FastQCFastQC Report
Wed 25 May 2016
SRR1781117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407572
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT365808.97510133178923No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG68051.6696436457852843No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC22300.5471425907569706No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT14180.3479139882033113No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT12590.30890247612691746No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC12460.305712855642684No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT11870.29123688575270135Illumina PCR Primer Index 11 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC6990.17150343988301453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTA150.002224304269.9965748
CGTCATA207.924413E-569.9965748
AGGGGGG47850.062.3166970
ACGTATG308.372866E-658.3304740
CACGTAT308.372866E-658.3304739
ACGACGA800.056.8722127
TATGACG1850.056.7539743
GTATGAC1800.056.3861242
GAAGCTA252.389779E-455.99725329
TTAGACC252.389779E-455.99725319
TGCCGTA509.804353E-1055.99725345
CGTATGA2050.054.63146641
TGACGTC1850.052.97037545
CGTCTTA608.367351E-1152.49742548
ATTCCGT200.00695018252.49742544
AATCTCG1600.052.49742536
CGTCTTC61650.051.43300248
GCCGTCT61450.051.3725846
CCGTCTT60900.051.26185647
TATGCCG63350.051.10246743