##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781123_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321717
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.27408871772396	34.0	34.0	34.0	32.0	34.0
2	33.27614331850664	34.0	34.0	34.0	33.0	34.0
3	33.32575835283805	34.0	34.0	34.0	33.0	34.0
4	33.387365293099215	34.0	34.0	34.0	33.0	34.0
5	33.3709844366322	34.0	34.0	34.0	33.0	34.0
6	36.790483561639576	38.0	37.0	38.0	34.0	38.0
7	36.68917091729688	38.0	37.0	38.0	34.0	38.0
8	36.47471535542107	38.0	37.0	38.0	34.0	38.0
9	36.442040675500515	38.0	37.0	38.0	34.0	38.0
10-11	36.35644992337987	38.0	37.0	38.0	34.0	38.0
12-13	36.28595784493825	38.0	37.0	38.0	34.0	38.0
14-15	36.297837851279226	38.0	37.0	38.0	34.0	38.0
16-17	36.25764258649683	38.0	37.0	38.0	34.0	38.0
18-19	36.25044526711365	38.0	37.0	38.0	34.0	38.0
20-21	36.195813401219084	38.0	37.0	38.0	33.5	38.0
22-23	36.23428199318034	38.0	37.0	38.0	34.0	38.0
24-25	36.22807622848653	38.0	37.0	38.0	34.0	38.0
26-27	36.01430449743097	38.0	37.0	38.0	32.0	38.0
28-29	36.00014142864691	38.0	37.0	38.0	32.0	38.0
30-31	36.027640752586905	38.0	37.0	38.0	32.0	38.0
32-33	35.98031188902047	38.0	37.0	38.0	32.0	38.0
34-35	35.98036783881486	38.0	37.0	38.0	32.0	38.0
36-37	35.9470217613617	38.0	37.0	38.0	32.0	38.0
38-39	35.92135013070494	38.0	37.0	38.0	32.0	38.0
40-41	35.9356996988036	38.0	37.0	38.0	32.0	38.0
42-43	35.92657211151416	38.0	37.0	38.0	32.0	38.0
44-45	35.89309703870172	38.0	36.5	38.0	32.0	38.0
46-47	35.90512624449438	38.0	37.0	38.0	32.0	38.0
48-49	35.889816204925445	38.0	37.0	38.0	32.0	38.0
50-51	35.80649141947737	38.0	36.0	38.0	31.0	38.0
52-53	35.84466472085715	38.0	36.0	38.0	31.5	38.0
54-55	35.855717913570004	38.0	36.0	38.0	31.5	38.0
56-57	35.80082184031307	38.0	36.0	38.0	31.0	38.0
58-59	35.80897963116652	38.0	36.0	38.0	31.0	38.0
60-61	35.81127201857533	38.0	36.0	38.0	31.0	38.0
62-63	35.78684060836076	38.0	36.0	38.0	31.0	38.0
64-65	35.73387946549296	38.0	36.0	38.0	31.0	38.0
66-67	35.72044996067973	38.0	36.0	38.0	31.0	38.0
68-69	35.67374586981727	38.0	36.0	38.0	31.0	38.0
70-71	35.61572748720148	38.0	36.0	38.0	31.0	38.0
72-73	35.56071018939005	38.0	36.0	38.0	31.0	38.0
74-75	35.56881669293199	38.0	36.0	38.0	31.0	38.0
76	34.71164408470799	37.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	14.0
22	42.0
23	94.0
24	212.0
25	434.0
26	903.0
27	1543.0
28	2672.0
29	4283.0
30	6624.0
31	9904.0
32	14111.0
33	19561.0
34	26586.0
35	36870.0
36	56356.0
37	141503.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.33922671167517	18.448201369526636	10.113857831572469	26.098714087225726
2	23.31863097069785	21.096802469250925	30.949250428171343	24.635316131879883
3	20.367279316915177	22.44581417829956	29.253660826129796	27.933245678655467
4	14.269062561195087	17.362153694085176	36.41709950049267	31.951684244227067
5	13.688428028360331	34.08647973218699	36.017058470643455	16.208033768809234
6	30.191130714261295	37.769219531451554	18.04380868900307	13.995841065284084
7	28.95588358712782	29.907651756046462	21.402039680837508	19.73442497598821
8	25.66727900608299	35.57101427652253	19.168088723940606	19.593617993453876
9	24.5858938135069	17.202696780089333	20.967185445593486	37.24422396081028
10-11	25.035978826111148	25.39250334921686	27.175281380840925	22.396236443831068
12-13	25.114153122153944	23.148605762207158	26.979457100495157	24.757784015143745
14-15	22.88315507107178	23.98909600673884	23.890562202183908	29.23718672000547
16-17	23.719604497120137	27.72421103019113	24.938377518129286	23.61780695455944
18-19	23.416698526966247	27.004479091872668	26.105396979332767	23.473425401828315
