FastQCFastQC Report
Wed 25 May 2016
SRR1781119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336962
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7244721.50005045079267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTCG150.002223629769.9992523
GGTCGGT252.3887292E-455.9994143
TCGTTGT200.00694808552.49944734
TTCGTCA200.00694808552.49944732
TGCGGGC407.4689706E-752.49944719
CGATAAG200.00694808552.49944725
CGTATCA701.9215804E-839.99957756
GGTGCGC350.001254571639.9995773
GACGATT350.001254571639.99957716
GTACGTA350.001254571639.99957753
TACGTAG350.001254571639.99957754
TGTACGT459.1886315E-538.88847752
TGCCGGC459.1886315E-538.88847719
GCCGTAT556.6856137E-638.1814154
TCGGTGG1051.4551915E-1136.6662845
GGGCGCT708.6763976E-734.9996313
GTAAGCG400.002417140634.9996346
TCGCCGT601.2140888E-534.99962652
GTATCAT957.610652E-933.15754357
CTGCCGG552.9891843E-431.81784218
Produced by FastQC (version 0.11.5)