FastQCFastQC Report
Wed 25 May 2016
SRR1781118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164313
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7047042.88765952785233No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAACAGTGTAGATCTCGGTG1910.11624156335773797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAG150.002221540869.999721
TCCGACG150.002221540869.999743
TTCCGAC150.002221540869.999742
TTTCCGA150.002221540869.999741
CGCCTTT150.002221540869.999727
ACGGCAT150.002221540869.999747
CGGTGCG150.002221540869.999716
TCGAGTC150.002221540869.999723
TTCGAGT150.002221540869.999722
TCAACCG150.002221540869.999722
ATTTCCG150.002221540869.999740
TGGCGGC252.8302366E-669.99969519
TGTGCGC207.9086116E-569.9996953
CGCTGGC252.3850407E-455.9997616
TCAGAGT252.3850407E-455.9997613
GCGTGTG252.3850407E-455.999768
AACCGCC200.0069416152.4997724
ACACGTC200.0069416152.499776
TACTTCG200.0069416152.4997719
GAATGCC200.0069416152.4997741
Produced by FastQC (version 0.11.5)