FastQCFastQC Report
Wed 25 May 2016
SRR1781122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442825
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4801310.84243211200813No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG90332.039857731609552No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC33760.7623779145260543No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC18910.42703099418506185No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT17720.40015807598938635Illumina PCR Primer Index 3 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT16030.36199401569468753No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC11650.26308361090724325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5480.12375091740529556No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG4550.10274939310111217No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4480.10116863320724892No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGA150.002224198369.9984155
AACGACT150.002224198369.9984111
AGGGGGG58750.062.1347770
TCCGGCG200.006949849452.49881422
GCCGTCA1500.048.99889446
CGTCTTC81400.048.54313348
GCCGTCT81450.048.51333246
CCGTCTT81200.048.49028447
CGTATGC84300.048.45086341
TGCCGTC82450.048.4343245
ATGCCGT83150.048.405444
TATGCCG83300.048.40226743
CTCGTAT84550.048.34939
GTATGCC83750.048.26756342
TCGTATG85900.048.24105540
TCTCGTA84700.048.1394138
CGTCTTA800.048.12391348
CATCTCG84050.047.97036436
ATCTCGT84950.047.87413837
CTGCTTG83000.047.7760354
Produced by FastQC (version 0.11.5)