FastQCFastQC Report
Wed 25 May 2016
SRR1781116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284711
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6314922.180035193582263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGCG150.00222344669.9978944
ACGAGTA150.00222344669.99789412
TGTGCGC308.365469E-658.3315773
GCGTCGG252.3883276E-455.9983148
AGCGCGG200.006947519752.4984232
CGCGTCG200.006947519752.498427
GCGCGTC352.0838548E-549.9984936
TGCGCGT604.9203663E-946.665265
CGGTGTA305.8727834E-446.6652655
GTGCGCG604.9203663E-946.665264
AGTCCGC404.586873E-543.74868442
TTGGCGC350.001254364339.9987955
CGTATCA350.001254364339.99879556
CGATTGT350.001254364339.99879543
CTTGGCG350.001254364339.9987954
GAAAGTC350.001254364339.99879552
GGTGCGC350.001254364339.9987953
TACTCGT350.001254364339.99879560
CGAGAAT350.001254364339.99879514
GTAGTCG350.001254364339.99879550
Produced by FastQC (version 0.11.5)