FastQCFastQC Report
Wed 25 May 2016
SRR1781117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407572
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6275515.39727949908237No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGG150.00222396469.9992642
TCTCGGG150.00222396469.9992643
CCGGCGA252.3893244E-455.99941321
TGCCCGG200.00694912352.49944722
CCGGACT200.00694912352.49944725
GGTCTCG200.00694912352.49944741
TGGTCGC751.4551915E-1146.66617649
GCTATAC404.5898472E-543.7495423
CTCGGTG1000.041.9995644
GTCGCCG757.548806E-1041.99955751
TCGCCGT701.9232175E-839.9995852
CATGGAC350.001254880339.9995851
CGTTACT350.001254880339.9995817
GACTGCC350.001254880339.9995845
CTGGAGG350.001254880339.9995831
TCGGTGG1150.039.564845
CGCGGTC459.1920156E-538.8884844
ATTCGAT556.689055E-638.1814232
CTGCCGG556.689055E-638.1814218
TCTCGGT1250.036.39961643
Produced by FastQC (version 0.11.5)