FastQCFastQC Report
Wed 25 May 2016
SRR1781115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270660
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT3796714.02756225522796No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG68802.541934530407153No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC27341.0101234020542378No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT18070.6676272814601345No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC12670.4681149782014335No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT9730.3594916130939186TruSeq Adapter, Index 2 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT8690.3210670213552058No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC6670.24643464124732137No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT6490.23978423113869798No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT5340.19729549988915981No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5130.1895366880957659No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT5030.18584201581319737No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCG4870.1799305401610877No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG3470.1282051282051282No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC2730.10086455331412103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC252.8356571E-669.9977948
CGACTTC252.8356571E-669.9977948
TAACTCG207.918928E-569.9977936
CGAGTTG150.00222334169.9977844
GACTTTA150.00222334169.9977818
ATCGTAA150.00222334169.9977839
CCGAATT150.00222334169.9977847
AACTCGT150.00222334169.9977837
AGGGGGG43950.064.02527670
CATATGC1000.062.99800551
CGTCATA1850.060.53862448
TCGTAAT308.364434E-658.33148640
TTGGCAT252.3881345E-455.99822629
CTCGTTT252.3881345E-455.99822639
GACGTAT509.786163E-1055.99822646
TCTCGTT252.3881345E-455.99822638
CTCGAAA252.3881345E-455.99822639
AGCCGTC1200.055.41491345
TCTTTAT2300.054.801121
TGCCGAC452.6939233E-854.4427245
Produced by FastQC (version 0.11.5)