FastQCFastQC Report
Wed 25 May 2016
SRR1781114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255327
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4651518.21781480219483No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG85493.348255374480568No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC31781.2446783928060878No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT18090.7085032135261841RNA PCR Primer, Index 19 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT15000.5874819349305009No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC14930.5847403525674919No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC9130.35758067106103153RNA PCR Primer, Index 19 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5430.21266846044484133No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAAAATCTCGTATGCCGT5300.20757695034211032No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAATCTCGTATGCCGTC4200.16449494178054025No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG3950.15470357619836525No Hit
CTTATACACATCTCCGAGCCAACGAGACGCGTTAAAATCTCGTATGCCGT3900.1527453030819302RNA PCR Primer, Index 19 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3760.14726213835591223RNA PCR Primer, Index 19 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAAAAATCTCGTATGCCGT3760.14726213835591223No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC3440.1347291904107282No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC3410.13355422654086718No Hit
TATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCT3050.11945466010253518No Hit
CTTATACACATCTCCGAGACCACGAGACGCGTTAAAATCTCGTATGCCGT3040.11906300547924818RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT2900.11357984075323016No Hit
CTTATACACATCTCAGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2890.11318818612994316RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCAGT2780.10887998527378616No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAA150.00222338669.9962857
AGGGGGG58150.063.43600570
AAACTCG750.060.66344536
TAAGCGC352.9678267E-759.9968147
ATCTAGT650.059.22762337
ATCGTAT1050.056.66365439
ATATCGT1050.056.66365437
ACGCGAA252.388131E-455.99702527
CTTAAGC252.388131E-455.99702551
AACGTAT252.388131E-455.99702539
GCGCGCA252.388131E-455.99702531
CTTTATA2600.055.221822
AACTCGT700.054.99707837
TATGACG1150.054.77969443
GACCACG2300.054.77969418
ACGCGTA1600.054.6845927
AATCACG452.6935595E-854.4415536
TGACGTC1100.054.08802845
ATGACGT1100.054.08802844
CGCGATA1850.052.9701628
Produced by FastQC (version 0.11.5)