##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781124_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	232480
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.282656572608396	34.0	34.0	34.0	32.0	34.0
2	33.270130763936685	34.0	34.0	34.0	33.0	34.0
3	33.31196662078458	34.0	34.0	34.0	33.0	34.0
4	33.38038540949759	34.0	34.0	34.0	33.0	34.0
5	33.36743805918789	34.0	34.0	34.0	33.0	34.0
6	36.77025550584997	38.0	37.0	38.0	34.0	38.0
7	36.6851083964212	38.0	37.0	38.0	34.0	38.0
8	36.48574931176876	38.0	37.0	38.0	34.0	38.0
9	36.444666207845835	38.0	37.0	38.0	34.0	38.0
10-11	36.346795423262215	38.0	37.0	38.0	34.0	38.0
12-13	36.28636871988988	38.0	37.0	38.0	34.0	38.0
14-15	36.28999053682037	38.0	37.0	38.0	34.0	38.0
16-17	36.22899819339298	38.0	37.0	38.0	34.0	38.0
18-19	36.23847427735719	38.0	37.0	38.0	34.0	38.0
20-21	36.18342222986924	38.0	37.0	38.0	33.5	38.0
22-23	36.22794864074329	38.0	37.0	38.0	34.0	38.0
24-25	36.234585770818995	38.0	37.0	38.0	34.0	38.0
26-27	36.0022862181693	38.0	37.0	38.0	32.0	38.0
28-29	35.98020259807295	38.0	37.0	38.0	32.0	38.0
30-31	36.008325447350316	38.0	37.0	38.0	32.0	38.0
32-33	35.98387603234687	38.0	37.0	38.0	32.0	38.0
34-35	35.975954920853404	38.0	37.0	38.0	32.0	38.0
36-37	35.935274002064695	38.0	37.0	38.0	32.0	38.0
38-39	35.87083189951824	38.0	36.5	38.0	31.5	38.0
40-41	35.90214642119752	38.0	37.0	38.0	32.0	38.0
42-43	35.916386355815554	38.0	36.5	38.0	32.0	38.0
44-45	35.89221223331039	38.0	36.0	38.0	32.0	38.0
46-47	35.893928509979354	38.0	36.5	38.0	31.5	38.0
48-49	35.87600653819683	38.0	36.0	38.0	31.5	38.0
50-51	35.79104654163799	38.0	36.0	38.0	31.0	38.0
52-53	35.81117300412939	38.0	36.0	38.0	31.0	38.0
54-55	35.83425240880936	38.0	36.0	38.0	31.0	38.0
56-57	35.78209738472127	38.0	36.0	38.0	31.0	38.0
58-59	35.77419778045423	38.0	36.0	38.0	31.0	38.0
60-61	35.764448554714384	38.0	36.0	38.0	31.0	38.0
62-63	35.7423434273916	38.0	36.0	38.0	31.0	38.0
64-65	35.70037852718514	38.0	36.0	38.0	31.0	38.0
66-67	35.679406830695115	38.0	36.0	38.0	31.0	38.0
68-69	35.616425068823126	38.0	36.0	38.0	31.0	38.0
70-71	35.539910960082594	38.0	36.0	38.0	31.0	38.0
72-73	35.48162422573985	38.0	36.0	38.0	31.0	38.0
74-75	35.483581383344806	38.0	36.0	38.0	31.0	38.0
76	34.58778389538885	37.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	12.0
22	35.0
23	89.0
24	147.0
25	353.0
26	653.0
27	1183.0
28	1929.0
29	3209.0
30	4824.0
31	7336.0
32	10263.0
33	14252.0
34	19414.0
35	26798.0
36	40765.0
37	101216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.74681693048864	18.010581555402617	9.301015141087406	25.941586373021337
2	24.178854094975915	21.138592567102545	29.969029593943564	24.713523743977976
3	20.443048864418444	21.760151410874055	29.815467997247076	27.98133172746043
4	14.475223675154853	18.04370268410186	36.06589814177564	31.415175498967653
5	13.817532690984171	33.46223331039229	36.08568479008947	16.634549208534068
6	29.53759463179628	37.86003097040606	18.42309015829319	14.179284239504472
7	28.87044046799725	29.80858568479009	21.48700963523744	19.833964211975225
8	25.294218857536134	35.704576737783896	19.433499655884376	19.567704748795595
9	24.73632140399174	17.01780798348245	20.679198210598763	37.56667240192705
10-11	24.68513420509291	25.454662766689605	27.41762732278045	22.442575705437026
12-13	25.357450103234687	23.007140399174123	26.634979353062626	25.00043014452856
14-15	23.058542670337236	23.969373709566412	23.68784411562285	29.284239504473504
16-17	23.93044562973159	27.62581727460427	24.670294218857535	23.77344287680661
18-19	23.509119064005507	26.694339298004127	25.997935306262903	23.79860633172746
