FastQCFastQC Report
Wed 25 May 2016
SRR1781115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270660
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6848125.301485258257593No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAACAGTGTAGATCTCGGTG3230.1193379147269637No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG207.9110614E-570.011832
TGCGGGT150.00222320169.998895
CTGTTTT252.3879483E-455.9991125
CGTATCA452.6935595E-854.44358456
GAAGCTC200.006946758452.49917231
TGGACAG200.006946758452.49917227
GGGCGCT200.006946758452.49917213
GCGATTG200.006946758452.49917253
GCCGTAT506.178743E-848.9992254
GTCGCCG604.9167284E-946.6659351
TACTCGC451.6811664E-646.66592820
TCGCCGT551.3077988E-744.54474652
ACTCGCA551.3077988E-744.54474621
GCGCTGC551.3077988E-744.54474615
TGGTCGC659.977157E-943.07623749
GTTCGCT503.4713776E-641.99933211
CATAGAC350.001253027240.0067562
CCTCGTA350.001254166539.99936711
TCGGAGC350.001254166539.99936739
ATCCAAT350.001254166539.99936749
Produced by FastQC (version 0.11.5)