FastQCFastQC Report
Wed 25 May 2016
SRR1781120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274419
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4404516.050273486894127No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG80022.9159788498609793No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC24360.8876936363735747No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC17120.6238635079932512No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT17080.6224058829745754No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT16500.601270320203776TruSeq Adapter, Index 10 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC10540.3840841924210787No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG5100.1858471898811671No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4850.17673703351444325No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC4720.17199975220374683No Hit
CTTATACACATCTCCGAGCCAACGAGACACCGGCTAATCTCGTATGCCGT4130.15049978317827847No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC4030.14685572063158892No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3820.13920318928354086No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT3560.12972862666214802No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT3450.12572015786078952No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT3360.12244050156876893No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG2960.10786425138201072No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCGT150.002223507769.9967369
CGACTTA207.919718E-569.9967348
GCCGTAA150.002223507769.9967346
TGCCGTA900.066.1080145
AGGGGGG57800.063.09392570
GCCGTAT900.062.21930746
CTTAAGC308.365494E-658.33060551
CTCGAAA308.365494E-658.33060539
GAACACG509.786163E-1055.9973818
ATTATAC5100.054.9294131
TAAGCCG1150.054.78004543
ATCTCGA1300.053.84362837
CGGATAA653.6379788E-1253.84362831
CTTTATA3350.053.3102262
AACGTAT200.00694771352.49754339
GGTACGT200.00694771352.49754312
GTATTCT1000.052.49754349
CGTCTTA800.052.49754348
ATATCCG1000.052.49754310
GCCGAAA200.00694771352.49754346