##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781123_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355883
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431894751926897	34.0	33.0	34.0	23.0	34.0
2	31.53454927602611	34.0	33.0	34.0	24.0	34.0
3	31.511092128592825	34.0	33.0	34.0	23.0	34.0
4	31.664277304619777	34.0	34.0	34.0	23.0	34.0
5	31.63391339288474	34.0	34.0	34.0	23.0	34.0
6	34.41244453935703	37.0	34.0	38.0	26.0	38.0
7	34.34318863221902	37.0	34.0	38.0	24.0	38.0
8	34.29214938617467	37.0	34.0	38.0	24.0	38.0
9	34.53134597606517	37.0	34.0	38.0	26.0	38.0
10-11	34.429306822747925	38.0	34.0	38.0	25.5	38.0
12-13	34.38041575461598	38.0	34.0	38.0	25.0	38.0
14-15	34.27620032426387	37.0	34.0	38.0	24.0	38.0
16-17	34.23905187940981	37.0	34.0	38.0	24.5	38.0
18-19	34.28286543611243	37.0	34.0	38.0	24.5	38.0
20-21	34.2229932309214	37.0	34.0	38.0	24.5	38.0
22-23	34.2760022254505	37.0	34.0	38.0	24.5	38.0
24-25	34.26111531036886	37.0	34.0	38.0	24.0	38.0
26-27	34.18016314350503	37.0	34.0	38.0	24.0	38.0
28-29	34.26950289842448	37.0	34.0	38.0	24.0	38.0
30-31	34.34555036346215	37.0	34.0	38.0	25.0	38.0
32-33	34.30016466085765	37.0	34.0	38.0	24.5	38.0
34-35	34.32731122307051	37.0	34.0	38.0	24.5	38.0
36-37	34.17705819047271	37.0	34.0	38.0	24.0	38.0
38-39	34.201165832591045	37.0	34.0	38.0	24.0	38.0
40-41	34.18119438129947	37.0	34.0	38.0	24.0	38.0
42-43	34.26767926537654	37.0	34.0	38.0	24.0	38.0
44-45	34.282747419798085	37.0	34.0	38.0	24.5	38.0
46-47	34.28384328557419	37.0	34.0	38.0	24.5	38.0
48-49	34.23946072164166	37.0	34.0	38.0	24.0	38.0
50-51	34.11810904145464	37.0	34.0	38.0	24.0	38.0
52-53	34.19802575565565	37.0	34.0	38.0	24.0	38.0
54-55	34.15943161095079	37.0	34.0	38.0	24.0	38.0
56-57	34.12178300171686	37.0	34.0	38.0	24.0	38.0
58-59	34.118295900619025	37.0	34.0	38.0	24.0	38.0
60-61	34.10371105110387	37.0	34.0	38.0	24.0	38.0
62-63	34.07720936375157	37.0	34.0	38.0	24.0	38.0
64-65	34.10140551810567	37.0	34.0	38.0	24.0	38.0
66-67	33.994132903229435	37.0	34.0	38.0	24.0	38.0
68-69	33.771905373395185	37.0	34.0	38.0	23.5	38.0
70-71	33.922856669186224	37.0	34.0	38.0	23.5	38.0
72-73	33.88525020863598	37.0	34.0	38.0	23.0	38.0
74-75	33.86049488174484	37.0	34.0	38.0	23.5	38.0
76	32.52497871491473	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	26.0
14	112.0
15	326.0
16	664.0
17	1174.0
18	1666.0
19	2022.0
20	2247.0
21	2716.0
22	3024.0
23	3484.0
24	4355.0
25	5111.0
26	6048.0
27	7202.0
28	8284.0
29	10223.0
30	11985.0
31	14218.0
32	17249.0
33	21277.0
34	27058.0
35	35505.0
36	53710.0
37	116191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.831683446525965	18.529966309152165	9.659354338364013	24.978995905957856
2	23.429048310821255	20.877647991053237	31.10516658564753	24.588137112477977
3	19.438691929651036	22.03392688046352	29.994970257078872	28.532410932806567
4	14.086090091406444	16.69087874385683	36.77107363937024	32.45195752536648
