FastQCFastQC Report
Wed 25 May 2016
SRR1781121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152832
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4089026.75486809045226No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG71714.692080192629815No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC23581.5428706030150754No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC16241.062604690117253No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT13570.8879030569514237No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT13420.8780883584589614RNA PCR Primer, Index 34 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC9600.628140703517588No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4320.2826633165829146No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT3950.2584537269681742No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG3870.25321922110552764No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3720.24340452261306533No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC3710.2427502093802345No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT3620.23686139028475714No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT3320.2172319932998325RNA PCR Primer, Index 34 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3310.21657768006700168RNA PCR Primer, Index 34 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT3280.2146147403685092No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT3140.20545435510887772No Hit
CTTATACACATCTCCGAGCCAACGAGACCTCATGGGATCTCGTATGCCGT3020.1976025963149079No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT2660.17404731993299832No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTAAGCCGT2300.15049204355108878No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2270.14852910385259632No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCAGT2210.14460322445561138No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2130.13936871859296482No Hit
TCTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT2050.13413421273031828No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGATCTCGTATGCCGTC2010.13151695979899497No Hit
CTTATACACATCTCCGAGACCACGAGACCTCATGGGATCTCGTATGCCGT1570.10272717755443886No Hit
CTTATACACATCTCAGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1530.10010992462311558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAATAC301.0164695E-770.018661
ACTCCGA401.3278623E-1070.0186611
GTTGCAG150.002218856770.0186610
GAAATCG207.90905E-569.9957536
GGCGTTG150.002221739969.9957450
GACGTCA150.002221739969.9957446
TGACGAC301.0187796E-769.9957445
ATTCCGT150.002221739969.9957445
TGCGATC150.002221739969.9957433
TGATTGC150.002221739969.9957439
TGCTGTA150.002221739969.9957443
ATCTTGA150.002221739969.9957455
TTGAAGG150.002221739969.9957431
GCGATCT150.002221739969.9957434
GATTGCT150.002221739969.9957440
CCGAATT301.0187796E-769.9957447
AACTCGT301.0187796E-769.9957437
AACTCGA150.002221739969.9957437
GCAGCAA150.002221739969.9957413
CGACTTA150.002221739969.9957448
Produced by FastQC (version 0.11.5)