FastQCFastQC Report
Wed 25 May 2016
SRR1781119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336962
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4185612.421578694333487No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG77532.3008529151655086No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC28280.839263774550246No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC16910.5018370023919612No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT14750.43773481876294656No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT13050.3872840260919629RNA PCR Primer, Index 14 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC10270.30478214160647193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4210.12493990420284778No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG3940.11692713124922098No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT3920.11633359251191529No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3630.10772728082098279No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT3380.10030804660466167No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3380.10030804660466167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAT150.00222413469.99525516
ACCGGTA401.3460522E-1069.9952527
AGGGGGG49750.063.9454170
AATATCG352.97101E-759.9959336
CCGACTT509.786163E-1055.996247
CGTAATC252.3894047E-455.996248
GCCGACT700.054.9962746
CGGTTCA1800.054.44075829
CCGGTAC452.6973794E-854.44075828
CGACTTC653.6379788E-1253.84250348
ACGGTTC653.6379788E-1253.84250328
TGCCGAC653.6379788E-1253.84250345
CGTCTAC608.367351E-1152.4964448
CGAATGC608.367351E-1152.4964441
CGGAAGT200.00694965952.49643766
TCGTTCC200.00694965952.49643762
CCAGCGG200.00694965952.49643739
AATCTCG1600.052.49643736
AACTCGT800.052.49643737
ACGACTA200.00694965952.49643747
Produced by FastQC (version 0.11.5)