FastQCFastQC Report
Wed 25 May 2016
SRR1781118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164313
Sequences flagged as poor quality0
Sequence length76
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT147158.955469135126252No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT43602.6534723363337043No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG18661.1356374723850213No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT9980.6073773834084948No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT9720.5915539245221011No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG8400.5112194409450256No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT6980.42479901164241424No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT6700.40775836361091333No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC6500.3955864721598413No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT6160.3748942566930188No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG5610.3414215552025707No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT5270.32072933973574824No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT4780.29090820568062176No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC4230.25743550419017364No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT4060.24708939645676242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3710.22578858641738633No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT3550.21605107325652873No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCCT3520.21422528953886788No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCG2980.18136118262097337No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCG2660.16188615629925812No Hit
CTTATACACATCTCCGAGCCCACGAGACATCGCGGCATCTCGTATGCCGT2600.15823458886393652No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT2430.14788848113052527No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCACGTATGCCGT2380.14484550826775727No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGG2370.14423691369520367No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGA2360.14362831912265006No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTAAGCCGT2290.13936815711477485No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGG2180.1326736168166852No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT2150.13084783309902442No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT2070.1259790765185956No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCG1980.1205017253656132No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCAAGTATGCCGT1900.11563296878518438No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCC1840.11198140134986276No Hit
CTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC1790.10893842848709474No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCG1660.10102669904389792No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG150.002206897870.116465
TGCCCGA150.002222958569.9884445
ACGTTTG150.002222958569.9884440
ACTGAAT150.002222958569.9884453
TAGCGTA150.002222958569.9884438
ATTACGA150.002222958569.9884444
ATTCGCG150.002222958569.9884428
GCTTGGT150.002222958569.9884413
TAACCGA207.914915E-569.9884411
CGCTCAC150.002222958569.9884410
TCTAATG150.002222958569.9884450
CTTGCGG150.002222958569.9884439
AATCTCG301.0199983E-769.9884436
AATTCGC207.914915E-569.9884427
CCCTTGC150.002222958569.9884437
ATGCGTC150.002222958569.9884445
GGTTGGA150.002222958569.9884456
TGAATAC150.002222958569.9884417
CCTTGCG150.002222958569.9884438
CGCCCCT150.002222958569.9884434
Produced by FastQC (version 0.11.5)