FastQCFastQC Report
Wed 25 May 2016
SRR1781123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321717
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT24090.7487947481793005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT150.002223444470.014
CGTATGC3900.052.9487241
TCGTATG4000.052.50000440
CGTGCGA200.006947511352.49999610
CTCGTAT3800.051.57894539
TATGCCG3950.051.39240643
TCTCGTA3350.051.19402738
CGTCTTC3750.050.39999848
CCGTCTT3900.050.2564147
TACGCGC506.182927E-849.00000470
ATCTCGT3550.048.3098637
GTATGCC4400.047.72727242
ATGCCGT4200.047.544
TGCCGTC4200.047.545
GCCGTCT4300.047.209346
GTACGCG551.3086719E-744.54545269
CTTGAAA4300.043.95348757
GTGTACG602.5920417E-740.83333267
TATCTCG4150.040.4819336
CTGCTTG4600.040.32608854
Produced by FastQC (version 0.11.5)