5	13.84443763821256	33.17494794637563	36.236909321321896	16.74370509408991
6	29.92275551234535	36.63788379888896	18.97308947041584	14.466271218349853
7	29.081186794536407	29.715102997333393	22.12047217765389	19.083238030476306
8	25.935209043421576	34.42732583461419	20.19512030639283	19.44234481557141
9	24.68957494457448	17.32844783257419	21.75293565581947	36.229041567031864
10-11	25.351730765448195	24.731442637046445	27.385826240646505	22.531000356858854
12-13	25.363813388107893	23.148478573014163	27.058330968323858	24.429377070554086
14-15	23.189924778649164	24.069146320560407	24.414063048810984	28.326865851979445
16-17	23.24724698847655	27.527305322254787	25.42141658915992	23.804031100108745
18-19	22.917784777581396	27.025454995040505	26.779728169089278	23.277032058288817
20-21	23.06151178898683	26.791810791748972	26.65370360483642	23.492973814427774
22-23	23.208189208251024	26.35318349007961	27.012248407482236	23.426378894187135
24-25	23.05476799959537	26.673372990561507	26.757389366730077	23.514469643113046
26-27	22.44979389293672	26.855455304130853	27.073363998842314	23.621386804090108
28-29	22.883222856950177	26.696273775369995	27.618767965876422	22.801735401803402
30-31	23.082867122059778	26.643728416361554	27.040066538721995	23.23333792285667
32-33	22.52355408940579	27.110033353658363	26.91713287794022	23.449279678995623
34-35	23.195123116305076	26.240084522160373	27.30180986447793	23.262982497056615
36-37	23.175734721804638	26.566736820809083	26.842810699021868	23.414717758364407
38-39	22.619934079458698	26.90940561926251	27.431065827814198	23.039594473464593
40-41	22.83714029610855	26.183745781619237	27.74310658278142	23.23600733949079
42-43	22.55151271625787	26.27029107880961	27.410131981578218	23.768064223354305
44-45	22.27122396967543	26.240927495834303	27.595726685455613	23.892121849034655
46-47	22.286397495806206	25.858357943481426	27.988973904344967	23.8662706563674
48-49	21.771481076645976	25.96190320976276	28.59647693202541	23.670138781565853
50-51	21.842712352093244	25.84430838224922	28.327849321265695	23.985129944391836
52-53	21.581109521949628	25.75818457189582	28.843327722875216	23.817378183279338
54-55	21.172688776929498	26.263125802581182	28.617410778261394	23.946774642227922
56-57	20.924995012405763	26.089332730138835	28.62879092285948	24.35688133459592
58-59	21.048771646861468	26.006861805705807	28.512741547081482	24.43162500035124
60-61	20.916143788829476	25.93211813995049	28.5013614024834	24.650376668736634
62-63	20.63922693694276	25.77181264629106	28.685270159012934	24.90369025775325
64-65	21.15821772886033	25.92115948218937	28.14857691994279	24.77204586900751
66-67	21.119440939859448	25.730647431880705	27.967056588822732	25.18285503943712
68-69	21.117895488123906	25.592118758131182	27.690701719385302	25.59928403435961
70-71	21.467308075968788	25.584672490678116	27.689577754486727	25.258441678866365
72-73	21.49231629496211	25.134524548798343	27.399735306266386	25.973423849973166