20-21	22.752605550841267	26.99888411243422	26.035925984638673	24.212584352085837
22-23	23.046031139168896	26.931744359172814	26.307437903499036	23.71478659815925
24-25	23.33961214359204	27.027480674008526	26.36929350951302	23.263613672886418
26-27	22.74359141730154	27.407006779250086	26.5279733430313	23.321428460417074
28-29	22.583046590637114	26.627905892445845	27.169220153115937	23.619827363801104
30-31	22.86155223379554	27.58589070518499	26.576618580926713	22.975938480092754
32-33	22.833888168794314	27.499168523889004	26.54957618030754	23.117367127009143
34-35	22.932732805540272	26.93205519136384	27.217243726629302	22.91796827646658
36-37	22.873363857054493	26.81300646220125	27.054678490723212	23.258951190021044
38-39	22.25791611882493	26.88045704765368	27.482849833860197	23.378776999661195
40-41	22.53673259417438	26.144251003210893	27.924697793402274	23.394318609212444
42-43	22.932888221635785	26.00779567135091	27.54734751349789	23.51196859351542
44-45	21.904966165916008	26.777571592424398	27.730427674011633	23.58703456764796
46-47	22.5642412430801	25.83699338238265	27.596614415775356	24.00215095876189
48-49	22.042509410444584	25.9045993839306	28.144766984647994	23.90812422097682
50-51	21.559787017782707	26.008883584019493	28.43881423735768	23.992515160840117
52-53	21.234190297683988	25.632776632879207	29.299197742114963	23.83383532732184
54-55	20.60413344647625	26.33106115001694	28.945936957015018	24.118868446491792
56-57	21.011012784528017	26.114255696776983	28.169478143834485	24.705253374860515
58-59	20.558285698300057	25.854555401175567	28.986655974039294	24.600502926485078
60-61	20.93097349533907	26.37364516018737	28.164349412682576	24.531031931790984
62-63	20.00609231094409	26.710121006971967	28.06472769545905	25.219058986624894
64-65	20.148453454433554	26.932987687936915	27.453165359617305	25.46539349801223
66-67	20.32236406531206	26.91060777018311	26.84206927206209	25.924958892442735
68-69	20.23533105182505	27.163003509295436	26.830723897089676	25.77094154178983
70-71	20.525026653860383	27.0694430197969	26.28925422032407	26.116276106018642
72-73	20.523783325096282	26.523000027974895	26.262056403609385	26.69116024331944
74-75	20.572739395182722	26.936096009847166	25.92091807395941	26.570246521010706
76	20.85124503834115	27.14031275935061	25.566258543999854	26.442183658308387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.5
11	1.5
12	1.0
13	4.5
14	13.5
15	33.0
16	67.0
17	87.0
18	122.0
19	218.5
20	325.5
21	371.0
22	505.0
23	812.5
24	1178.0
25	1370.0
26	1655.5
27	2190.0
28	2786.5
29	3134.0
30	3552.5
31	4453.5
32	5652.5
33	6369.0
34	7038.5
35	8503.5
36	10077.5
37	10856.0
38	11956.5
39	14485.5
40	16947.0
41	18508.0
42	19036.0
43	19118.0
44	19148.5
45	19065.0
46	19033.0
47	18689.0
48	17412.5
49	15807.0
50	15134.0
51	13979.5
52	11762.5
53	10160.5
54	9621.0
55	8913.5
56	7544.5
57	6280.0
58	5677.0
59	5067.0
60	4167.5
61	3591.0
62	3304.0
63	3102.5
64	2836.5
65	2694.5
66	2451.5
67	2286.0
68	2292.5
69	2207.5
70	2087.5
71	2059.0
72	1995.5
73	1854.0
74	1685.5
75	1595.0
76	1496.0
77	1297.5
78	1082.5
79	967.0
80	870.5
81	652.5
82	461.0
83	391.0
84	322.5
85	215.5
86	150.0
87	123.0
88	87.0
89	35.5
90	21.0
91	13.5
92	5.0
93	3.0
94	2.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	321717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.4875540708617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.30722049259617	27.887155070912083
2	20.738810940160693	18.867155680118753
3	8.287949164037062	11.309956072070666
4	3.2617825182403437	5.934820346633963
5	1.4535492339181917	3.3059199686256675
