FastQCFastQC Report
Wed 25 May 2016
SRR1781122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442825
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7910317.863264269180828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG200.006949760552.4989855
GCGATCC200.006949760552.49898532
CGACGAG305.8760267E-446.66576422
TGGTCGC1200.043.74915349
TCGCCGT1050.043.33249752
GTCGCCG1150.042.60787251
GCCGTAT953.6379788E-1240.5255354
GTCTCGG350.001255052339.9992342
TCGGTGG1750.039.9992345
ATACACG350.001255052339.999234
GTCGGTG459.193784E-538.88813844
GCGACAA556.690776E-638.1810841
GTGGTCG1500.037.3326148
GCGCTGC1051.4551915E-1136.6659615
CTGGCGC1450.036.20619612
CGGCGAG501.7089833E-434.99932522
CGTATCA1003.255991E-1034.99932556
TGGCGCT1500.034.99932513
CACGCAC400.002418062234.9993257
CCTACCG501.7089833E-434.99932549
Produced by FastQC (version 0.11.5)