74-75	21.707555573039453	25.300590362562975	27.284809895386967	25.7070441690106
76	21.731018340297233	25.02002062475589	27.195173694725515	26.053787340221362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	3.0
11	3.5
12	1.0
13	5.0
14	14.5
15	40.0
16	88.5
17	117.0
18	152.5
19	218.0
20	345.5
21	443.0
22	551.5
23	797.5
24	1197.0
25	1459.0
26	1740.5
27	2314.5
28	2975.0
29	3343.0
30	3809.0
31	4831.0
32	6179.0
33	6971.0
34	7885.0
35	9659.0
36	11546.5
37	12574.0
38	13551.5
39	15739.0
40	18077.5
41	19875.5
42	20545.0
43	20800.5
44	21314.5
45	21345.0
46	21117.0
47	20607.5
48	19259.5
49	17568.0
50	16715.0
51	15594.0
52	13246.0
53	11352.5
54	10686.0
55	9898.0
56	8358.0
57	7009.0
58	6412.0
59	5777.5
60	4812.5
61	4168.5
62	3855.0
63	3617.5
64	3265.0
65	3035.5
66	2777.0
67	2633.0
68	2600.5
69	2496.5
70	2306.0
71	2187.0
72	2145.0
73	1977.0
74	1790.0
75	1729.0
76	1658.5
77	1428.0
78	1141.5
79	1015.0
80	881.5
81	656.0
82	481.5
83	399.0
84	334.5
85	221.5
86	138.5
87	104.0
88	82.5
89	51.5
90	30.5
91	18.0
92	17.0
93	11.5
94	4.5
95	4.5
96	6.0
97	5.0
98	2.5
99	4.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	355883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.8970059755935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59576288885496	37.872014973589366
2	15.000807203613556	15.869955765765445
3	6.0323688573640775	9.57282754484915
4	2.427919221491848	5.1371863027005045
5	1.1047450944734274	2.9218853931934246
6	0.6418623261628963	2.037155718152824
7	0.4641576965856156	1.7186786714934916
8	0.3460346576180319	1.4643357881426786
9	0.29742303645988266	1.4159509323207817
>10	2.0151506470990297	19.446889576017405
>50	0.07111483177747356	2.390964495613359
>100	0.002653538499159369	0.1521548381616066
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.8099122464405436E-4	0.0
2	0.0	0.0	0.0	2.8099122464405436E-4	0.0
3	0.0	0.0	0.0	2.8099122464405436E-4	0.0
4	0.0	0.0	0.0	2.8099122464405436E-4	0.0
5	0.0	0.0	0.0	2.8099122464405436E-4	0.0
6	0.0	0.0	0.0	2.8099122464405436E-4	0.0
7	0.0	0.0	0.0	2.8099122464405436E-4	0.0
8	0.0	0.0	0.0	2.8099122464405436E-4	0.0
9	0.0	0.0	0.0	2.8099122464405436E-4	0.0
10	0.0	0.0	0.0	8.429736739321631E-4	0.0
11	0.0	0.0	0.0	0.0011239648985762174	0.0
12	0.0	0.0	0.0	0.0011239648985762174	0.0
13	0.0	0.0	0.0	0.0014049561232202718	0.0
14	0.0	0.0	0.0	0.0014049561232202718	0.0
15	0.0	0.0	0.0	0.0014049561232202718	0.0
16	0.0	0.0	0.0	0.0016859473478643262	0.0
17	0.0	0.0	0.0	0.0019669385725083807	0.0
18	0.0	0.0	0.0	0.0025289210217964895	0.0
19	0.0	0.0	0.0	0.0025289210217964895	8.429736739321631E-4
20	0.0	0.0	0.0	0.0025289210217964895	8.429736739321631E-4
21	0.0	0.0	0.0	0.0025289210217964895	8.429736739321631E-4
22	0.0	0.0	0.0	0.0036528859203727067	8.429736739321631E-4
23	0.0	0.0	0.0	0.00449585959430487	8.429736739321631E-4
24	0.0	0.0	0.0	0.006181806942169196	8.429736739321631E-4
25	0.0	0.0	0.0	0.006181806942169196	8.429736739321631E-4
