FastQCFastQC Report
Wed 25 May 2016
SRR1781113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289501
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7103324.536357387366536No Hit
CTTATACACATCTGACGCTGCCGACGACAGGAACAGTGTAGATCTCGGTG3080.10638996065643988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1050.063.33114256
TGCGCGT401.063745E-861.2478835
GCCGTAT1050.059.99792554
GGTCTAA252.3762402E-456.0561521
CGCGTCT509.786163E-1055.9980667
ATCGACA252.3884479E-455.99806651
CGACGAT200.0069477652.49818422
TAACGTT200.0069477652.49818412
ACGATTT200.0069477652.49818431
GCCGACA200.0069477652.49818465
TACGATT200.0069477652.49818430
CTCGGTG1700.051.4688144
TCGGTGG1850.049.18748545
GCGCGTC506.1816536E-848.9983066
TGGTCGC1500.048.99830649
TAGATCT1650.048.7946239
TCTCGGT1850.047.29566243
TCGCCGT1500.046.66505452
CGACGAG305.873077E-446.66505422
CCGTATC1400.044.99844755
Produced by FastQC (version 0.11.5)