FastQCFastQC Report
Wed 25 May 2016
SRR1781121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152832
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6769344.29242567001675No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAACAGTGTAGATCTCGGTG2450.1603067420435511No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGC207.894042E-570.022581
CCTTCAA150.002221245669.9996715
GTACAGC150.002221245669.999672
GCGCGTC252.8292925E-669.999676
CAGCAAT150.002221245669.999675
TGTGCGC150.002221245669.999673
TACAGCA150.002221245669.999673
CGCGTCT401.0590156E-861.2497147
TGCCGGC308.345745E-658.33305719
CGTATCA553.45608E-1157.2724656
TCGCCGT509.731593E-1055.99973352
GTCTGGC509.731593E-1055.99973310
TCTTAGG252.3845144E-455.9997332
TGCGCGT252.3845144E-455.9997335
GCAATCA252.3845144E-455.9997337
TCGGTGG1150.054.78235245
GCGCTGG452.6830094E-854.44418715
TCTGGCG653.6379788E-1253.845911
CGGCGGT200.00694069452.49975222
ACTGAGT200.00694069452.49975250
Produced by FastQC (version 0.11.5)