FastQCFastQC Report
Wed 25 May 2016
SRR1781123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355883
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGA200.006948114452.49999655
GATTCGC200.006948114452.49999665
ATCGATA200.006948114452.49999623
TCGCTTT400.002417197534.99999611
GAACCGT400.002417197534.99999669
ACCGCGT552.989334E-431.818188
TTAACGG450.00430450431.11110935
TTCGGTC500.007203785228.00000228
CTAGAGC755.577392E-528.03
GGTTCTA755.577392E-528.01
GGCCTTA657.948482E-426.9230771
CCGAAAT700.001224890625.00000247
TAGAGCC750.001830556523.3333344
GGTATAC750.001830556523.3333341
TATGACA901.9252341E-423.3333324
CCTTTAC1102.916146E-522.2727261
GACCCAA952.776182E-422.10526311
GTGCCGT952.776182E-422.10526370
TAATGTA800.002663565621.87499826
TTAGTAC1003.9262735E-421.0000023
Produced by FastQC (version 0.11.5)