26	0.0	0.0	0.0	0.006743789391457305	8.429736739321631E-4
27	0.0	0.0	0.0	0.01095865776111812	8.429736739321631E-4
28	0.0	0.0	0.0	0.024727227768676783	8.429736739321631E-4
29	0.0	0.0	0.0	0.037652824102303285	8.429736739321631E-4
30	0.0	0.0	0.0	0.04917346431270951	8.429736739321631E-4
31	0.0	0.0	0.0	0.06518996411742062	8.429736739321631E-4
32	0.0	0.0	0.0	0.08289241126999604	8.429736739321631E-4
33	0.0	0.0	0.0	0.11127252495904554	8.429736739321631E-4
34	0.0	0.0	0.0	0.1545451735542299	8.429736739321631E-4
35	0.0	0.0	0.0	0.22170207624415889	8.429736739321631E-4
36	0.0	0.0	0.0	0.3220159434420863	8.429736739321631E-4
37	0.0	0.0	0.0	0.49679248517068814	8.429736739321631E-4
38	0.0	0.0	0.0	0.71315572814661	8.429736739321631E-4
39	0.0	0.0	0.0	0.9829073038049022	8.429736739321631E-4
40	0.0	0.0	0.0	1.2863778264204808	8.429736739321631E-4
41	0.0	0.0	0.0	1.6272201819137189	8.429736739321631E-4
42	0.0	0.0	0.0	2.0177979841689546	8.429736739321631E-4
43	0.0	0.0	0.0	2.4794665662591355	8.429736739321631E-4
44	0.0	0.0	0.0	3.011101963285687	8.429736739321631E-4
45	0.0	0.0	0.0	3.6253487803575895	8.429736739321631E-4
46	0.0	0.0	0.0	4.282587254800033	8.429736739321631E-4
47	0.0	0.0	0.0	4.939263746793188	8.429736739321631E-4
48	0.0	0.0	0.0	5.5498576779447175	8.429736739321631E-4
49	0.0	0.0	0.0	6.154831784603367	8.429736739321631E-4
50	0.0	0.0	0.0	6.746880294928389	8.429736739321631E-4
51	0.0	0.0	0.0	7.323755279122633	8.429736739321631E-4
52	0.0	0.0	0.0	7.924514517411621	8.429736739321631E-4
53	0.0	0.0	0.0	8.516282036511999	8.429736739321631E-4
54	0.0	0.0	0.0	9.116760283576344	8.429736739321631E-4
55	0.0	0.0	0.0	9.799006977012107	8.429736739321631E-4
56	0.0	0.0	0.0	10.44865868838916	8.429736739321631E-4
57	0.0	0.0	0.0	11.078360022816488	8.429736739321631E-4
58	0.0	0.0	0.0	11.726044795621032	8.429736739321631E-4
59	0.0	0.0	0.0	12.28100246429304	8.429736739321631E-4
60	0.0	0.0	0.0	12.862092316856945	8.429736739321631E-4
61	0.0	0.0	0.0	13.41002520491285	8.429736739321631E-4
62	0.0	0.0	0.0	13.959082057867333	8.429736739321631E-4
63	0.0	0.0	0.0	14.522469463278663	8.429736739321631E-4
64	0.0	0.0	0.0	15.1184518507487	8.429736739321631E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGA	20	0.0069481144	52.499996	55
GATTCGC	20	0.0069481144	52.499996	65
ATCGATA	20	0.0069481144	52.499996	23
TCGCTTT	40	0.0024171975	34.999996	11
GAACCGT	40	0.0024171975	34.999996	69
ACCGCGT	55	2.989334E-4	31.81818	8
TTAACGG	45	0.004304504	31.111109	35
TTCGGTC	50	0.0072037852	28.000002	28
CTAGAGC	75	5.577392E-5	28.0	3
GGTTCTA	75	5.577392E-5	28.0	1
GGCCTTA	65	7.948482E-4	26.923077	1
CCGAAAT	70	0.0012248906	25.000002	47
TAGAGCC	75	0.0018305565	23.333334	4
GGTATAC	75	0.0018305565	23.333334	1
TATGACA	90	1.9252341E-4	23.333332	4
CCTTTAC	110	2.916146E-5	22.272726	1
GACCCAA	95	2.776182E-4	22.105263	11
GTGCCGT	95	2.776182E-4	22.105263	70
TAATGTA	80	0.0026635656	21.874998	26
TTAGTAC	100	3.9262735E-4	21.000002	3
>>END_MODULE