FastQCFastQC Report
Wed 25 May 2016
SRR1781116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284711
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3941913.845267657378885No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG71862.5239628957082796No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC24290.8531458215523811No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT15750.5531925355887198No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC12400.43552936135238896No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT11940.419372626979639TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC7550.26518118372665617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4150.14576184271067855No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT3910.13733224216837425No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT3450.12117550779562433No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG3440.12082427443969498No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC3300.11590700745668416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTT800.065.6197347
AGGGGGG49350.064.0374170
GCCGACT850.061.7597546
TGCCGAC850.061.7597545
ATATCCG800.061.2450810
GCCGTAT401.0639269E-861.2450846
GCCATAG352.9516195E-760.0479161
AAGCCGT308.367957E-658.3286544
CGACTTC1100.057.2681348
CTTTATA2800.055.0439222
ATCTCGA900.054.44007537
GCCGTCA850.053.52511646
CCATAGA407.4232776E-752.5419242
TGCCGTA608.367351E-1152.4957945
GTCTGCC200.00694889952.49578542
CGTCTAC800.052.49578548
TCAGCAT407.468425E-752.49578553
AACGAGC407.468425E-752.49578513
AACTCGT800.052.49578537
CGGTATG200.00694889952.49578543