FastQCFastQC Report
Wed 25 May 2016
SRR1781114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255327
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7614429.82214963556537No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAACAGTGTAGATCTCGGTG3210.1257211340751272No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA150.002222941769.999821
CGGGGAC150.002222941769.999847
TCTCGGG150.002222941769.999843
ACGTGCG150.002222941769.99987
CCTGACG150.002222941769.99983
ACGGTGT150.002222941769.999833
ACGTCGC252.3875365E-455.99984737
GTGCGCG252.3875365E-455.9998474
GATCTAC200.00694057352.5101361
TCGCCGG200.006945952352.4998521
TCCGCGT200.006945952352.4998558
CGAGTGG200.006945952352.4998525
CGATTGG200.006945952352.4998525
TGCGCGG200.006945952352.499855
CCACGTT200.006945952352.4998537
TCGAATC200.006945952352.499858
CGGTGGT900.050.55541246
GTGTCGG352.082907E-549.99986342
CTTATTC506.1761966E-848.99986658
TGACGTG305.8708474E-446.6665345
Produced by FastQC (version 0.11.5)