20-21	22.687973158981418	26.914143152099108	25.88781830695114	24.510065381968342
22-23	22.956383344803854	27.068780110116997	26.0663282863042	23.908508258774948
24-25	23.65579834824501	27.049208534067446	25.950404335856845	23.344588781830694
26-27	22.85637474191328	27.38106503785272	26.247849277357194	23.514710942876807
28-29	23.30329490708878	26.507441500344115	26.88811940812113	23.301144184445974
30-31	22.990794907088784	27.690554026152785	26.420767377838956	22.897883688919478
32-33	22.28987439779766	27.670767377838956	26.829189607708194	23.210168616655196
34-35	23.90635753613214	26.12590330350998	26.40614246386786	23.56159669649002
36-37	22.977460426703374	26.69197350309704	26.067833792154165	24.262732278045423
38-39	22.48494494150034	26.81370440467997	27.166637990364762	23.534712663454922
40-41	22.473976256022024	25.75404335856848	27.80475739848589	23.967222986923606
42-43	23.173606331727463	25.99879559532003	27.306865106675843	23.520732966276668
44-45	22.20922229869236	26.646163110805233	27.490106675843084	23.654507914659327
46-47	22.894012388162423	25.486708534067446	27.291379903647627	24.327899174122507
48-49	22.23331039229181	25.48025636613902	27.6288282863042	24.65760495526497
50-51	21.717567102546457	25.462620440467997	28.33813661390227	24.481675843083277
52-53	21.241397109428767	25.498752580867173	29.32897453544391	23.93087577426015
54-55	20.83598589125946	25.66263764624914	28.7880677907777	24.713308671713698
56-57	21.1906400550585	25.675972126634548	27.99595664143152	25.13743117687543
58-59	20.58671713695802	25.599191328286302	28.894098417068136	24.919993117687543
60-61	21.130419821059874	26.01793702684102	27.81852202339986	25.033121128699243
62-63	19.809876118375776	26.72681520991053	27.944339298004127	25.518969373709567
64-65	20.352288368891948	26.9018840330351	27.17653131452168	25.569296283551274
66-67	20.216577770130765	26.81284411562285	26.756065037852718	26.21451307639367
68-69	20.293358568479007	27.145560908465242	26.261398830006883	26.299681693048864
70-71	20.704791810048174	26.841018582243635	26.02352890571232	26.430660701995873
72-73	20.66607880247763	26.364203372333105	25.930617687543016	27.039100137646248
74-75	20.720707157604956	26.641861665519617	25.791035788024775	26.846395388850652
76	21.25043014452856	26.706813489332415	25.487353750860287	26.555402615278734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	5.0
14	7.0
15	14.5
16	37.5
17	51.0
18	84.5
19	140.0
20	235.0
21	308.0
22	367.0
23	527.0
24	728.0
25	828.0
26	1021.0
27	1384.5
28	1745.5
29	1936.0
30	2183.5
31	2684.0
32	3592.5
33	4248.0
34	4599.0
35	5445.5
36	6675.5
37	7410.0
38	8171.5
39	9787.5
40	11506.5
41	12984.0
42	13597.0
43	14132.0
44	14831.0
45	14606.0
46	14217.0
47	14033.5
48	13146.0
49	12047.0
50	11652.0
51	10883.5
52	9115.0
53	7613.0
54	7111.0
55	6527.5
56	5531.0
57	4617.0
58	4116.0
59	3858.5
60	3191.5
61	2613.0
62	2444.0
63	2301.5
64	2133.0
65	1974.5
66	1841.0
67	1840.0
68	1706.5
69	1539.0
70	1495.5
71	1486.0
72	1449.0
73	1333.5
74	1235.5
75	1216.0
76	1129.5
77	919.5
78	716.0
79	636.0
80	593.5
81	474.0
82	345.0
83	293.0
84	245.0
85	154.5
86	90.0
87	68.0
88	58.0
89	35.0
90	16.5
91	9.5
92	8.0
93	5.5
94	2.0
95	3.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	232480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.59682967150872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.42827047775204	26.949092858401663
2	20.876370178037813	18.620398499786333
3	7.8437049072883696	10.494131152317495
4	3.279763939494524	5.850682950895749
5	1.7344151837548307	3.867470926479634
6	1.0452217691904295	2.7968146325723158