6	0.8059924499373425	2.1997575088338697
7	0.601320636824452	1.9146823487034077
8	0.45401907007741454	1.6521773599478995
9	0.39466349543014545	1.6157049379356618
>10	2.5986533187680445	21.553520825015152
>50	0.0878067931521817	2.506307947690018
>100	0.007545896286515615	0.5011367734051505
>500	0.0	0.0
>1k	6.859905715014196E-4	0.7517051601077258
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	2409	0.7487947481793005	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.216643820500627E-4	0.0
2	0.0	0.0	0.0	6.216643820500627E-4	0.0
3	0.0	0.0	0.0	6.216643820500627E-4	0.0
4	0.0	0.0	0.0	6.216643820500627E-4	0.0
5	0.0	0.0	0.0	6.216643820500627E-4	0.0
6	0.0	0.0	0.0	6.216643820500627E-4	0.0
7	0.0	0.0	0.0	6.216643820500627E-4	0.0
8	0.0	0.0	0.0	6.216643820500627E-4	0.0
9	0.0	0.0	0.0	6.216643820500627E-4	0.0
10	0.0	0.0	0.0	0.0012433287641001253	0.0
11	0.0	0.0	0.0	0.0015541609551251567	0.0
12	0.0	0.0	0.0	0.0015541609551251567	0.0
13	0.0	0.0	0.0	0.001864993146150188	0.0
14	0.0	0.0	0.0	0.001864993146150188	0.0
15	0.0	0.0	0.0	0.0021758253371752193	0.0
16	0.0	0.0	0.0	0.0024866575282002507	0.0
17	0.0	0.0	0.0	0.002797489719225282	0.0
18	0.0	0.0	0.0	0.003729986292300376	0.0
19	0.0	0.0	0.0	0.004040818483325407	0.0
20	0.0	0.0	0.0	0.004040818483325407	0.0
21	0.0	0.0	0.0	0.004040818483325407	0.0
22	0.0	0.0	0.0	0.004040818483325407	0.0
23	0.0	0.0	0.0	0.005284147247425533	0.0
24	0.0	0.0	0.0	0.006838308202550689	0.0
25	0.0	0.0	0.0	0.006838308202550689	0.0
26	0.0	0.0	0.0	0.007459972584600752	0.0
27	0.0	0.0	0.0	0.01181162325895119	0.0
28	0.0	0.0	0.0	0.026731568428152695	0.0
29	0.0	0.0	0.0	0.0407190170242791	0.0
30	0.0	0.0	0.0	0.05190897590118023	0.0
31	0.0	0.0	0.0	0.06838308202550689	0.0
32	0.0	0.0	0.0	0.08703301348700877	0.0
33	0.0	0.0	0.0	0.12091372230873718	0.0
34	0.0	0.0	0.0	0.16847104753556696	0.0
35	0.0	0.0	0.0	0.24182744461747435	0.0
36	0.0	0.0	0.0	0.35092954366726037	0.0
37	0.0	0.0	0.0	0.5402263480015044	0.0
38	0.0	0.0	0.0	0.7755263166074531	0.0
39	0.0	0.0	0.0	1.0630460933056072	0.0
40	0.0	0.0	0.0	1.3844465788254894	0.0
41	0.0	0.0	0.0	1.7474985779427261	0.0
42	0.0	0.0	0.0	2.162770385152168	0.0
43	0.0	0.0	0.0	2.6588585620281178	0.0
44	0.0	0.0	0.0	3.2245731496936747	0.0
45	0.0	0.0	0.0	3.8794965761834157	0.0
46	0.0	0.0	0.0	4.577936509416661	0.0
47	0.0	0.0	0.0	5.268294805683256	0.0
48	0.0	0.0	0.0	5.9086091191948205	0.0
49	0.0	0.0	0.0	6.54674760736921	0.0
50	0.0	0.0	0.0	7.171520311329522	0.0
51	0.0	0.0	0.0	7.771737272198858	0.0
52	0.0	0.0	0.0	8.408321599418121	0.0
53	0.0	0.0	0.0	9.022526008883585	0.0
54	0.0	0.0	0.0	9.657867007338748	0.0
55	0.0	0.0	0.0	10.36594273849377	0.0
56	0.0	0.0	0.0	11.051638551894989	0.0
57	0.0	0.0	0.0	11.736401868723133	0.0
58	0.0	0.0	0.0	12.40469107942695	0.0
59	0.0	0.0	0.0	13.022625475184713	0.0
60	0.0	0.0	0.0	13.622842436054048	0.0
61	0.0	0.0	0.0	14.205341962034955	0.0
62	0.0	0.0	0.0	14.785976494869715	0.0
63	0.0	0.0	0.0	15.369719349614723	0.0
64	0.0	0.0	0.0	15.99169456385581	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGAAT	15	0.0022234444	70.0	14
CGTATGC	390	0.0	52.94872	41
TCGTATG	400	0.0	52.500004	40
CGTGCGA	20	0.0069475113	52.499996	10
CTCGTAT	380	0.0	51.578945	39
TATGCCG	395	0.0	51.392406	43
TCTCGTA	335	0.0	51.194027	38
CGTCTTC	375	0.0	50.399998	48
CCGTCTT	390	0.0	50.25641	47
TACGCGC	50	6.182927E-8	49.000004	70
ATCTCGT	355	0.0	48.30986	37
GTATGCC	440	0.0	47.727272	42
ATGCCGT	420	0.0	47.5	44
TGCCGTC	420	0.0	47.5	45
GCCGTCT	430	0.0	47.2093	46
GTACGCG	55	1.3086719E-7	44.545452	69
CTTGAAA	430	0.0	43.953487	57
GTGTACG	60	2.5920417E-7	40.833332	67
TATCTCG	415	0.0	40.48193	36
CTGCTTG	460	0.0	40.326088	54
>>END_MODULE