FastQCFastQC Report
Wed 25 May 2016
SRR1781120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274419
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7434627.09214740961814No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAACAGTGTAGATCTCGGTG2840.10349137632598326No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT150.0022232169.9990847
TCGCCGT352.967845E-759.99921852
GCCGTAT352.967845E-759.99921854
TCTCGGG308.363821E-658.33257343
CGACGAG252.387973E-455.9992722
GGTCGGA200.006946784452.49931329
TAAGCGT200.006946784452.4993135
GACGAGT305.8719167E-446.66605823
CGTATCA503.471485E-641.99945456
TGCGCGT602.590441E-740.8327985
CGCCACA350.001254179539.99947710
CGCCGTA556.6811863E-638.1813253
GTCGCCG753.525747E-837.33284851
CGCGTCT753.525747E-837.3328487
GCGCGTG400.002416390234.9995426
GCACTTA400.002416390234.99954261
TGGTCGC904.483809E-934.99954249
CTGGCGC1253.6379788E-1233.59956412
TCTGGCG1253.6379788E-1233.59956411
ATCTCGG751.4865109E-632.6662442
Produced by FastQC (version 0.11.5)