095550468196E-4
62-63	3.9000191100936393E-4
64-65	1.9500095550468196E-4
66-67	3.9000191100936393E-4
68-69	3.9000191100936393E-4
70-71	3.9000191100936393E-4
72-73	3.9000191100936393E-4
74-75	3.9000191100936393E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	256409.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.39781311886638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.17999968144312	55.22970074075825
2	9.03905018196205	12.003463295079426
3	3.05273096769414	6.0808398088539475
4	1.3416193472553846	3.5632236278287404
5	0.7442109032229949	2.470698823661158
6	0.4880416115787403	1.9442937431901341
7	0.37537037212749313	1.7446640273216432
8	0.28471845266669993	1.5123746089325076
9	0.2580262173022214	1.5419138900580815
>10	1.2233747813003952	13.383020433832387
>50	0.012857483446704863	0.5258070004837379
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011700057330280918	0.0
2	0.0	0.0	0.0	0.0011700057330280918	0.0
3	0.0	0.0	0.0	0.0011700057330280918	0.0
4	0.0	0.0	0.0	0.0023400114660561836	0.0
5	0.0	0.0	0.0	0.0023400114660561836	0.0
6	0.0	0.0	0.0	0.0023400114660561836	0.0
7	0.0	0.0	0.0	0.0023400114660561836	0.0
8	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	0.0
9	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	0.0
10	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	0.0
11	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	0.0
12	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	0.0
13	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
14	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
15	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
16	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
17	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
18	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
19	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
20	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
21	0.0	3.9000191100936393E-4	0.0	0.0023400114660561836	3.9000191100936393E-4
22	0.0	3.9000191100936393E-4	0.0	0.0031200152880749114	3.9000191100936393E-4
23	0.0	3.9000191100936393E-4	0.0	0.0031200152880749114	3.9000191100936393E-4
24	0.0	3.9000191100936393E-4	0.0	0.0035100171990842754	3.9000191100936393E-4
25	0.0	3.9000191100936393E-4	0.0	0.004290021021103004	3.9000191100936393E-4
26	0.0	3.9000191100936393E-4	0.0	0.0050700248431217315	3.9000191100936393E-4
27	0.0	3.9000191100936393E-4	0.0	0.010140049686243463	3.9000191100936393E-4
28	0.0	3.9000191100936393E-4	0.0	0.01677008217340265	3.9000191100936393E-4
29	0.0	3.9000191100936393E-4	0.0	0.02301011274955247	3.9000191100936393E-4
30	0.0	3.9000191100936393E-4	0.0	0.03159015479175848	3.9000191100936393E-4
31	0.0	3.9000191100936393E-4	0.0	0.04056019874497385	3.9000191100936393E-4
32	0.0	3.9000191100936393E-4	0.0	0.051870254164245407	3.9000191100936393E-4
33	0.0	3.9000191100936393E-4	0.0	0.0756603707358166	3.9000191100936393E-4
34	0.0	3.9000191100936393E-4	0.0	0.10530051597252826	3.9000191100936393E-4
35	0.0	3.9000191100936393E-4	0.0	0.15366075293768938	3.9000191100936393E-4
36	0.0	3.9000191100936393E-4	0.0	0.23322114278359965	3.9000191100936393E-4
37	0.0	3.9000191100936393E-4	0.0	0.37128181928091447	3.9000191100936393E-4
38	0.0	3.9000191100936393E-4	0.0	0.5495126926121938	3.9000191100936393E-4
39	0.0	3.9000191100936393E-4	0.0	0.7605037264682597	3.9000191100936393E-4
40	0.0	3.9000191100936393E-4	0.0	1.0089349437812245	3.9000191100936393E-4
41	0.0	3.9000191100936393E-4	0.0	1.2920763311740227	3.9000191100936393E-4
42	0.0	3.9000191100936393E-4	0.0	1.616947923044823	3.9000191100936393E-4
43	0.0	3.9000191100936393E-4	0.0	2.0022698111220745	3.9000191100936393E-4
44	0.0	3.9000191100936393E-4	0.0	2.482752165485611	3.9000191100936393E-4
45	0.0	3.9000191100936393E-4	0.0	3.0205648007675236	3.9000191100936393E-4
46	0.0	3.9000191100936393E-4	0.0	3.5782675335109144	3.9000191100936393E-4
47	0.0	3.9000191100936393E-4	0.0	4.176140463088269	3.9000191100936393E-4
48	0.0	3.9000191100936393E-4	0.0	4.729943176721566	3.9000191100936393E-4
49	0.0	3.9000191100936393E-4	0.0	5.3223560795447895	3.9000191100936393E-4
50	0.0	3.9000191100936393E-4	0.0	5.847688653674403	3.9000191100936393E-4
51	0.0	3.9000191100936393E-4	0.0	6.35235112652052	3.9000191100936393E-4
52	0.0	3.9000191100936393E-4	0.0	6.876513694917105	3.9000191100936393E-4
53	0.0	3.9000191100936393E-4	0.0	7.381956171585241	3.9000191100936393E-4
54	0.0	3.9000191100936393E-4	0.0	7.943558923438725	3.9000191100936393E-4
55	0.0	3.9000191100936393E-4	0.0	8.55391191416838	3.9000191100936393E-4
56	0.0	3.9000191100936393E-4	0.0	9.188445023380615	3.9000191100936393E-4
57	0.0	3.9000191100936393E-4	0.0	9.780467924292829	3.9000191100936393E-4
58	0.0	3.9000191100936393E-4	0.0	10.410711012483961	3.9000191100936393E-4
59	0.0	3.9000191100936393E-4	0.0	10.990253852243876	3.9000191100936393E-4
60	0.0	3.9000191100936393E-4	0.0	11.550296596453322	3.9000191100936393E-4
61	0.0	3.9000191100936393E-4	0.0	12.088889235557254	3.9000191100936393E-4
62	0.0	3.9000191100936393E-4	0.0	12.628651880394214	3.9000191100936393E-4
63	0.0	3.9000191100936393E-4	0.0	13.130974341774275	3.9000191100936393E-4
64	0.0	3.9000191100936393E-4	0.0	13.62393675729011	3.9000191100936393E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACCG	20	0.006945909	52.5	9
GTACTAG	35	0.001253945	40.0	1
ATCGCGG	35	0.001253945	40.0	30
AATATGG	60	1.2128701E-5	35.000004	52
CCGAAGC	65	2.0976895E-5	32.307693	68
TCCGAAG	55	2.9870178E-4	31.818184	67
GTATAAC	55	2.9870178E-4	31.818184	3
TTCCGAA	45	0.0043022814	31.111109	66
TTTGCAC	45	0.0043022814	31.111109	45
GACCATA	60	4.9736263E-4	29.166668	1
CGAGCTT	60	4.9736263E-4	29.166668	26
CGACGAA	75	5.5713987E-5	28.0	70
GTATCCA	75	5.5713987E-5	28.0	13
TATTCCG	50	0.0072000893	27.999998	64
GACGATT	50	0.0072000893	27.999998	67
TATCGGT	50	0.0072000893	27.999998	24
TAGCCCG	65	7.942389E-4	26.923079	5
CCAGAAC	95	9.360732E-6	25.789473	51
ATTTAGA	70	0.0012239565	25.0	2
CGTGGTT	70	0.0012239565	25.0	64
>>END_MODULE