7	0.7859097628228457	2.4534358680850388
8	0.6033950790129681	2.1527606050694312
9	0.46177176218486893	1.8534200962743927
>10	2.8952989685196187	22.538157641671024
>50	0.04089123586109415	1.1359815009384187
>100	0.00398938886449699	0.30245396266175595
>500	0.0	0.0
>1k	9.973472161242474E-4	0.9851993048467492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	2215	0.9527701307639367	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	264	0.11355815554026152	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.602890571231934E-4	0.0
2	0.0	0.0	0.0	8.602890571231934E-4	0.0
3	0.0	0.0	0.0	8.602890571231934E-4	0.0
4	0.0	0.0	0.0	0.00258086717136958	0.0
5	0.0	0.0	0.0	0.00258086717136958	0.0
6	0.0	0.0	0.0	0.00258086717136958	0.0
7	0.0	0.0	0.0	0.00258086717136958	0.0
8	0.0	0.0	0.0	0.00258086717136958	0.0
9	0.0	0.0	0.0	0.00258086717136958	0.0
10	0.0	0.0	0.0	0.00258086717136958	0.0
11	0.0	0.0	0.0	0.00258086717136958	0.0
12	0.0	0.0	0.0	0.00258086717136958	0.0
13	0.0	0.0	0.0	0.00258086717136958	0.0
14	0.0	0.0	0.0	0.00258086717136958	0.0
15	0.0	0.0	0.0	0.00258086717136958	0.0
16	0.0	0.0	0.0	0.00258086717136958	0.0
17	0.0	0.0	0.0	0.003011011699931177	0.0
18	0.0	0.0	0.0	0.003011011699931177	0.0
19	0.0	0.0	0.0	0.003011011699931177	0.0
20	0.0	0.0	0.0	0.003011011699931177	0.0
21	0.0	0.0	0.0	0.003011011699931177	0.0
22	0.0	0.0	0.0	0.0034411562284927736	0.0
23	0.0	0.0	0.0	0.0034411562284927736	0.0
24	0.0	0.0	0.0	0.0038713007570543704	0.0
25	0.0	0.0	0.0	0.005591878871300757	0.0
26	0.0	0.0	0.0	0.007312456985547144	0.0
27	0.0	0.0	0.0	0.013764624913971095	0.0
28	0.0	0.0	0.0	0.024518238128011013	0.0
29	0.0	0.0	0.0	0.03527185134205093	0.0
30	0.0	0.0	0.0	0.05032690984170681	0.0
31	0.0	0.0	0.0	0.06409153475567791	0.0
32	0.0	0.0	0.0	0.077856159669649	0.0
33	0.0	0.0	0.0	0.10624569855471439	0.0
34	0.0	0.0	0.0	0.14839986235375086	0.0
35	0.0	0.0	0.0	0.21808327598072952	0.0
36	0.0	0.0	0.0	0.33508258774948385	0.0
37	0.0	0.0	0.0	0.5170337233310393	0.0
38	0.0	0.0	0.0	0.756194081211287	0.0
39	0.0	0.0	0.0	1.0396593255333793	0.0
40	0.0	0.0	0.0	1.3665691672401927	0.0
41	0.0	0.0	0.0	1.7407949070887818	0.0
42	0.0	0.0	0.0	2.173520302821748	0.0
43	0.0	0.0	0.0	2.6720578114246387	0.0
44	0.0	0.0	0.0	3.276410874053682	0.0
45	0.0	0.0	0.0	3.959910529938059	0.0
46	0.0	0.0	0.0	4.659325533379215	0.0
47	0.0	0.0	0.0	5.406056434962148	0.0
48	0.0	0.0	0.0	6.104181004817619	0.0
49	0.0	0.0	0.0	6.820801789401239	0.0
50	0.0	0.0	0.0	7.472040605643496	0.0
51	0.0	0.0	0.0	8.088867859600827	0.0
52	0.0	0.0	0.0	8.715588437715072	0.0
53	0.0	0.0	0.0	9.333275980729525	0.0
54	0.0	0.0	0.0	10.022367515485204	0.0
55	0.0	0.0	0.0	10.768238128011012	0.0
56	0.0	0.0	0.0	11.539057123193393	0.0
57	0.0	0.0	0.0	12.284067446662078	0.0
58	0.0	0.0	0.0	13.050154852030282	0.0
59	0.0	0.0	0.0	13.762474191328286	0.0
60	0.0	0.0	0.0	14.434790089470061	0.0
61	0.0	0.0	0.0	15.100223675154853	0.0
62	0.0	0.0	0.0	15.76006538196834	0.0
63	0.0	0.0	0.0	16.381194081211287	0.0
64	0.0	0.0	0.0	16.988988300068822	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	335	0.0	56.41791	47
CGTCTTC	340	0.0	55.588234	48
TCGTATG	355	0.0	54.225353	40
CTCGTAT	345	0.0	53.768116	39
CGTATGC	365	0.0	53.698627	41
TATGCCG	375	0.0	53.2	43
AACCGAG	20	0.006945097	52.5	46
TACGGAG	20	0.006945097	52.5	52
CTACGGA	20	0.006945097	52.5	51
GCCGTCT	380	0.0	50.65789	46
TGCCGTC	395	0.0	50.506332	45
ATCTCGT	295	0.0	49.83051	37
GTATGCC	395	0.0	49.620255	42
ATGCCGT	405	0.0	49.25926	44
TCTCGTA	300	0.0	49.000004	38
CATCTCG	305	0.0	47.04918	36
GTCTTCT	395	0.0	46.96203	49
CTGCGTT	30	5.869674E-4	46.666668	23
CTTGAAA	425	0.0	44.47059	57
TCTATCG	40	4.583447E-5	43.75	29
>>END_MODULE