FastQCFastQC Report
Wed 25 May 2016
SRR1781124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232480
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT22150.9527701307639367No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG2640.11355815554026152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT3350.056.4179147
CGTCTTC3400.055.58823448
TCGTATG3550.054.22535340
CTCGTAT3450.053.76811639
CGTATGC3650.053.69862741
TATGCCG3750.053.243
AACCGAG200.00694509752.546
TACGGAG200.00694509752.552
CTACGGA200.00694509752.551
GCCGTCT3800.050.6578946
TGCCGTC3950.050.50633245
ATCTCGT2950.049.8305137
GTATGCC3950.049.62025542
ATGCCGT4050.049.2592644
TCTCGTA3000.049.00000438
CATCTCG3050.047.0491836
GTCTTCT3950.046.9620349
CTGCGTT305.869674E-446.66666823
CTTGAAA4250.044.4705957
TCTATCG404.583447E-543.7529
Produced by FastQC (version 0.11.5)